Prof. LOI Pasqualino
 
 

Curriculum accademico

 

 

Family name:  Loi First name:Pasqualino

ORCID: 0000-0003-4631-7663 Date of birth: 15th October 1958

EDUCATION

1991 PhD in Physiology of Reproduction, University of Sassari, Italy

1986 Degree in Veterinary Medicine, University of Sassari, Italy

CURRENT POSITION(S) 2019 January-present: Full Professor, Faculty of Veterinary, University of Teramo, Italy    PREVIOUS POSITIONS 2000 - 2018: Associate Professor, Faculty of Veterinary, University of Teramo, Italy 1994 - 2000 October: Senior Scientist, Institute of Zootechnic, Sardinia, Italy

1992 - 1994 Post Doc; Roslin Institute, Edinburgh, Scotland, UK

    FELLOWSHIPS AND AWARDS

1994 EMBO Fellow, University of Newcastle upon Tyne, UK

2017 Japanese Society for the Promotion of Science (JSPS) Fellow, Riken Institute, Tsukuba, Japan

    SUPERVISION OF GRADUATE STUDENTS AND POSTDOCTORAL FELLOWS

Under graduate students of Veterinary Sciences and Biotechnology of Reproduction (n° 23); PhD students (n°15); Post Docs: (n° 9)

2005 External Evaluator of the PhD program in the Polish Academy of Science, Poland 2008

External Evaluator of the PhD program in Nottingham University, UK

2017 External Evaluator of the PhD program in Bologna University, Italy 2016 External Evaluator of the PhD program in Milan University, Italy;

ORGANISATION OF SCIENTIFIC MEETINGS (last 5 years)

Member of the Scientific and Organizing Committee: International Congress on Animal Reproduction (ICAR), Bologna, June 2022, Italy

Member of the Scientific and Organizing Committee; Workshop "Micromanipulation of oocyte/embryos”, 33rd Meeting of the Association of Embryo Technologies in Europe, Bath, 8-9 September 2017; UK

Member of the Scientific and Organizing Committee; International Conference “Epigenetic Penetrance of Reproductive Technologies”, University of Teramo, 9-10 June 2016, project EU Horizon2020 "EraofArt", Twinning Action.

INSTITUTIONAL RESPONSIBILITIES

2009 - 2011: Scientific Advisory Board, EU ANIMBIOGEN, VII FP EU, Polish Academy of Sciences, Warsaw, Poland.

2007 - present: Review Board member for the European Science Foundation 2008-2011: Deputy of the EU COST “Gemini” Managing Committee –EU VII FP.

2004 - Present: PhD Committee in Veterinary Sciences, University of Teramo

2012 - Present: Member of the Steering Committee for the European Association of European Veterinary Education (EAVE)

2018 - present: Responsible person for students with disabilities of the Faculty of Veterinary Medicine in Teramo

2019 - present: President of the Committee for the Research Assessment, Faculty of Veterinary, University of Teramo.

2020 – present: European Research Council, Executive Agency (ERCEA), Seconded National Experts, Department B, Life Sciences, reserve list.

 

Editorial Board Member

 Animal Science Papers and Reports. ISSN: 0860 4037

Cellular Reprogramming. ISSN: 2152- 4971                                          

   Journal of Reproduction and Development”. ISSN: 1348-4400

   Frontiers in Cell and Developmental Biology: Electronic    ISSN: 2296-634X

   Frontiers in Physiology: Electronic ISSN: 1664-042X

 

Review, ad hoc

Ad hoc reviewer: Nature, Biology of Reproduction, Theriogenology, Gene, Trends in Biotechnology, Cloning and Stem Cells, Reproduction, Fertility and Development, Genomics, Reproduction in Domestic Animals, PLOS ONE, Scientific Reports, etc.

    Referee for funding agencies

    2007 –Referee for the European Research Council –remote evaluation - (ERC), VII FP EU

    2010-2014 Referee for the Marie Curie Fellowship Program, VII FP EU

    2011-2019 Referee for the Polish Academy of Sciences

    2013 Referee for the Royal Society, UK

    2013 Referee for the USA Israel Bi-National Scientific Program

    2015-2019 Referee for the Cyprus Academy of Sciences

    2017-to present Referee for the European Science Foundation

    2018 Referee for the Lithuanian National Science Foundation

    2018 Referee for the Medical Research Council, UK

    2019 Referee for the Fonds Wetenschappelijk Onderzoek, Belgium

    2018-to present Referee for the Dutch Science Foundation

    2018-to present Referee for the Czech Academy of Sciences

    2010-to present Referee for the Italian Minister of Research and University

    2020-to present Referee for the Natural Sciences and Engineering Research Council of Canada.

