Family name: Loi First name:Pasqualino
ORCID: 0000-0003-4631-7663 Date of birth: 15th October 1958
EDUCATION
1991 PhD in Physiology of Reproduction, University of Sassari, Italy
1986 Degree in Veterinary Medicine, University of Sassari, Italy
CURRENT POSITION(S) 2019 January-present: Full Professor, Faculty of Veterinary, University of Teramo, Italy PREVIOUS POSITIONS 2000 - 2018: Associate Professor, Faculty of Veterinary, University of Teramo, Italy 1994 - 2000 October: Senior Scientist, Institute of Zootechnic, Sardinia, Italy
1992 - 1994 Post Doc; Roslin Institute, Edinburgh, Scotland, UK
FELLOWSHIPS AND AWARDS
1994 EMBO Fellow, University of Newcastle upon Tyne, UK
2017 Japanese Society for the Promotion of Science (JSPS) Fellow, Riken Institute, Tsukuba, Japan
SUPERVISION OF GRADUATE STUDENTS AND POSTDOCTORAL FELLOWS
Under graduate students of Veterinary Sciences and Biotechnology of Reproduction (n° 23); PhD students (n°15); Post Docs: (n° 9)
2005 External Evaluator of the PhD program in the Polish Academy of Science, Poland 2008
External Evaluator of the PhD program in Nottingham University, UK
2017 External Evaluator of the PhD program in Bologna University, Italy 2016 External Evaluator of the PhD program in Milan University, Italy;
ORGANISATION OF SCIENTIFIC MEETINGS (last 5 years)
Member of the Scientific and Organizing Committee: International Congress on Animal Reproduction (ICAR), Bologna, June 2022, Italy
Member of the Scientific and Organizing Committee; Workshop "Micromanipulation of oocyte/embryos”, 33rd Meeting of the Association of Embryo Technologies in Europe, Bath, 8-9 September 2017; UK
Member of the Scientific and Organizing Committee; International Conference “Epigenetic Penetrance of Reproductive Technologies”, University of Teramo, 9-10 June 2016, project EU Horizon2020 "EraofArt", Twinning Action.
INSTITUTIONAL RESPONSIBILITIES
2009 - 2011: Scientific Advisory Board, EU ANIMBIOGEN, VII FP EU, Polish Academy of Sciences, Warsaw, Poland.
2007 - present: Review Board member for the European Science Foundation 2008-2011: Deputy of the EU COST “Gemini” Managing Committee –EU VII FP.
2004 - Present: PhD Committee in Veterinary Sciences, University of Teramo
2012 - Present: Member of the Steering Committee for the European Association of European Veterinary Education (EAVE)
2018 - present: Responsible person for students with disabilities of the Faculty of Veterinary Medicine in Teramo
2019 - present: President of the Committee for the Research Assessment, Faculty of Veterinary, University of Teramo.
2020 – present: European Research Council, Executive Agency (ERCEA), Seconded National Experts, Department B, Life Sciences, reserve list.
Editorial Board Member
Animal Science Papers and Reports. ISSN: 0860 4037
Cellular Reprogramming. ISSN: 2152- 4971
Journal of Reproduction and Development”. ISSN: 1348-4400
Frontiers in Cell and Developmental Biology: Electronic ISSN: 2296-634X
Frontiers in Physiology: Electronic ISSN: 1664-042X
Review, ad hoc
Ad hoc reviewer: Nature, Biology of Reproduction, Theriogenology, Gene, Trends in Biotechnology, Cloning and Stem Cells, Reproduction, Fertility and Development, Genomics, Reproduction in Domestic Animals, PLOS ONE, Scientific Reports, etc.
Referee for funding agencies
2007 –Referee for the European Research Council –remote evaluation - (ERC), VII FP EU
2010-2014 Referee for the Marie Curie Fellowship Program, VII FP EU
2011-2019 Referee for the Polish Academy of Sciences
2013 Referee for the Royal Society, UK
2013 Referee for the USA Israel Bi-National Scientific Program
2015-2019 Referee for the Cyprus Academy of Sciences
2017-to present Referee for the European Science Foundation
2018 Referee for the Lithuanian National Science Foundation
2018 Referee for the Medical Research Council, UK
2019 Referee for the Fonds Wetenschappelijk Onderzoek, Belgium
2018-to present Referee for the Dutch Science Foundation
2018-to present Referee for the Czech Academy of Sciences
2010-to present Referee for the Italian Minister of Research and University
2020-to present Referee for the Natural Sciences and Engineering Research Council of Canada.
MEMBERSHIPS OF SCIENTIFIC SOCIETIES
Italian Society of Veterinary Sciences (SISVET); Italian Association of Veterinary Physiologists, Steering Committee (SOFIVET; International Embryo Technology Association (IETS)
Granted Patents
Invited presentations to internationally established conferences (last 5 years):
Short Biographical Sketch
I started to work on embryo micromanipulation, 30 years ago on embryo multiplication by splitting and embryonic cells nuclear transfer during my PhD. Next I joined Keith Campbell and Ian Wilmut at the Roslin Institute, UK, as a Post Doc. The publications jointly delivered (Campbell, KHS Loi et al., Reviews of Reproduction, 1996; Campbell KHS, Loi P., Biol Reprod. 1994) prepared the ground for the production of the first sheep cloned from embryonic (Campbell et al., Nature 1996;380: 64) and adult cells (Wilmut et al., Nature 1997, 385: 810). After my work at Roslin, I continued to work on Somatic Cell Nuclear Transfer (SCNT), producing the first wild animal by cloning (Loi et al. Nature Biotechnology, 2001), even using dead cells (Loi et al., Biol Reprod. 2002), a breakthrough that has prepared the ground for cloning using lyophilized cells (Loi et al., PLOS ONE 2008), and paved the way to the most recent attempt to clone the mammoth (Yamagata et al., Sci Rep. 2019; 9:4050). The latest breakthrough of my research group is to improve nuclear reprogramming by conferring somatic cells the nuclear structure of a spermatid through the heterologous expression of protamine1 (Iuso et al., Cell Reports 2015; Czernik et al., Nature Protocols 2016; Palazzese et al., PLOS ONE, 2018).
