Prof. CZERNIK Marta

Curriculum accademico

Curriculum english version

Education: 2° PhD (2015): University of Teramo, Department of Comparative Biomedical Sciences, Teramo, Italy; 1° PhD (2012): Jagiellonian University, Medical College, Krakow, Poland and Department of Neuroscience, Centre for Neurovirology, Temple University, Philadelphia, USA; MS (2004): Jagiellonian University, Cell Biology Department, Krakow, Poland.


Work Experience: 2014 - RIKEN, Bioresource Engineering Division, Tsukuba, Japan (JSPS Fellowship); 2007- present: Faculty of Veterinary, Teramo University, Teramo, Italy; 2004-2007: Department of Neuroscience, Centre for Neurovirology, Temple University, Philadelphia, USA.

Number of publications: 37,

Book chapters: 2;

Patents: 2014 Co-inventor of patent “Direct protaminisation of somatic cells improves SCNT efficiency”;

Major Awards: 2016 - Prize for best poster “Exogenous Expression of Human Protamine 1 (hPrm1) Remodels Fibroblast Nuclei into Spermatid-like Structures” awarded by Sir Gurdon (Nobel Prize in Physiology or Medicine 2012) during the International Symposium on Future of Nuclear transfer and Nuclear Reprogramming 10 March in Yamanashi (Japan); 2013 - Japan Society forthe Promotion of Science, Postdoctoral Fellowship for Foreign Researchers (RIKEN, Tsukuba, Japan); 2013 - Research Grant from JSPS for Postdoctoral Fellowship for Foreign Researchers (at Prof. A. Ogura laboratory);


Czernik’s H index is 15, with over 603 citations.

Czernik’s full publications list is available on:



  1. Somatic Cell Nuclear Transfer: Failures, Successes and the Challenges AheadCzernik, Anzalone DA, Palazesse L, Oikawa M, Loi P. International Journal of Developmental Biology J. Dev. Biol. 63: 123 - 130 (2019)doi: 10.1387/ijdb.180324m, IF= 2,4
  2. Nuclear quiescence and histone hyper-acetylation jointly improve protamine-mediated nuclear remodeling in sheep fibroblasts.Palazzese L, Czernik M, Iuso D, Toschi P, Loi P. PLoS One. 2018 Mar 15;13(3):e0193954. doi: 10.1371/journal.pone.0193954. eCollection 2018. IF= 2,766; 
  3. Polychlorinated biphenyls (PCBs) alter DNA methylation and genomic integrity of sheep fetal cells in a simplified in vitro model of pregnancy exposure.Anzalone DA, Sampino S, Czernik M, Iuso D, Ptak GE. Toxicol In Vitro. 2018 Feb;46:39-46. doi: 10.1016/j.tiv.2017.09.017. Epub 2017 Sep 28. IF= 3,105;
  4. Developmental peculiarities in placentae of ovine uniparental conceptuses.Arena R, Zacchini F, Toschi P, Palazzese L, Czernik M, Ptak GE. PLoS One. 2017 Nov 30;12(11):e0188278. doi: 10.1371/journal.pone.0188278. eCollection 2017. IF= 2,766;
  5. Alternative strategies for nuclear reprogramming in somatic cell nuclear transfer (SCNT) Loi P, Iuso D, Toschi P, Palazzese L, Czernik M. Animal Reproduction, Anim Reprod,14, n2, p.377-382, 2017 0,991;
  6. Deregulated Expression of Mitochondrial Proteins Mfn2 and Bcnl3L in Placentae from Sheep Somatic Cell Nuclear Transfer (SCNT) Conceptuses.Czernik M, Toschi P, Zacchini F, Iuso D, Ptak GE. PLoS One. 2017 Jan 11;12(1):e0169579. doi: 10.1371/journal.pone.0169579. eCollection 2017. IF= 2,766;
  7. Evidence of Placental Autophagy during Early Pregnancy after Transfer of In Vitro Produced (IVP) Sheep Embryos.Toschi P, Czernik M, Zacchini F, Fidanza A, Loi P, Ptak GE. PLoS One. 2016 Jun 21;11(6):e0157594. doi: 10.1371/journal.pone.0157594. eCollection 2016. IF= 2,766;
  8. Plasma membrane and acrosome loss before ICSI is required for sheep embryonic development.Anzalone DA, Iuso D, Czernik M, Ptak G, Loi P. J Assist Reprod Genet. 2016 Jun;33(6):757-63. doi: 10.1007/s10815-016-0709-1. Epub 2016 Apr 8. IF= 2,788; 
  9. Impaired placental vasculogenesis compromises the growth of sheep embryos developed in vitro.Fidanza A, Toschi P, Zacchini F, Czernik M, Palmieri C, Scapolo P, Modlinski JA, Loi P, Ptak GE. Biol Reprod. 2014 Jul;91(1):21. doi: 10.1095/biolreprod.113.113902. Epub 2014 May 22. IF= 3,184;
  10. Autophagy and apoptosis: parent-of-origin genome-dependent mechanisms of cellular self-destruction.Ptak GE, Toschi P, Fidanza A, Czernik M, Zacchini F, Modlinski JA, Loi P. Open Biol. 2014 Jun;4(6):140027. doi: 10.1098/rsob.140027. IF= 3, 286;
  11. Towards storage of cells and gametes in dry form.Loi P, Iuso D,Czernik M, Zacchini F, Ptak G. Trends Biotechnol. 2013 Dec;31(12):688-95.  1016/j.tibtech.2013.09.004. Epub 2013 Oct 26. Review.IF= 13,578;
  12. A simplified approach for oocyte enucleation in mammalian cloning.Iuso D, Czernik M, Zacchini F, Ptak G, Loi P. Cell Reprogram. 2013 Dec;15(6):490-4. doi: 10.1089/cell.2013.0051. Epub 2013 Nov 12. IF= 1,43;
  13. Sheep: the first large animal model in nuclear transfer research.Loi P, Czernik M, Zacchini F, Iuso D, Scapolo PA, Ptak G. Cell Reprogram. 2013 Oct;15(5):367-73. doi: 10.1089/cell.2013.0032. Epub 2013 Sep 13. IF= 1,43;
  14. Post-implantation mortality of in vitro produced embryos is associated with DNA methyltransferase 1 dysfunction in sheep placenta.Ptak GE, D'Agostino A, Toschi P, Fidanza A, Zacchini F, Czernik M, Monaco F, Loi P. Hum Reprod. 2013 Feb;28(2):298-305. doi: 10.1093/humrep/des397. Epub 2012 Nov 20. IF= 4,99;
  15. Differentiation potential and GFP labeling of sheep bone marrow-derived mesenchymal stem cells.Czernik M, Fidanza A, Sardi M, Galli C, Brunetti D, Malatesta D, Della Salda L, Matsukawa K, Ptak GE, Loi P. J Cell Biochem. 2013 Jan;114(1):134-43. doi: 10.1002/jcb.24310. IF= 2,959;