  MEMBERSHIPS OF SCIENTIFIC SOCIETIES

Italian Society of Veterinary Sciences (SISVET); Italian Association of Veterinary Physiologists,   Steering Committee (SOFIVET; International Embryo Technology Association (IETS)

Granted Patents

  1. Pasqualino Loi et al., Improved method for reconstructing a non-human animal embryo EP 3134532 A1 (WO2015162170A1)
  2. Pasqualino Loi & Pietro Cappai. Process for reconstructing a non-human mammalian embryo by nuclear transfer using a heat-treated donor nucleus and preparing a non-human mammal US 7064245 B1; PCT/IT1999/000160

Invited presentations to internationally established conferences (last 5 years):

  1. 2021 International Embryo Technology Society (IETS) 47th Annual Conference, Lima,
  2. 2020 Ovarian Club, XIV Symposium, Paris,
  3. 2019 Ovarian Club, XIV Symposium, Paris,
  4. 2018 Ovarian Club, XIII Symposium, Paris,
  5. 2017 World Congress of Reproduction Biology (WCRB2017), Okinawa,
  6. 2017 Laudazio Galli: recipient of the AETE pioneer award 2017", 33rd Meeting of the Association of Embryo Technology in Europe (AETE). Bath, UK.
  7. 2016 VI International Symposium of Animal Biology of Reproduction. Campos do Jordao, SP, Brazil.
  8. 2015 International Northern White Rhino Working Group: Conservation by Cellular Technologies, Vienna,
  9. 2015 Cost Action Meeting Epigenetics and Peri-conception Environment, Hersonissos, Crete, Greece.
  10. 2015 International Symposium of Functional Genomics Piacenza, Piacenza,
  11. 2015 International Embryo Technology Society (IETS) 41st Annual Conference, Versailles,

Short Biographical Sketch

I started to work on embryo micromanipulation, 30 years ago on embryo multiplication by splitting and embryonic cells nuclear transfer during my PhD. Next I joined Keith Campbell and Ian Wilmut at the Roslin Institute, UK, as a Post Doc. The publications jointly delivered (Campbell, KHS Loi et al., Reviews of Reproduction, 1996; Campbell KHS, Loi P., Biol Reprod. 1994) prepared the ground for the production of the first sheep cloned from embryonic (Campbell et al., Nature 1996;380: 64) and adult cells (Wilmut et al., Nature 1997, 385: 810). After my work at Roslin, I continued to work on Somatic Cell Nuclear Transfer (SCNT), producing the first wild animal by cloning (Loi et al. Nature Biotechnology, 2001), even using dead cells (Loi et al., Biol Reprod. 2002), a breakthrough that has prepared the ground for cloning using lyophilized cells (Loi et al., PLOS ONE 2008), and paved the way to the most recent attempt to clone the mammoth (Yamagata et al., Sci Rep. 2019; 9:4050). The latest breakthrough of my research group is to improve nuclear reprogramming by conferring somatic cells the nuclear structure of a spermatid through the heterologous expression of protamine1 (Iuso et al., Cell Reports 2015; Czernik et al., Nature Protocols 2016; Palazzese et al., PLOS ONE, 2018).

Metrics - My H Index is 36 (Google Scholar), with 4450 citations. My H- index and number of citations may appear low; however, I was amongst the first researchers to work on nuclear transfer. For many years the number of groups worldwide on the topics was below 10 so it is not possible to have a high H-index in such circumstances, considering also that I publish on animal science, notoriously with low IF.

Latest Dissemination as influential Scientist in my field https://www.nature.com/news/endangered-species-sex-and-the-single-rhinoceros-1.10731 https://www.scientificamerican.com/article/scientists-successfully-c/ https://www.scientificamerican.com/article/spry-dolly-siblings-could-make-clone-skeptics-sheepishhttps://www.scientificamerican.com/article/cloning-endangered-animals/https://www.scientificamerican.com/article/rhinoceros-assisted-reproduction-rescue-sepcies  http://www.nytimes.com/2016/07/27/science/dolly-the-sheep-clones.html?_r=0; https://doi.org/10.1038/nature.2012.10228

Curriculum english version

 

Family name:  Loi First name:Pasqualino

ORCID: 0000-0003-4631-7663 Date of birth: 15th October 1958

EDUCATION

1991 PhD in Physiology of Reproduction, University of Sassari, Italy

1986 Degree in Veterinary Medicine, University of Sassari, Italy

CURRENT POSITION(S) 2019 January-present: Full Professor, Faculty of Veterinary, University of Teramo, Italy    PREVIOUS POSITIONS 2000 - 2018: Associate Professor, Faculty of Veterinary, University of Teramo, Italy 1994 - 2000 October: Senior Scientist, Institute of Zootechnic, Sardinia, Italy

1992 - 1994 Post Doc; Roslin Institute, Edinburgh, Scotland, UK

    FELLOWSHIPS AND AWARDS

1994 EMBO Fellow, University of Newcastle upon Tyne, UK

2017 Japanese Society for the Promotion of Science (JSPS) Fellow, Riken Institute, Tsukuba, Japan

    SUPERVISION OF GRADUATE STUDENTS AND POSTDOCTORAL FELLOWS

Under graduate students of Veterinary Sciences and Biotechnology of Reproduction (n° 23); PhD students (n°15); Post Docs: (n° 9)

2005 External Evaluator of the PhD program in the Polish Academy of Science, Poland 2008

External Evaluator of the PhD program in Nottingham University, UK

2017 External Evaluator of the PhD program in Bologna University, Italy 2016 External Evaluator of the PhD program in Milan University, Italy;

ORGANISATION OF SCIENTIFIC MEETINGS (last 5 years)

Member of the Scientific and Organizing Committee: International Congress on Animal Reproduction (ICAR), Bologna, June 2022, Italy

Member of the Scientific and Organizing Committee; Workshop "Micromanipulation of oocyte/embryos”, 33rd Meeting of the Association of Embryo Technologies in Europe, Bath, 8-9 September 2017; UK

Member of the Scientific and Organizing Committee; International Conference “Epigenetic Penetrance of Reproductive Technologies”, University of Teramo, 9-10 June 2016, project EU Horizon2020 "EraofArt", Twinning Action.