Metrics - My H Index is 36 (Google Scholar), with 4450 citations. My H- index and number of citations may appear low; however, I was amongst the first researchers to work on nuclear transfer. For many years the number of groups worldwide on the topics was below 10 so it is not possible to have a high H-index in such circumstances, considering also that I publish on animal science, notoriously with low IF.
Latest Dissemination as influential Scientist in my field https://www.nature.com/news/endangered-species-sex-and-the-single-rhinoceros-1.10731 https://www.scientificamerican.com/article/scientists-successfully-c/ https://www.scientificamerican.com/article/spry-dolly-siblings-could-make-clone-skeptics-sheepishhttps://www.scientificamerican.com/article/cloning-endangered-animals/https://www.scientificamerican.com/article/rhinoceros-assisted-reproduction-rescue-sepcies http://www.nytimes.com/2016/07/27/science/dolly-the-sheep-clones.html?_r=0; https://doi.org/10.1038/nature.2012.10228
Family name: Loi First name:Pasqualino
ORCID: 0000-0003-4631-7663 Date of birth: 15th October 1958
EDUCATION
1991 PhD in Physiology of Reproduction, University of Sassari, Italy
1986 Degree in Veterinary Medicine, University of Sassari, Italy
CURRENT POSITION(S) 2019 January-present: Full Professor, Faculty of Veterinary, University of Teramo, Italy PREVIOUS POSITIONS 2000 - 2018: Associate Professor, Faculty of Veterinary, University of Teramo, Italy 1994 - 2000 October: Senior Scientist, Institute of Zootechnic, Sardinia, Italy
1992 - 1994 Post Doc; Roslin Institute, Edinburgh, Scotland, UK
FELLOWSHIPS AND AWARDS
1994 EMBO Fellow, University of Newcastle upon Tyne, UK
2017 Japanese Society for the Promotion of Science (JSPS) Fellow, Riken Institute, Tsukuba, Japan
SUPERVISION OF GRADUATE STUDENTS AND POSTDOCTORAL FELLOWS
Under graduate students of Veterinary Sciences and Biotechnology of Reproduction (n° 23); PhD students (n°15); Post Docs: (n° 9)
2005 External Evaluator of the PhD program in the Polish Academy of Science, Poland 2008
External Evaluator of the PhD program in Nottingham University, UK
2017 External Evaluator of the PhD program in Bologna University, Italy 2016 External Evaluator of the PhD program in Milan University, Italy;
ORGANISATION OF SCIENTIFIC MEETINGS (last 5 years)
Member of the Scientific and Organizing Committee: International Congress on Animal Reproduction (ICAR), Bologna, June 2022, Italy
Member of the Scientific and Organizing Committee; Workshop "Micromanipulation of oocyte/embryos”, 33rd Meeting of the Association of Embryo Technologies in Europe, Bath, 8-9 September 2017; UK
Member of the Scientific and Organizing Committee; International Conference “Epigenetic Penetrance of Reproductive Technologies”, University of Teramo, 9-10 June 2016, project EU Horizon2020 "EraofArt", Twinning Action.
INSTITUTIONAL RESPONSIBILITIES
2009 - 2011: Scientific Advisory Board, EU ANIMBIOGEN, VII FP EU, Polish Academy of Sciences, Warsaw, Poland.
2007 - present: Review Board member for the European Science Foundation 2008-2011: Deputy of the EU COST “Gemini” Managing Committee –EU VII FP.
2004 - Present: PhD Committee in Veterinary Sciences, University of Teramo
2012 - Present: Member of the Steering Committee for the European Association of European Veterinary Education (EAVE)
2018 - present: Responsible person for students with disabilities of the Faculty of Veterinary Medicine in Teramo
2019 - present: President of the Committee for the Research Assessment, Faculty of Veterinary, University of Teramo.
2020 – present: European Research Council, Executive Agency (ERCEA), Seconded National Experts, Department B, Life Sciences, reserve list.
Editorial Board Member
Animal Science Papers and Reports. ISSN: 0860 4037
Cellular Reprogramming. ISSN: 2152- 4971
Journal of Reproduction and Development”. ISSN: 1348-4400
Frontiers in Cell and Developmental Biology: Electronic ISSN: 2296-634X
Frontiers in Physiology: Electronic ISSN: 1664-042X
Review, ad hoc
Ad hoc reviewer: Nature, Biology of Reproduction, Theriogenology, Gene, Trends in Biotechnology, Cloning and Stem Cells, Reproduction, Fertility and Development, Genomics, Reproduction in Domestic Animals, PLOS ONE, Scientific Reports, etc.
Referee for funding agencies
2007 –Referee for the European Research Council –remote evaluation - (ERC), VII FP EU
2010-2014 Referee for the Marie Curie Fellowship Program, VII FP EU
2011-2019 Referee for the Polish Academy of Sciences
2013 Referee for the Royal Society, UK
2013 Referee for the USA Israel Bi-National Scientific Program
2015-2019 Referee for the Cyprus Academy of Sciences
2017-to present Referee for the European Science Foundation
2018 Referee for the Lithuanian National Science Foundation
2018 Referee for the Medical Research Council, UK
2019 Referee for the Fonds Wetenschappelijk Onderzoek, Belgium
2018-to present Referee for the Dutch Science Foundation
2018-to present Referee for the Czech Academy of Sciences
2010-to present Referee for the Italian Minister of Research and University
2020-to present Referee for the Natural Sciences and Engineering Research Council of Canada.