  16. Genomic stability of lyophilized sheep somatic cells before and after nuclear transfer.Iuso D, Czernik M, Di Egidio F, Sampino S, Zacchini F, Bochenek M, Smorag Z, Modlinski JA, Ptak G, Loi P. PLoS One. 2013;8(1):e51317. doi: 10.1371/journal.pone.0051317. Epub 2013 Jan 8. IF= 2,766;
  17. A short exposure to polychlorinated biphenyls deregulates cellular autophagy in mammalian blastocyst in vitro.Ptak G, Zacchini F, Czernik M, Fidanza A, Palmieri C, Della Salda L, Scapolo PA, Loi P. Hum Reprod. 2012 Apr;27(4):1034-42. doi: 10.1093/humrep/der454. Epub 2012 Feb 1. IF= 4,99;
  18. Embryonic diapause is conserved across mammals.Ptak GE, Tacconi E, Czernik M, Toschi P, Modlinski JA, Loi P. PLoS One. 2012;7(3):e33027. doi: 10.1371/journal.pone.0033027. Epub 2012 Mar 12. IF= 2,766;
  19. Efficient production and cellular characterization of sheep androgenetic embryos.Zacchini F, Czernik M, Iuso D, Toschi P, di Egidio F, Scapolo PA, Loi P, Ptak G. Cell Reprogram. 2011 Dec;13(6):495-502. doi: 10.1089/cell.2011.0021. Epub 2011 Nov 1.IF= 1,43;
  20. Evidence for phosphatase activity of p27SJ and its impact on the cell cycle.Darbinian N, Czernik M, Darbinyan A, Elias M, Chabriere E, Bonasu S, Khalili K, Amini S. J Cell Biochem. 2009 Jun 1;107(3):400-7. doi: 10.1002/jcb.22135. IF= 2,959;
  21. High levels of anandamide, an endogenous cannabinoid, block the growth of sheep preimplantation embryos by inducing apoptosis and reversible arrest of cell proliferation.Turco MY, Matsukawa K,Czernik M, Gasperi V, Battista N, Della Salda L, Scapolo PA, Loi P, Maccarrone M, Ptak G. Hum Reprod. 2008 Oct;23(10):2331-8. doi: 10.1093/humrep/den258. Epub 2008 Jul 9. IF= 4,99;
  22. John's Wort protein, p27SJ, regulates the MCP-1 promoter.Mukerjee R, Deshmane SL, Darbinian N, Czernik M, Khalili K, Amini S, Sawaya BE. Mol Immunol. 2008 Sep;45(15):4028-35. doi: 10.1016/j.molimm.2008.06.001. Epub 2008 Jul 22. . IF= 3,188;
  23. HIV-1 Tat inhibits NGF-induced Egr-1 transcriptional activity and consequent p35 expression in neural cells.Darbinian N, Darbinyan A, Czernik M, Peruzzi F, Khalili K, Reiss K, Gordon J, Amini S. J Cell Physiol. 2008 Jul;216(1):128-34. doi: 10.1002/jcp.21382. IF= 3,923;
  24. Apigenin inhibits growth and motility but increases gap junctional coupling intensity in rat prostate carcinoma (MAT-LyLu) cell populations.Czernik M, Sroka J, Madeja Z, Czyz J. Cell Mol Biol Lett. 2008;13(3):327-38. doi: 10.2478/s11658-008-0003-z. Epub 2008 Feb 21. IF= 1,291;
  25. Dysregulation of NGF-signaling and Egr-1 expression by Tat in neuronal cell culture.Darbinian-Sarkissian N, Czernik M, Peruzzi F, Gordon J, Rappaport J, Reiss K, Khalili K, Amini S. J Cell Physiol. 2006 Sep;208(3):506-15. IF= 3,923;
  26. Contact stimulation of prostate cancer cell migration: the role of gap junctional coupling and migration stimulated by heterotypic cell-to-cell contacts in determination of the metastatic phenotype of Dunning rat prostate cancer cells.Miekus K, Czernik M, Sroka J, Czyz J, Madeja Z. Biol Cell. 2005 Dec;97(12):893-903. IF= 3,226;
  27. Sheep as an Experimental Model for Human ART: Novel Insights on Phenotypic Alterations in ART-Derived Sheep Conceptuses Loi, P.,D'Agostino, A.Czernik M, Zacchini, F., Toschi, P.,Fidanza, A.,Ptak, G.Livestock Epigenetics 59-71 (book chapter)
  28. Iuso D, Czernik M*,Toschi P, Fidanza A, Zacchini F, Feil R, Curtet S, Buchou T, Shiota H, Khochbin S, Ptak GE, Loi P. Exogenous Expression of Human Protamine 1 (hPrm1) Remodels Fibroblast Nuclei into Spermatid-like Structures.Cell Rep. 2015 Dec 1;13(9):1765-71 *pierwszy autor /równy wkład IF= 8,282,
  29. Loi P, Iuso D, Czernik M,Ogura A. A New, Dynamic Era for Somatic Cell Nuclear Transfer?Trends Biotechnol. 2016 Oct;34(10):791-797 IF= 13,578
  30. Czernik M, Iuso D, Toschi P, Khochbin S, Loi P.Remodeling somatic nuclei via exogenous expression of protamine 1 to create spermatid-like structures for somatic nuclear transfer.Nat Protoc. 2016 Nov;11(11):2170-2188 IF= 12,423.



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Ultimo aggiornamento: 06-02-2024