INSTITUTIONAL RESPONSIBILITIES

2009 - 2011: Scientific Advisory Board, EU ANIMBIOGEN, VII FP EU, Polish Academy of Sciences, Warsaw, Poland.

2007 - present: Review Board member for the European Science Foundation 2008-2011: Deputy of the EU COST “Gemini” Managing Committee –EU VII FP.

2004 - Present: PhD Committee in Veterinary Sciences, University of Teramo

2012 - Present: Member of the Steering Committee for the European Association of European Veterinary Education (EAVE)

2018 - present: Responsible person for students with disabilities of the Faculty of Veterinary Medicine in Teramo

2019 - present: President of the Committee for the Research Assessment, Faculty of Veterinary, University of Teramo.

2020 – present: European Research Council, Executive Agency (ERCEA), Seconded National Experts, Department B, Life Sciences, reserve list.

 

Editorial Board Member

 Animal Science Papers and Reports. ISSN: 0860 4037

Cellular Reprogramming. ISSN: 2152- 4971                                          

   Journal of Reproduction and Development”. ISSN: 1348-4400

   Frontiers in Cell and Developmental Biology: Electronic    ISSN: 2296-634X

   Frontiers in Physiology: Electronic ISSN: 1664-042X

 

Review, ad hoc

Ad hoc reviewer: Nature, Biology of Reproduction, Theriogenology, Gene, Trends in Biotechnology, Cloning and Stem Cells, Reproduction, Fertility and Development, Genomics, Reproduction in Domestic Animals, PLOS ONE, Scientific Reports, etc.

    Referee for funding agencies

    2007 –Referee for the European Research Council –remote evaluation - (ERC), VII FP EU

    2010-2014 Referee for the Marie Curie Fellowship Program, VII FP EU

    2011-2019 Referee for the Polish Academy of Sciences

    2013 Referee for the Royal Society, UK

    2013 Referee for the USA Israel Bi-National Scientific Program

    2015-2019 Referee for the Cyprus Academy of Sciences

    2017-to present Referee for the European Science Foundation

    2018 Referee for the Lithuanian National Science Foundation

    2018 Referee for the Medical Research Council, UK

    2019 Referee for the Fonds Wetenschappelijk Onderzoek, Belgium

    2018-to present Referee for the Dutch Science Foundation

    2018-to present Referee for the Czech Academy of Sciences

    2010-to present Referee for the Italian Minister of Research and University

    2020-to present Referee for the Natural Sciences and Engineering Research Council of Canada.

  MEMBERSHIPS OF SCIENTIFIC SOCIETIES

Italian Society of Veterinary Sciences (SISVET); Italian Association of Veterinary Physiologists,   Steering Committee (SOFIVET; International Embryo Technology Association (IETS)

Granted Patents

  1. Pasqualino Loi et al., Improved method for reconstructing a non-human animal embryo EP 3134532 A1 (WO2015162170A1)
  2. Pasqualino Loi & Pietro Cappai. Process for reconstructing a non-human mammalian embryo by nuclear transfer using a heat-treated donor nucleus and preparing a non-human mammal US 7064245 B1; PCT/IT1999/000160

Invited presentations to internationally established conferences (last 5 years):

  1. 2021 International Embryo Technology Society (IETS) 47th Annual Conference, Lima,
  2. 2020 Ovarian Club, XIV Symposium, Paris,
  3. 2019 Ovarian Club, XIV Symposium, Paris,
  4. 2018 Ovarian Club, XIII Symposium, Paris,
  5. 2017 World Congress of Reproduction Biology (WCRB2017), Okinawa,
  6. 2017 Laudazio Galli: recipient of the AETE pioneer award 2017", 33rd Meeting of the Association of Embryo Technology in Europe (AETE). Bath, UK.
  7. 2016 VI International Symposium of Animal Biology of Reproduction. Campos do Jordao, SP, Brazil.
  8. 2015 International Northern White Rhino Working Group: Conservation by Cellular Technologies, Vienna,
  9. 2015 Cost Action Meeting Epigenetics and Peri-conception Environment, Hersonissos, Crete, Greece.
  10. 2015 International Symposium of Functional Genomics Piacenza, Piacenza,
  11. 2015 International Embryo Technology Society (IETS) 41st Annual Conference, Versailles,

Short Biographical Sketch

I started to work on embryo micromanipulation, 30 years ago on embryo multiplication by splitting and embryonic cells nuclear transfer during my PhD. Next I joined Keith Campbell and Ian Wilmut at the Roslin Institute, UK, as a Post Doc. The publications jointly delivered (Campbell, KHS Loi et al., Reviews of Reproduction, 1996; Campbell KHS, Loi P., Biol Reprod. 1994) prepared the ground for the production of the first sheep cloned from embryonic (Campbell et al., Nature 1996;380: 64) and adult cells (Wilmut et al., Nature 1997, 385: 810). After my work at Roslin, I continued to work on Somatic Cell Nuclear Transfer (SCNT), producing the first wild animal by cloning (Loi et al. Nature Biotechnology, 2001), even using dead cells (Loi et al., Biol Reprod. 2002), a breakthrough that has prepared the ground for cloning using lyophilized cells (Loi et al., PLOS ONE 2008), and paved the way to the most recent attempt to clone the mammoth (Yamagata et al., Sci Rep. 2019; 9:4050). The latest breakthrough of my research group is to improve nuclear reprogramming by conferring somatic cells the nuclear structure of a spermatid through the heterologous expression of protamine1 (Iuso et al., Cell Reports 2015; Czernik et al., Nature Protocols 2016; Palazzese et al., PLOS ONE, 2018).