MEMBERSHIPS OF SCIENTIFIC SOCIETIES
Italian Society of Veterinary Sciences (SISVET); Italian Association of Veterinary Physiologists, Steering Committee (SOFIVET; International Embryo Technology Association (IETS)
Granted Patents
Invited presentations to internationally established conferences (last 5 years):
Short Biographical Sketch
I started to work on embryo micromanipulation, 30 years ago on embryo multiplication by splitting and embryonic cells nuclear transfer during my PhD. Next I joined Keith Campbell and Ian Wilmut at the Roslin Institute, UK, as a Post Doc. The publications jointly delivered (Campbell, KHS Loi et al., Reviews of Reproduction, 1996; Campbell KHS, Loi P., Biol Reprod. 1994) prepared the ground for the production of the first sheep cloned from embryonic (Campbell et al., Nature 1996;380: 64) and adult cells (Wilmut et al., Nature 1997, 385: 810). After my work at Roslin, I continued to work on Somatic Cell Nuclear Transfer (SCNT), producing the first wild animal by cloning (Loi et al. Nature Biotechnology, 2001), even using dead cells (Loi et al., Biol Reprod. 2002), a breakthrough that has prepared the ground for cloning using lyophilized cells (Loi et al., PLOS ONE 2008), and paved the way to the most recent attempt to clone the mammoth (Yamagata et al., Sci Rep. 2019; 9:4050). The latest breakthrough of my research group is to improve nuclear reprogramming by conferring somatic cells the nuclear structure of a spermatid through the heterologous expression of protamine1 (Iuso et al., Cell Reports 2015; Czernik et al., Nature Protocols 2016; Palazzese et al., PLOS ONE, 2018).
Metrics - My H Index is 35 (Google Scholar), with 4250 citations. My H- index and number of citations may appear low; however, I was amongst the first researchers to work on nuclear transfer. For many years the number of groups worldwide on the topics was below 10 so it is not possible to have a high H-index in such circumstances, considering also that I publish on animal science, notoriously with low IF.
Latest Dissemination as influential Scientist in my field https://www.nature.com/news/endangered-species-sex-and-the-single-rhinoceros-1.10731 https://www.scientificamerican.com/article/scientists-successfully-c/ https://www.scientificamerican.com/article/spry-dolly-siblings-could-make-clone-skeptics-sheepishhttps://www.scientificamerican.com/article/cloning-endangered-animals/https://www.scientificamerican.com/article/rhinoceros-assisted-reproduction-rescue-sepcies http://www.nytimes.com/2016/07/27/science/dolly-the-sheep-clones.html?_r=0; https://doi.org/10.1038/nature.2012.10228
1. Saragusty J, Loi P (2019) Exploring dry storage as an alternative biobanking strategy inspired by Nature. Theriogenology 126:17-27. doi: 10.1016/j.theriogenology.2018.11.027
*2. Anzalone DA, Palazzese L, Iuso D, Martino G, Loi P (2018) Freeze-dried spermatozoa: An alternative biobanking option for endangered species. Anim Reprod Sci 190:85-93. doi: 10.1016/j.anireprosci.2018.01.010
3. Hildebrandt TB, Hermes R, Colleoni S, Diecke S, Holtze S, Renfree MB, Stejskal J, Hayashi K, Drukker M, Loi P, Göritz F, Lazzari G, Galli C (2018) Embryos and embryonic stem cells from the white rhinoceros. Nat Commun 9 (1):2589. doi: 10.1038/s41467-018-04959-2
4. Loi P, Galli C, Lazzari G, Matsukawa K, Fulka Jr J, Goeritz F, Hildebrandt TB (2018) Development to term of sheep embryos reconstructed after inner cell mass/trophoblast exchange. J Reprod Dev 64 (2):187-191. doi: 10.1262/jrd.2017-109
*5. Palazzese L, Czernik M, Iuso D, Toschi P, Loi P (2018) Nuclear quiescence and histone hyper-acetylation jointly improve protamine-mediated nuclear remodeling in sheep fibroblasts. PLOS ONE 13 (3):e0193954. doi: 10.1371/journal.pone.0193954
*6. Palazzese L, Gosálvez J, Anzalone DA, Loi P, Saragusty J (2018) DNA fragmentation in epididymal freeze-dried ram spermatozoa impairs embryo development. J Reprod Dev 64 (5):393-400. doi: 10.1262/jrd.2018-033
7. Capra E, Toschi P, Del Corvo M, Lazzari B, Scapolo PA, Loi P, Williams JL, Stella A, Ajmone-Marsan P (2017) Genome-wide epigenetic characterization of tissues from three germ layers isolated from sheep fetuses. Front Genet 8:Article 115. doi: 10.3389/fgene.2017.00115
8. Loi P, Iuso D, Toschi P, Palazzese L, Czernik M (2017) Alternative strategies for nuclear reprogramming in somatic cell nuclear transfer (SCNT). Anim Reprod 14 (2):377-382. doi: 10.21451/1984-3143-AR915
9. Anzalone DA, Iuso D, Czernik M, Ptak G, Loi P (2016) Plasma membrane and acrosome loss before ICSI is required for sheep embryonic development. J Assist Reprod Genet 33 (6):757-763. doi: 10.1007/s10815-016-0709-1
*10. Czernik M, Iuso D, Toschi P, Khochbin S, Loi P (2016) Remodeling somatic nuclei via exogenous expression of protamine 1 to create spermatid-like structures for somatic nuclear transfer. Nat Protoc 11 (11):2170-2188. doi: 10.1038/nprot.2016.130
11. Loi P, Iuso D, Czernik M, Ogura A (2016) A new, dynamic era for somatic cell nuclear transfer? Trends Biotech 34 (10):791-797. doi: 10.1016/j.tibtech.2016.03.008
12. Loi P, Toschi P, Zacchini F, Ptak G, Scapolo PA, Capra E, Stella A, Marsan PA, Williams JL (2016) Synergies between assisted reproduction technologies and functional genomics. Genetics Selection Evolution 48 (1):53. doi: 10.1186/s12711-016-0231-z
13. Saragusty J, Diecke S, Drukker M, Durrant B, Friedrich Ben-Nun I, Galli C, Göritz F, Hayashi K, Hermes R, Holtze S, Johnson S, Lazzari G, Loi P, Loring JF, Okita K, Renfree MB, Seet S, Voracek T, Stejskal J, Ryder OA, Hildebrandt TB (2016) Rewinding the process of mammalian extinction. Zoo Biol 35 (4):280-292. doi: 10.1002/zoo.21284
14. Toschi P, Czernik M, Zacchini F, Fidanza A, Loi P, Ptak GE (2016) Evidence of placental autophagy during early pregnancy after transfer of in vitro produced (IVP) sheep embryos. PLOS ONE 11 (6):e0157594. doi: 10.1371/journal.pone.0157594
15. Cree L, Loi P (2015) Mitochondrial replacement: From basic research to assisted reproductive technology portfolio tool-technicalities and possible risks. Molecular Human Reproduction 21 (1):3-10. doi: 10.1093/molehr/gau082
*16. Iuso D, Czernik M, Toschi P, Fidanza A, Zacchini F, Feil R, Curtet S, Buchou T, Shiota H, Khochbin S, Ptak Grazyna E, Loi P (2015) Exogenous expression of human protamine 1 (hPrm1) remodels fibroblast nuclei into spermatid-like structures. Cell Reports 13 (9):1765-1771. doi: 10.1016/j.celrep.2015.10.066
17. Cree L, Loi P (2014) Mitochondrial replacement: From basic research to assisted reproductive technology portfolio tool—technicalities and possible risks. Molecular Human Reproduction 21 (1):3-10. doi: 10.1093/molehr/gau082
18. Fidanza A, Toschi P, Zacchini F, Czernik M, Palmieri C, Scapolo P, Modlinski JA, Loi P, Ptak GE (2014) Impaired placental vasculogenesis compromises the growth of sheep embryos developed in vitro. Biol Reprod 91 (1):Article 21, 21-27. doi: 10.1095/biolreprod.113.113902
19. Loi P, Saragusty J, Ptak G (2014) Cloning the mammoth: A complicated task or just a dream? In: Holt WV, Brown JL, Comizzoli P (eds) Reproductive Sciences in Animal Conservation, vol 753. Advances in Experimental Medicine and Biology. Springer New York, pp 489-502. doi: 10.1007/978-1-4939-0820-2_19
20. Ptak GE, Toschi P, Fidanza A, Czernik M, Zacchini F, Modlinski JA, Loi P (2014) Autophagy and apoptosis: Parent-of-origin genome-dependent mechanisms of cellular self-destruction. Open Biology 4 (6):140027. doi: 10.1098/rsob.140027
21. Sampino S, Juszczak GR, Zacchini F, Swiergiel AH, Modlinski JA, Loi P, Ptak GE (2014) Grand-paternal age and the development of autism-like symptoms in mice progeny. Transl Psychiatry 4:e386. doi: 10.1038/tp.2014.27
22. Zacchini F, Loi P, Sampino S, Ptak GE, Swiergiel AH, Modlinski AJ (2014) Effects of blastomere biopsy on post-natal growth and behavior in mice. Hum Reprod 29 (9):1875-1883. doi: 10.1093/humrep/deu145
Loi P, Czernik M, Zacchini F, Iuso D, Scapolo PA, Ptak G (in stampa). Sheep: The First Large Animal Model in Nuclear Transfer Research. CELLULAR REPROGRAMMING, ISSN: 2152-4971
S Sampino, G R Juszczak, F Zacchini, A H Swiergiel, J A Modlinski, P Loi, G E Ptak (2014). Grand-paternal age and the development of autism-like symptoms in mice progeny. TRANSLATIONAL PSYCHIATRY, vol. 4, ISSN: 2158-3188, doi: 10.1038/tp.2014.27
Iuso D, Czernik M, Zacchini F, Ptak G, Loi P (2013). A simplified approach for oocyte enucleation in mammalian cloning.. CELLULAR REPROGRAMMING, vol. 15, ISSN: 2152-4971, doi: 10.1089/cell.2013.0051
Czernik M, Fidanza A, Sardi M, Galli C, Brunetti D, Malatesta D, Della Salda L, Matsukawa K, Ptak G, Loi P (2013). Differentiation potential and GFP labeling of sheep bone marrow derived mesenchymal stem cells.. JOURNAL OF CELLULAR BIOCHEMISTRY, vol. 114, p. 134-143, ISSN: 0730-2312, doi: 10.1002/jcb.24310.