Metrics - My H Index is 35 (Google Scholar), with 4250 citations. My H- index and number of citations may appear low; however, I was amongst the first researchers to work on nuclear transfer. For many years the number of groups worldwide on the topics was below 10 so it is not possible to have a high H-index in such circumstances, considering also that I publish on animal science, notoriously with low IF.

Latest Dissemination as influential Scientist in my field https://www.nature.com/news/endangered-species-sex-and-the-single-rhinoceros-1.10731 https://www.scientificamerican.com/article/scientists-successfully-c/ https://www.scientificamerican.com/article/spry-dolly-siblings-could-make-clone-skeptics-sheepishhttps://www.scientificamerican.com/article/cloning-endangered-animals/https://www.scientificamerican.com/article/rhinoceros-assisted-reproduction-rescue-sepcies  http://www.nytimes.com/2016/07/27/science/dolly-the-sheep-clones.html?_r=0; https://doi.org/10.1038/nature.2012.10228

Pubblicazioni

 
  1. Czernik M, Fidanza A, Luongo FP, Valbonetti L, Scapolo PA, Patrizio P,Loi P. Late Embryogenesis Abundant (LEA) proteins confer water stress tolerance to mammalian somatic cells. Cryobiology. 2020 Jan 14. pii: S0011-2240(19)30232-9

 

  1. Helena Fulka, Jana Rychtarova & Pasqualino Loi. The nucleolus-like and precursor bodies of mammalian oocytes and 1 embryos and their possible role in post-fertilisation centromere remodelling.  Biochemical Society Transactions, 2020, in press

 

  1. Saragusty J, Anzalone DA, Palazzese L, Arav A, Patrizio P, Gosálvez J,Loi P.  Dry biobanking as a conservation tool in the Anthropocene. Theriogenology. 2020 Jan 13. pii: S0093-691X(20)30028-5.

 

  1. Toschi P, Capra E, Anzalone DA, Lazzari B, Turri F, Pizzi F, Scapolo PA, Stella A, Williams JL, Ajmone Marsan P,Loi P. Maternal peri-conceptional undernourishment perturbs offspring sperm methylome. Reproduction. 2020 May;159(5):513-523

 

1. Saragusty J, Loi P (2019) Exploring dry storage as an alternative biobanking strategy inspired by Nature. Theriogenology 126:17-27. doi: 10.1016/j.theriogenology.2018.11.027

*2. Anzalone DA, Palazzese L, Iuso D, Martino G, Loi P (2018) Freeze-dried spermatozoa: An alternative biobanking option for endangered species. Anim Reprod Sci 190:85-93. doi: 10.1016/j.anireprosci.2018.01.010

3. Hildebrandt TB, Hermes R, Colleoni S, Diecke S, Holtze S, Renfree MB, Stejskal J, Hayashi K, Drukker M, Loi P, Göritz F, Lazzari G, Galli C (2018) Embryos and embryonic stem cells from the white rhinoceros. Nat Commun 9 (1):2589. doi: 10.1038/s41467-018-04959-2

4. Loi P, Galli C, Lazzari G, Matsukawa K, Fulka Jr J, Goeritz F, Hildebrandt TB (2018) Development to term of sheep embryos reconstructed after inner cell mass/trophoblast exchange. J Reprod Dev 64 (2):187-191. doi: 10.1262/jrd.2017-109

*5. Palazzese L, Czernik M, Iuso D, Toschi P, Loi P (2018) Nuclear quiescence and histone hyper-acetylation jointly improve protamine-mediated nuclear remodeling in sheep fibroblasts. PLOS ONE 13 (3):e0193954. doi: 10.1371/journal.pone.0193954

*6. Palazzese L, Gosálvez J, Anzalone DA, Loi P, Saragusty J (2018) DNA fragmentation in epididymal freeze-dried ram spermatozoa impairs embryo development. J Reprod Dev 64 (5):393-400. doi: 10.1262/jrd.2018-033

7. Capra E, Toschi P, Del Corvo M, Lazzari B, Scapolo PA, Loi P, Williams JL, Stella A, Ajmone-Marsan P (2017) Genome-wide epigenetic characterization of tissues from three germ layers isolated from sheep fetuses. Front Genet 8:Article 115. doi: 10.3389/fgene.2017.00115

8. Loi P, Iuso D, Toschi P, Palazzese L, Czernik M (2017) Alternative strategies for nuclear reprogramming in somatic cell nuclear transfer (SCNT). Anim Reprod 14 (2):377-382. doi: 10.21451/1984-3143-AR915

9. Anzalone DA, Iuso D, Czernik M, Ptak G, Loi P (2016) Plasma membrane and acrosome loss before ICSI is required for sheep embryonic development. J Assist Reprod Genet 33 (6):757-763. doi: 10.1007/s10815-016-0709-1

*10. Czernik M, Iuso D, Toschi P, Khochbin S, Loi P (2016) Remodeling somatic nuclei via exogenous expression of protamine 1 to create spermatid-like structures for somatic nuclear transfer. Nat Protoc 11 (11):2170-2188. doi: 10.1038/nprot.2016.130

11. Loi P, Iuso D, Czernik M, Ogura A (2016) A new, dynamic era for somatic cell nuclear transfer? Trends Biotech 34 (10):791-797. doi: 10.1016/j.tibtech.2016.03.008

12. Loi P, Toschi P, Zacchini F, Ptak G, Scapolo PA, Capra E, Stella A, Marsan PA, Williams JL (2016) Synergies between assisted reproduction technologies and functional genomics. Genetics Selection Evolution 48 (1):53. doi: 10.1186/s12711-016-0231-z

13. Saragusty J, Diecke S, Drukker M, Durrant B, Friedrich Ben-Nun I, Galli C, Göritz F, Hayashi K, Hermes R, Holtze S, Johnson S, Lazzari G, Loi P, Loring JF, Okita K, Renfree MB, Seet S, Voracek T, Stejskal J, Ryder OA, Hildebrandt TB (2016) Rewinding the process of mammalian extinction. Zoo Biol 35 (4):280-292. doi: 10.1002/zoo.21284

14. Toschi P, Czernik M, Zacchini F, Fidanza A, Loi P, Ptak GE (2016) Evidence of placental autophagy during early pregnancy after transfer of in vitro produced (IVP) sheep embryos. PLOS ONE 11 (6):e0157594. doi: 10.1371/journal.pone.0157594

15. Cree L, Loi P (2015) Mitochondrial replacement: From basic research to assisted reproductive technology portfolio tool-technicalities and possible risks. Molecular Human Reproduction 21 (1):3-10. doi: 10.1093/molehr/gau082

*16. Iuso D, Czernik M, Toschi P, Fidanza A, Zacchini F, Feil R, Curtet S, Buchou T, Shiota H, Khochbin S, Ptak Grazyna E, Loi P (2015) Exogenous expression of human protamine 1 (hPrm1) remodels fibroblast nuclei into spermatid-like structures. Cell Reports 13 (9):1765-1771. doi: 10.1016/j.celrep.2015.10.066

17. Cree L, Loi P (2014) Mitochondrial replacement: From basic research to assisted reproductive technology portfolio tool—technicalities and possible risks. Molecular Human Reproduction 21 (1):3-10. doi: 10.1093/molehr/gau082

18. Fidanza A, Toschi P, Zacchini F, Czernik M, Palmieri C, Scapolo P, Modlinski JA, Loi P, Ptak GE (2014) Impaired placental vasculogenesis compromises the growth of sheep embryos developed in vitro. Biol Reprod 91 (1):Article 21, 21-27. doi: 10.1095/biolreprod.113.113902

19. Loi P, Saragusty J, Ptak G (2014) Cloning the mammoth: A complicated task or just a dream? In: Holt WV, Brown JL, Comizzoli P (eds) Reproductive Sciences in Animal Conservation, vol 753. Advances in Experimental Medicine and Biology. Springer New York, pp 489-502. doi: 10.1007/978-1-4939-0820-2_19

20. Ptak GE, Toschi P, Fidanza A, Czernik M, Zacchini F, Modlinski JA, Loi P (2014) Autophagy and apoptosis: Parent-of-origin genome-dependent mechanisms of cellular self-destruction. Open Biology 4 (6):140027. doi: 10.1098/rsob.140027

21. Sampino S, Juszczak GR, Zacchini F, Swiergiel AH, Modlinski JA, Loi P, Ptak GE (2014) Grand-paternal age and the development of autism-like symptoms in mice progeny. Transl Psychiatry 4:e386. doi: 10.1038/tp.2014.27

22. Zacchini F, Loi P, Sampino S, Ptak GE, Swiergiel AH, Modlinski AJ (2014) Effects of blastomere biopsy on post-natal growth and behavior in mice. Hum Reprod 29 (9):1875-1883. doi: 10.1093/humrep/deu145

Loi P, Czernik M, Zacchini F, Iuso D, Scapolo PA, Ptak G (in stampa). Sheep: The First Large Animal Model in Nuclear Transfer Research. CELLULAR REPROGRAMMING, ISSN: 2152-4971

S Sampino, G R Juszczak, F Zacchini, A H Swiergiel, J A Modlinski, P Loi, G E Ptak (2014). Grand-paternal age and the development of autism-like symptoms in mice progeny. TRANSLATIONAL PSYCHIATRY, vol. 4, ISSN: 2158-3188, doi: 10.1038/tp.2014.27

Iuso D, Czernik M, Zacchini F, Ptak G, Loi P (2013). A simplified approach for oocyte enucleation in mammalian cloning.. CELLULAR REPROGRAMMING, vol. 15, ISSN: 2152-4971, doi: 10.1089/cell.2013.0051

Czernik M, Fidanza A, Sardi M, Galli C, Brunetti D, Malatesta D, Della Salda L, Matsukawa K, Ptak G, Loi P (2013). Differentiation potential and GFP labeling of sheep bone marrow derived mesenchymal stem cells.. JOURNAL OF CELLULAR BIOCHEMISTRY, vol. 114, p. 134-143, ISSN: 0730-2312, doi: 10.1002/jcb.24310.