Ptak GE, Modlinski JA, Loi P (2013). Embryonic diapause in humans: time to consider?. REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, vol. 11, ISSN: 1477-7827, doi: 10.1186/1477-7827-11-92
Iuso D, Czernik M, Di Egidio F, Sampino S, Zacchini F, Bochenek M, Smorag Z, Modlinski JA, Ptak G, Loi P (2013). Genomic stability of lyophilized sheep somatic cells before and after nuclear transfer. PLOS ONE, vol. Epub 2013 Jan 8., p. 1-10, ISSN: 1932-6203, doi: 10.1371/journal.pone.0051317
Iuso D, Czernik M, Di Egidio F, Sampino S, Zacchini F, Bochenek M, Smorag Z, Modlinski JA, Ptak G, Loi P (2013). Genomic stability of lyophilized sheep somatic cells before and after nuclear transfer.. PLOS ONE, vol. 8, ISSN: 1932-6203, doi: 10.1371/journal.pone.0051317
Ptak GE, D'Agostino A, Toschi P, Fidanza A, Zacchini F, Czernik M, Monaco F, Loi P (2013). Post-implantation mortality of in vitro produced embryos is associated with DNA methyltransferase 1 dysfunction in sheep placenta. HUMAN REPRODUCTION, vol. 28, p. 298-305, ISSN: 0268-1161, doi: 10.1093/humrep/des397
Ptak GE, D'Agostino A, Toschi P, Fidanza A, Zacchini F, Czernik M, Monaco F, Loi P (2013). Post-implantation mortality of in vitro produced embryos is associated with DNA methyltransferase 1 dysfunction in sheep placenta.. HUMAN REPRODUCTION, vol. 28, ISSN: 0268-1161, doi: 10.1093/humrep/des397
Ptak G, D'Agostino Antonella, Toschi Paola, Fidanza Antonella, Zacchini Federica, Marta Czernik, Monaco Federica, Loi Pasqualino (2013). Postimplantational mortality of in vitro produced embryos is associated with DNA methyltransferase 1 (DNMT1) dysfunction in placenta.. HUMAN REPRODUCTION, ISSN: 0268-1161, doi: 10.1093/humrep/des397
allegati
Loi P, Czernik M, Zacchini F, Iuso D, Scapolo PA, Ptak G (2013). Sheep: the first large animal model in nuclear transfer research.. CELLULAR REPROGRAMMING, vol. 15, ISSN: 2152-4971, doi: 10.1089/cell.2013.0032
Loi P., Czernik M., Zacchini F., Iuso D., Scapolo P.A., Ptak G. (2013). Sheep: the first large model in nuclear transfer research. HUMAN REPRODUCTION, vol. 15, p. 367-373, ISSN: 0268-1161, doi: 10.1089/cell.2013.0032
Fulka J Jr, Langerova A, Loi P, Ptak G, Albertini D, Fulka H (2013). The ups and downs of somatic cell nucleus transfer (SCNT) in humans.. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, vol. 30, ISSN: 1058-0468, doi: 10.1007/s10815-013-0053-7
Ptak G, Zacchini F, Czernik M, Fidanza A, Palmieri C, Della Salda L, Scapolo PA, Loi P (2012). A short exposure to polychlorinated biphenyls deregulates cellular autophagy in mammalian blastocyst in vitro.. HUMAN REPRODUCTION, vol. 27, p. 1034-1042, ISSN: 0268-1161, doi: 10.1093/humrep/der454
allegati
Ptak G, Tacconi E, Czernik M, Toschi P, Modlinski JA, Loi P (2012). Embryonic diapause is conserved across mammals.. PLOS ONE, vol. 7, p. 1-7, ISSN: 1932-6203, doi: 10.1186/1477-7827-11-92
Loi P, Teruhiko Wakayama, Josef Fulka Jr and Grazyna Ptak. (2011). Biological time machines: a realistic approach for cloning an extinct mammal.. ENDANGERED SPECIES RESEARCH, vol. 14, p. 227-233, ISSN: 1613-4796
Zacchini F, Czernik M, Iuso D, Toschi P, di Egidio F, Scapolo P.A., Loi P, Ptak G (2011). Efficient Production and Cellular Characterization of Sheep Androgenetic Embryos. CELLULAR REPROGRAMMING, vol. Dec;13(6), p. 495-502, ISSN: 2152-4971, doi: 10.1089/cell.2011.0021
Loi P, Fulka J Jr, Hildebrand T, Ptak G (2011). Genome of non-living cells: trash or recycle?. REPRODUCTION, vol. 76, p. 217-228, ISSN: 1470-1626
Loi P, Modlinski JA, Ptak G (2011). Interspecies somatic cell nuclear transfer: a salvage tool seeking first aid.. THERIOGENOLOGY, vol. 76, p. 217-228, ISSN: 0093-691X
Fulka J Jr, Langerova A, Loi P, Martinkova S, Fulka H. (2011). Transplantation of nucleoli into human zygotes: not as simple as expected? . JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, vol. 28(5), p. 385, ISSN: 1058-0468
FULKA JR J, LOI P, PTAK G, FULKA H, JOHN JS (2009). Hope for the Mammoth?. CLONING AND STEM CELLS, ISSN: 1536-2302
LOI P, BEAUJEAN N, KHOCHBIN S, FULKA J JR, PTAK G (2008). Asymmetric nuclear reprogramming in somatic cell nuclear transfer?. BIOESSAYS, vol. 30, p. 66-74, ISSN: 0265-9247
LOI P, GALLI C, PTAK G (2008). Cloning of endangered mammalian species: any progress?. TRENDS IN BIOTECHNOLOGY, vol. 25, p. 195-200, ISSN: 0167-7799