 

Ptak GE, Modlinski JA, Loi P (2013). Embryonic diapause in humans: time to consider?. REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, vol. 11, ISSN: 1477-7827, doi: 10.1186/1477-7827-11-92

 

 

Iuso D, Czernik M, Di Egidio F, Sampino S, Zacchini F, Bochenek M, Smorag Z, Modlinski JA, Ptak G, Loi P (2013). Genomic stability of lyophilized sheep somatic cells before and after nuclear transfer. PLOS ONE, vol. Epub 2013 Jan 8., p. 1-10, ISSN: 1932-6203, doi: 10.1371/journal.pone.0051317

 

Iuso D, Czernik M, Di Egidio F, Sampino S, Zacchini F, Bochenek M, Smorag Z, Modlinski JA, Ptak G, Loi P (2013). Genomic stability of lyophilized sheep somatic cells before and after nuclear transfer.. PLOS ONE, vol. 8, ISSN: 1932-6203, doi: 10.1371/journal.pone.0051317

 

 

Ptak GE, D'Agostino A, Toschi P, Fidanza A, Zacchini F, Czernik M, Monaco F, Loi P (2013). Post-implantation mortality of in vitro produced embryos is associated with DNA methyltransferase 1 dysfunction in sheep placenta. HUMAN REPRODUCTION, vol. 28, p. 298-305, ISSN: 0268-1161, doi: 10.1093/humrep/des397

 

 

Ptak GE, D'Agostino A, Toschi P, Fidanza A, Zacchini F, Czernik M, Monaco F, Loi P (2013). Post-implantation mortality of in vitro produced embryos is associated with DNA methyltransferase 1 dysfunction in sheep placenta.. HUMAN REPRODUCTION, vol. 28, ISSN: 0268-1161, doi: 10.1093/humrep/des397

 

 

Ptak G, D'Agostino Antonella, Toschi Paola, Fidanza Antonella, Zacchini Federica, Marta Czernik, Monaco Federica, Loi Pasqualino (2013). Postimplantational mortality of in vitro produced embryos is associated with DNA methyltransferase 1 (DNMT1) dysfunction in placenta.. HUMAN REPRODUCTION, ISSN: 0268-1161, doi: 10.1093/humrep/des397

allegati

 

Loi P, Czernik M, Zacchini F, Iuso D, Scapolo PA, Ptak G (2013). Sheep: the first large animal model in nuclear transfer research.. CELLULAR REPROGRAMMING, vol. 15, ISSN: 2152-4971, doi: 10.1089/cell.2013.0032

 

 

Loi P., Czernik M., Zacchini F., Iuso D., Scapolo P.A., Ptak G. (2013). Sheep: the first large model in nuclear transfer research. HUMAN REPRODUCTION, vol. 15, p. 367-373, ISSN: 0268-1161, doi: 10.1089/cell.2013.0032

 

 

Fulka J Jr, Langerova A, Loi P, Ptak G, Albertini D, Fulka H (2013). The ups and downs of somatic cell nucleus transfer (SCNT) in humans.. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, vol. 30, ISSN: 1058-0468, doi: 10.1007/s10815-013-0053-7

 

 

Ptak G, Zacchini F, Czernik M, Fidanza A, Palmieri C, Della Salda L, Scapolo PA, Loi P (2012). A short exposure to polychlorinated biphenyls deregulates cellular autophagy in mammalian blastocyst in vitro.. HUMAN REPRODUCTION, vol. 27, p. 1034-1042, ISSN: 0268-1161, doi: 10.1093/humrep/der454

allegati

 

Ptak G, Tacconi E, Czernik M, Toschi P, Modlinski JA, Loi P (2012). Embryonic diapause is conserved across mammals.. PLOS ONE, vol. 7, p. 1-7, ISSN: 1932-6203, doi: 10.1186/1477-7827-11-92

 

 

Loi P, Teruhiko Wakayama, Josef Fulka Jr and Grazyna Ptak. (2011). Biological time machines: a realistic approach for cloning an extinct mammal.. ENDANGERED SPECIES RESEARCH, vol. 14, p. 227-233, ISSN: 1613-4796

Zacchini F, Czernik M, Iuso D, Toschi P, di Egidio F, Scapolo P.A., Loi P, Ptak G (2011). Efficient Production and Cellular Characterization of Sheep Androgenetic Embryos. CELLULAR REPROGRAMMING, vol. Dec;13(6), p. 495-502, ISSN: 2152-4971, doi: 10.1089/cell.2011.0021

Loi P, Fulka J Jr, Hildebrand T, Ptak G (2011). Genome of non-living cells: trash or recycle?. REPRODUCTION, vol. 76, p. 217-228, ISSN: 1470-1626

Loi P, Modlinski JA, Ptak G (2011). Interspecies somatic cell nuclear transfer: a salvage tool seeking first aid.. THERIOGENOLOGY, vol. 76, p. 217-228, ISSN: 0093-691X

Fulka J Jr, Langerova A, Loi P, Martinkova S, Fulka H. (2011). Transplantation of nucleoli into human zygotes: not as simple as expected? . JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, vol. 28(5), p. 385, ISSN: 1058-0468

FULKA JR J, LOI P, PTAK G, FULKA H, JOHN JS (2009). Hope for the Mammoth?. CLONING AND STEM CELLS, ISSN: 1536-2302

 

 

LOI P, BEAUJEAN N, KHOCHBIN S, FULKA J JR, PTAK G (2008). Asymmetric nuclear reprogramming in somatic cell nuclear transfer?. BIOESSAYS, vol. 30, p. 66-74, ISSN: 0265-9247

 

 

LOI P, GALLI C, PTAK G (2008). Cloning of endangered mammalian species: any progress?. TRENDS IN BIOTECHNOLOGY, vol. 25, p. 195-200, ISSN: 0167-7799

 

 

FULKA J JR, FULKA H, ST JOHN J, GALLI C, LAZZARI G, LAGUTINA I, FULKA J, LOI P (2008). Cybrid human embryos--warranting opportunities to augment embryonic stem cell research. TRENDS IN BIOTECHNOLOGY, vol. 26, p. 469-474, ISSN: 0167-7799

 

 

LOI P, MATSUKAWA K, PTAK G, CLINTON M, FULKA J JR, NATHAN Y, ARAV A (2008). Freeze-dried somatic cells direct embryonic development after nuclear transfer. PLOS ONE, vol. e2978., ISSN: 1932-6203

 

 

TURCO MY, MATSUKAWA K, CZERNIK M, GASPERI V, BATTISTA N, DELLA SALDA L, SCAPOLO P.A., LOI P, MACCARRONE M, PTAK G. HUMAN REPROD. OCT, - (2008). High levels of anandamide, an endogenous cannabinoid, block the growth of sheep preimplantation embryos by inducing apoptosis and reversible arrest of cell proliferation. HUMAN REPRODUCTION, vol. 23, p. 2331-2338, ISSN: 0268-1161, doi: 10.1093/humrep/den258

 

 

Loi P, Matsukawa K, Takahashi S, Akagi S, Ptak G (2008). Nuclear reprogramming: what has been done and potential avenues for improvements. CENTRAL EUROPEAN JOURNAL OF BIOLOGY, vol. 3, p. 211-223, ISSN: 1895-104X

 

 

LOI P, MATZUKAWA K, PTAK G, NATAN Y, FULKA J JR, ARAV A (2008). Nuclear transfer of freeze-dried somatic cells into enucleated sheep oocytes. REPRODUCTION IN DOMESTIC ANIMALS, vol. 43, p. 417-422, ISSN: 0936-6768

 

 

PALMIERI C, LOI P, PTAK G, DELLA SALDA L (2008). Review paper: a review of the pathology of abnormal placentae of somatic cell nuclear transfer clone pregnancies in cattle, sheep, and mice. VETERINARY PATHOLOGY, vol. 45, p. 865-880, ISSN: 0300-9858


LOI P, GALLI C, PTAK G (2007). Cloning of endangered mammalian species: any progress?. TRENDS IN BIOTECHNOLOGY, vol. 25, p. 195-200, ISSN: 0167-7799

Matsukawa K, Turco MY, Scapolo PA, Reynolds L, Ptak G, Loi P (2007). Development of sheep androgenetic embryos is boosted following transfer of male pronuclei into androgenetic hemizygotes. . CLONING AND STEM CELLS, vol. 9, p. 374-381, ISSN: 1536-2302, doi: 10.1089/clo.2006.0016

PALMIERI C, LOI P, REYNOLDS LP, PTAK G, DELLA SALDA (2007). Placental abnormalities in ovine somatic cell clones at term: a light and electron microscopic investigation. PLACENTA, vol. 28, p. 577-584, ISSN: 0143-4004, doi: 10.1016/j.placenta.2006.08.003

PTAK G, MATSUKAWA K, PALMIERI C, DELLA SALDA L, SCAPOLO PA, LOI P (2006). Developmental and functional evidence of nuclear immaturity in prepubertal oocytes. HUMAN REPRODUCTION, vol. 21 (9), p. 2228-2237, ISSN: 0268-1161, doi: 10.1093/humrep/del184

 

 

PTAK G, LOPES F., MATSUKAWA K., TISCHNER M., LOI P. (2006). Leukaemia inhibitory factor enhances sheep fertilization in vitro via an influence on the oocyte. THERIOGENOLOGY, vol. 65, p. 1891-1899, ISSN: 0093-691X

 

 

 

GRAZYNA PTAK, LOI P, FEDERICA LOPES, KAZUTSUGU MATSUKAWA, MARIAN TISCHNER, PASQUALINO LOI (2006). Leukemia Inhibitory Factor enhances sheep ferilization in vitro via an influence on the oocyte. THERIOGENOLOGY, p. 1-10, ISSN: 0093-691X

 

 

LOI P., CLINTON M., VACKOVA I., FULKA JR., J., FEIL R., PALMIERI, DELLA SALDA L., PTAK G (2006). Placental abnormalities associated with post-natal mortality in sheep somatic cell clones. THERIOGENOLOGY, vol. 65, p. 1110-1121, ISSN: 0093-691X, doi: 10.1016/j.theriogenology.2005.07.016

 

 

PASQUALINO LOI, MICHAEL CLINTON, IRENA VACKOVA, JOSEF FULKA JR, ROBERT FEIL, CHIARA PALMIERI, LEONARDO DELLA SALDA, PTAK G (2006). Placental abnormalities associated with post-natal mortality in sheep somatic cell clones. THERIOGENOLOGY, vol. 65, p. 1110-1121, ISSN: 0093-691X, doi: 10.1016/j.theriogenology.2005.07.016

 

 

IRENA VACKOVA, RADOMIR KREN, PASQUALINO LOI, VLADIMR KRYLOV AND JOSEF FULKA, LOI P (2006). The absence of DNA replication checkpoint in porcine zygotes. ZYGOTE, vol. 14, p. 33-37, ISSN: 0967-1994

 

 

 

FULKA J JR, MRAZEK M, FULKA H, LOI P (2005). Mammalian oocyte therapies. CLONING AND STEM CELLS, vol. 7, p. 183-188, ISSN: 1536-2302

 

 

LOI P (2004). Are there realistic opportunities to apply cloning and the other reproductive biotechnologies for safeguarding endangered species?. ANIMAL SCIENCE PAPERS AND REPORTS, vol. 22, p. 81-89, ISSN: 0860-4037