FULKA J JR, FULKA H, ST JOHN J, GALLI C, LAZZARI G, LAGUTINA I, FULKA J, LOI P (2008). Cybrid human embryos--warranting opportunities to augment embryonic stem cell research. TRENDS IN BIOTECHNOLOGY, vol. 26, p. 469-474, ISSN: 0167-7799
LOI P, MATSUKAWA K, PTAK G, CLINTON M, FULKA J JR, NATHAN Y, ARAV A (2008). Freeze-dried somatic cells direct embryonic development after nuclear transfer. PLOS ONE, vol. e2978., ISSN: 1932-6203
TURCO MY, MATSUKAWA K, CZERNIK M, GASPERI V, BATTISTA N, DELLA SALDA L, SCAPOLO P.A., LOI P, MACCARRONE M, PTAK G. HUMAN REPROD. OCT, - (2008). High levels of anandamide, an endogenous cannabinoid, block the growth of sheep preimplantation embryos by inducing apoptosis and reversible arrest of cell proliferation. HUMAN REPRODUCTION, vol. 23, p. 2331-2338, ISSN: 0268-1161, doi: 10.1093/humrep/den258
Loi P, Matsukawa K, Takahashi S, Akagi S, Ptak G (2008). Nuclear reprogramming: what has been done and potential avenues for improvements. CENTRAL EUROPEAN JOURNAL OF BIOLOGY, vol. 3, p. 211-223, ISSN: 1895-104X
LOI P, MATZUKAWA K, PTAK G, NATAN Y, FULKA J JR, ARAV A (2008). Nuclear transfer of freeze-dried somatic cells into enucleated sheep oocytes. REPRODUCTION IN DOMESTIC ANIMALS, vol. 43, p. 417-422, ISSN: 0936-6768
PALMIERI C, LOI P, PTAK G, DELLA SALDA L (2008). Review paper: a review of the pathology of abnormal placentae of somatic cell nuclear transfer clone pregnancies in cattle, sheep, and mice. VETERINARY PATHOLOGY, vol. 45, p. 865-880, ISSN: 0300-9858
LOI P, GALLI C, PTAK G (2007). Cloning of endangered mammalian species: any progress?. TRENDS IN BIOTECHNOLOGY, vol. 25, p. 195-200, ISSN: 0167-7799
Matsukawa K, Turco MY, Scapolo PA, Reynolds L, Ptak G, Loi P (2007). Development of sheep androgenetic embryos is boosted following transfer of male pronuclei into androgenetic hemizygotes. . CLONING AND STEM CELLS, vol. 9, p. 374-381, ISSN: 1536-2302, doi: 10.1089/clo.2006.0016
PALMIERI C, LOI P, REYNOLDS LP, PTAK G, DELLA SALDA (2007). Placental abnormalities in ovine somatic cell clones at term: a light and electron microscopic investigation. PLACENTA, vol. 28, p. 577-584, ISSN: 0143-4004, doi: 10.1016/j.placenta.2006.08.003
PTAK G, MATSUKAWA K, PALMIERI C, DELLA SALDA L, SCAPOLO PA, LOI P (2006). Developmental and functional evidence of nuclear immaturity in prepubertal oocytes. HUMAN REPRODUCTION, vol. 21 (9), p. 2228-2237, ISSN: 0268-1161, doi: 10.1093/humrep/del184
PTAK G, LOPES F., MATSUKAWA K., TISCHNER M., LOI P. (2006). Leukaemia inhibitory factor enhances sheep fertilization in vitro via an influence on the oocyte. THERIOGENOLOGY, vol. 65, p. 1891-1899, ISSN: 0093-691X
GRAZYNA PTAK, LOI P, FEDERICA LOPES, KAZUTSUGU MATSUKAWA, MARIAN TISCHNER, PASQUALINO LOI (2006). Leukemia Inhibitory Factor enhances sheep ferilization in vitro via an influence on the oocyte. THERIOGENOLOGY, p. 1-10, ISSN: 0093-691X
LOI P., CLINTON M., VACKOVA I., FULKA JR., J., FEIL R., PALMIERI, DELLA SALDA L., PTAK G (2006). Placental abnormalities associated with post-natal mortality in sheep somatic cell clones. THERIOGENOLOGY, vol. 65, p. 1110-1121, ISSN: 0093-691X, doi: 10.1016/j.theriogenology.2005.07.016
PASQUALINO LOI, MICHAEL CLINTON, IRENA VACKOVA, JOSEF FULKA JR, ROBERT FEIL, CHIARA PALMIERI, LEONARDO DELLA SALDA, PTAK G (2006). Placental abnormalities associated with post-natal mortality in sheep somatic cell clones. THERIOGENOLOGY, vol. 65, p. 1110-1121, ISSN: 0093-691X, doi: 10.1016/j.theriogenology.2005.07.016
IRENA VACKOVA, RADOMIR KREN, PASQUALINO LOI, VLADIMR KRYLOV AND JOSEF FULKA, LOI P (2006). The absence of DNA replication checkpoint in porcine zygotes. ZYGOTE, vol. 14, p. 33-37, ISSN: 0967-1994
FULKA J JR, MRAZEK M, FULKA H, LOI P (2005). Mammalian oocyte therapies. CLONING AND STEM CELLS, vol. 7, p. 183-188, ISSN: 1536-2302
LOI P (2004). Are there realistic opportunities to apply cloning and the other reproductive biotechnologies for safeguarding endangered species?. ANIMAL SCIENCE PAPERS AND REPORTS, vol. 22, p. 81-89, ISSN: 0860-4037
FULKA J JR, LOI P, FULKA H, PTAK G, NAGAI T. (2004). Nucleus transfer in mammals: noninvasive approaches for the preparation of cytoplasts. TRENDS IN BIOTECHNOLOGY, vol. 22, p. 279-283, ISSN: 0167-7799
Mattioli M, Gioia L, Turriani M, Capacchietti G, Loi P, Ptak G (2004). Oocyte maturation is required for correct sperm chromatin rearrangement. VETERINARY RESEARCH COMMUNICATIONS, vol. 28 (1), p. 205-208, ISSN: 0165-7380
LOI P., DELLA SALDA L, PTAK G (2004). Peri- and post-natal mortality of somatic cell clones in sheep. ANIMAL SCIENCE PAPERS AND REPORTS, vol. 22, suppl. 1, p. 59-70, ISSN: 0860-4037
BEAUJEAN N, TAYLOR JE, MCGARRY M, GARDNER JO, WILMUT I, LOI P, PTAK G, GALLI C, LAZZARI G, BIRD A, YOUNG LE, MEEHAN RR (2004). The effect of interspecific oocytes on demethylation of sperm DNA. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 20, p. 7636-7640, ISSN: 1091-6490
LOI P, FULKA J JR, PTAK G (2003). Amphibian and mammal somatic-cell cloning: different species, common results? (Cover page). TRENDS IN BIOTECHNOLOGY, vol. 21, p. 471-473, ISSN: 0167-7799
MATTIOLI M, BARBONI B, GIOIA L, LOI P (2003). Cold-induced calcium elevation triggers DNA fragmentation in immature pig oocytes. MOLECULAR REPRODUCTION AND DEVELOPMENT, vol. 65(3), p. 289-297, ISSN: 1040-452X, doi: 10.1002/mrd.10275
YOUNG LE, SCHNIEKE AE, MCCREATH KJ, WIECKOWSKI S, KONFORTOVA G, FERNANDES K, PTAK G, KIND AJ, WILMUT I, LOI P, FEIL R. (2003). Conservation of IGF2-H19 and IGF2R imprinting in sheep: effects of somatic cell nuclear transfer. MECHANISMS OF DEVELOPMENT, vol. 120, p. 1433-1442, ISSN: 0925-4773
PTAK GRAZYNA, TISCHENR MARIAN, BERNABÒ NICOLA, LOI P (2003). Donor-Dependent Developmental Competence of Oocytes from Lambs Subjected to Repeated Hormonal Stimulation. BIOLOGY OF REPRODUCTION, vol. 69 (1), p. 278-285, ISSN: 0006-3363
FULKA J, MOOR RM, LOI P, FULKA J (2003). Enucleolation of porcine oocytes. THERIOGENOLOGY, vol. 59, p. 1879-1885, ISSN: 0093-691X
FULKA J JR, LOI P, FULKA H, KREN R, DEAN W, MRAZEK M, REIK W (2003). Nucleus replacement in Mammalian oocytes. CLONING AND STEM CELLS, vol. 4, p. 181-187, ISSN: 1536-2302
LOI P, BARBONI B, PTAK G (2002). Cloning advances and challenges for conservation. TRENDS IN BIOTECHNOLOGY, vol. 20, p. 233, ISSN: 0167-7799, doi: 10.1016/S0167-7799(02)01955-8
PTAK G., CLINTON M., TISCHNER M., BARBONI B., MATTIOLI M, LOI P. (2002). Improving delivery and offspring viability of in vitro-produced and cloned sheep embryos. BIOLOGY OF REPRODUCTION, vol. 67(6), p. 1719-1725, ISSN: 0006-3363, doi: 10.1095/biolreprod66.3.796
PLOI P, CLINTON M., BARBONI B., FULKA J. JR., CAPPAI P., FEIL R., MOOR R.H.M., PTAK G. (2002). Nuclei of nonviable ovine somatic cells develop into lambs after nuclear transplantation.. BIOLOGY OF REPRODUCTION, vol. 67, p. 126-132, ISSN: 0006-3363, doi: 10.1095/biolreprod67.1.126
PTAK G., CLINTON M., BARBONI B., MUZZEDDU M., CAPPAI P., TISCHNER M., LOI P (2002). Preservation of the wild European mouflon: the first example of genetic management using a complete program of reproductive biotechnologies. BIOLOGY OF REPRODUCTION, vol. 66, p. 796-801, ISSN: 0006-3363, doi: 10.1095/biolreprod66.3.796
LOI P, PTAK G, BARBONI BARBARA, FULKA JOSEF JR, CAPPAI PIETRO, MICHAEL CLINTON (2001). Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells. NATURE BIOTECHNOLOGY, vol. 19, p. 962-964, ISSN: 1087-0156
FULKA J. JR, LOI P, LEDDA S, MOOR RHM, FULKA J (2001). Nucleus transfer in mammals: how the oocyte cytoplasm modifies the transferred nucleus. THERIOGENOLOGY, vol. 55, p. 1373-1380, ISSN: 0093-691X
J. FULKA JR, J. TESARIK, LOI P, R.M. MOOR (2000). Manipulating the Human embryo: Cell Cycle Checkpoint Controls. CLONING AND STEM CELLS, vol. 2, p. 1-7, ISSN: 1536-2302
FULKA J JR, TESARÍK J, LOI P, MOOR RM (2000). Manipulating the human embryo: cell cycle checkpoint controls. CLONING AND STEM CELLS, vol. 2, p. 1-7, ISSN: 1536-2302
PLOI P, FULKA J.JR., NAITANA S., CAPPAI P. (1999). Cloning by nuclear transfer: experiences in sheep. LIVESTOCK PRODUCTION SCIENCE, vol. 60, p. 281-294, ISSN: 0301-6226
PTAK G, LOI P., DATTENA M., TISCHNER M., CAPPAI P. (1999). Offspring from one-month-old lambs: studies on the developmental capability of prepubertal oocytes. BIOLOGY OF REPRODUCTION, vol. 61, p. 1568-1574, ISSN: 0006-3363
PTAK G., DATTENA M., LOI P, TISCHNER M., CAPPAI P (1999). Ovum pick-up in sheep: efficiency of in vitro embryo production, vitrification and offspring. THERIOGENOLOGY, vol. 52, p. 1105-1114, ISSN: 0093-691X
LOI P, PTAK G., DATTENA M., LEDDA S., NAITANA S., CAPPAI P (1998). 26.Embryo transfer and related technologies in sheep reproduction. REPRODUCTION NUTRITION DEVELOPMENT, vol. 38, p. 615-628, ISSN: 0926-5287
FULKA J. JR. FIRST N. L., LOI P, MOOR R. M (1998). Cloning by somatic cell nuclear transfer. BIOESSAYS, vol. 20, p. 847-851, ISSN: 0265-9247
LOI P, LEDDA S., FULKA J.JR., CAPPAI P., MOOR R.M. (1998). Development of parthenogenetic and cloned ovine embryos: effect of activation protocols. BIOLOGY OF REPRODUCTION, vol. 58, p. 1177-1187, ISSN: 0006-3363
FEIL R, KOSHLA S, CAPPAI P, LOI P (1998). Genomic imprinting in ruminants: allele-specific gene expression in parthenogenetic sheep. MAMMALIAN GENOME, vol. 9, p. 831-834, ISSN: 0938-8990
NAITANA S., LEDDA S., LEONI G., BOGLIOLO L., LOI P, CAPPAI P (1998). Membrane integrity and fertilizing potential of cryopreserved spermatozoa in European mouflon. ANIMAL REPRODUCTION SCIENCE, vol. 52, p. 105-112, ISSN: 0378-4320
LEDDA S, BOGLIOLO L, LEONI G, LOI P, CAPPAI P, NAITANA S (1998). Two culture systems showing a biphasic effect on ovine embryo development from the 1-2 cell stage to hatched blastocysts. REPRODUCTION NUTRITION DEVELOPMENT, vol. 35, p. 629-637, ISSN: 0926-5287
CAMPBELL K.H.S., LOI P, OTAEGUI P., WILMUT I (1997). Cell cycle co-ordination in embryo cloning by nuclear transfer. JOURNAL OF REPRODUCTION AND FERTILITY, vol. 1, p. 40-46, ISSN: 0022-4251
LOI P, BOYAZOGLU S., GALLUS M., BRANCA A., LEDDA S., NAITANA S., WILMUT I., CAPPAI P (1997). Embryo Cloning in Sheep: work in progress. THERIOGENOLOGY, vol. 48, p. 1-10, ISSN: 0093-691X
NAITANA S, LEDDA S, LOI P, LEONI G, BOGLIOLO L, DATTENA M, CAPPAI P (1997). Polyvinyl alcohol as a defined substitute for serum in vitrification and warming solutions to cryopreserve ovine embryos at different stages of development. ANIMAL REPRODUCTION SCIENCE, vol. 48, p. 247-256, ISSN: 0378-4320
CAMPBELL KH, LOI P, OTAEGUI PJ, WILMUT I (1996). Cell cycle co-ordination in embryo cloning by nuclear transfer. REVIEWS OF REPRODUCTION, vol. 1, p. 40-46, ISSN: 1359-6004
NAITANA S, LOI P, LEDDA S, CAPPAI P, DATTENA M, BOGLIOLO L, LEONI G (1996). Effect of biopsy and vitrification on in vitro survival of ovine embryos at different stages of development. THERIOGENOLOGY, vol. 46, p. 813-824, ISSN: 0093-691X
LEDDA S., LOI P, BOGLIOLO L., MOOR R.M. FULKA J. JR. (1996). The effect of 6- Dimethylaminopurine (6-DMAP) on DNA synthesis in activated mammalian oocytes. ZYGOTE, vol. 4, p. 7-9, ISSN: 0967-1994
CAMPBELL K.H.S., LOI P, CAPPAI P., WILMUT I (1994). Improved development to blastocyst of ovine nuclear transfer embryos reconstructed during the presumptive S-Phase of enucleated-activated oocytes. BIOLOGY OF REPRODUCTION, vol. 50, p. 1385-1393, ISSN: 0006-3363
Funding ID
COORDINATOR, H2020, MSCA RISE 2016-2021 GA 734434. Setting an interdisciplinary/sectorial/international research network to explore dry storage as an alternative strategy for cells/germplasm biobanking. 1530000 €
COORDINATOR,H2020 MSCA IF 2017-2019. GA 749537, Drystore. Exploring dry storage as an alternative biobanking strategy inspired by Nature. 188000 €
PI H2020 Twinning Action 2016-2019. GA 698165. Epigenetic Risk Assessment of Assisted Reproductive Technologies. 295000 €
COORDINATOR, MIUR PRIN 2009: I 2009JE3CHM Induction and evaluation of multipotency in embryonic… of veterinary interest, 370,000 €
COORDINATOR,MIUR PRIN 2006 Project: 2006078015_002 Cell therapy: development of large animal models for bio-medical and pre-clinical studies, 147000 €
COORDINATOR,PMIUR PRIN 2002: cod. 2002074357 Isolation and characterization of adult and embryonic stem cells… differentiation plasticity 115,000 €
COORDINATOR,MIUR FIRB Project Negotiating Projects 2001: RBNE01HPMX Identification of mechanisms that regulate the expression of genes of … of domestic animals . 1250000 €
PI Project FP7-KBBE-2009-3: Next generation methods to preserve farm animal biodiversity by optimizing present and future breeding options. 135,000 €
PI European Science Foundation grant "Stellar" 2004-007 "Novel approaches to enhance…. research" acronym; EuroSTELLS, VI FP EU contract no. ERAS-CT-2003-980409; 154000 €
COORDINATOR, MIUR Interlink: II04C0H723 Study of the expression of single genes (DNMT1), and of the…. post implantation period. 45000 €
PI United States Department of Agriculture (USDA) "Proposal # 2007-01215
"Deregulation of Placenta vascular development in ART-derived sheep fetuses, $ 350,000
PI MIUR FIRB Establishing a Genome Resort for animal breeding and selection. GenHome cod. 0070120. 678,000 €
COORDINATOR,Tercas Foundation "Development of protocols for the freeze-drying of cell lines and embryos ", 100,000 €