 

 

FULKA J JR, LOI P, FULKA H, PTAK G, NAGAI T. (2004). Nucleus transfer in mammals: noninvasive approaches for the preparation of cytoplasts. TRENDS IN BIOTECHNOLOGY, vol. 22, p. 279-283, ISSN: 0167-7799

 

 

 

Mattioli M, Gioia L, Turriani M, Capacchietti G, Loi P, Ptak G (2004). Oocyte maturation is required for correct sperm chromatin rearrangement. VETERINARY RESEARCH COMMUNICATIONS, vol. 28 (1), p. 205-208, ISSN: 0165-7380

 

 

LOI P., DELLA SALDA L, PTAK G (2004). Peri- and post-natal mortality of somatic cell clones in sheep. ANIMAL SCIENCE PAPERS AND REPORTS, vol. 22, suppl. 1, p. 59-70, ISSN: 0860-4037

 

 

BEAUJEAN N, TAYLOR JE, MCGARRY M, GARDNER JO, WILMUT I, LOI P, PTAK G, GALLI C, LAZZARI G, BIRD A, YOUNG LE, MEEHAN RR (2004). The effect of interspecific oocytes on demethylation of sperm DNA. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 20, p. 7636-7640, ISSN: 1091-6490

 

 

LOI P, FULKA J JR, PTAK G (2003). Amphibian and mammal somatic-cell cloning: different species, common results? (Cover page). TRENDS IN BIOTECHNOLOGY, vol. 21, p. 471-473, ISSN: 0167-7799


MATTIOLI M, BARBONI B, GIOIA L, LOI P (2003). Cold-induced calcium elevation triggers DNA fragmentation in immature pig oocytes. MOLECULAR REPRODUCTION AND DEVELOPMENT, vol. 65(3), p. 289-297, ISSN: 1040-452X, doi: 10.1002/mrd.10275

YOUNG LE, SCHNIEKE AE, MCCREATH KJ, WIECKOWSKI S, KONFORTOVA G, FERNANDES K, PTAK G, KIND AJ, WILMUT I, LOI P, FEIL R. (2003). Conservation of IGF2-H19 and IGF2R imprinting in sheep: effects of somatic cell nuclear transfer. MECHANISMS OF DEVELOPMENT, vol. 120, p. 1433-1442, ISSN: 0925-4773

PTAK GRAZYNA, TISCHENR MARIAN, BERNABÒ NICOLA, LOI P (2003). Donor-Dependent Developmental Competence of Oocytes from Lambs Subjected to Repeated Hormonal Stimulation. BIOLOGY OF REPRODUCTION, vol. 69 (1), p. 278-285, ISSN: 0006-3363

FULKA J, MOOR RM, LOI P, FULKA J (2003). Enucleolation of porcine oocytes. THERIOGENOLOGY, vol. 59, p. 1879-1885, ISSN: 0093-691X

FULKA J JR, LOI P, FULKA H, KREN R, DEAN W, MRAZEK M, REIK W (2003). Nucleus replacement in Mammalian oocytes. CLONING AND STEM CELLS, vol. 4, p. 181-187, ISSN: 1536-2302

LOI P, BARBONI B, PTAK G (2002). Cloning advances and challenges for conservation. TRENDS IN BIOTECHNOLOGY, vol. 20, p. 233, ISSN: 0167-7799, doi: 10.1016/S0167-7799(02)01955-8
PTAK G., CLINTON M., TISCHNER M., BARBONI B., MATTIOLI M, LOI P. (2002). Improving delivery and offspring viability of in vitro-produced and cloned sheep embryos. BIOLOGY OF REPRODUCTION, vol. 67(6), p. 1719-1725, ISSN: 0006-3363, doi: 10.1095/biolreprod66.3.796

PLOI P, CLINTON M., BARBONI B., FULKA J. JR., CAPPAI P., FEIL R., MOOR R.H.M., PTAK G. (2002). Nuclei of nonviable ovine somatic cells develop into lambs after nuclear transplantation.. BIOLOGY OF REPRODUCTION, vol. 67, p. 126-132, ISSN: 0006-3363, doi: 10.1095/biolreprod67.1.126

PTAK G., CLINTON M., BARBONI B., MUZZEDDU M., CAPPAI P., TISCHNER M., LOI P (2002). Preservation of the wild European mouflon: the first example of genetic management using a complete program of reproductive biotechnologies. BIOLOGY OF REPRODUCTION, vol. 66, p. 796-801, ISSN: 0006-3363, doi: 10.1095/biolreprod66.3.796

LOI P, PTAK G, BARBONI BARBARA, FULKA JOSEF JR, CAPPAI PIETRO, MICHAEL CLINTON (2001). Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells. NATURE BIOTECHNOLOGY, vol. 19, p. 962-964, ISSN: 1087-0156

FULKA J. JR, LOI P, LEDDA S, MOOR RHM, FULKA J (2001). Nucleus transfer in mammals: how the oocyte cytoplasm modifies the transferred nucleus. THERIOGENOLOGY, vol. 55, p. 1373-1380, ISSN: 0093-691X

J. FULKA JR, J. TESARIK, LOI P, R.M. MOOR (2000). Manipulating the Human embryo: Cell Cycle Checkpoint Controls. CLONING AND STEM CELLS, vol. 2, p. 1-7, ISSN: 1536-2302

FULKA J JR, TESARÍK J, LOI P, MOOR RM (2000). Manipulating the human embryo: cell cycle checkpoint controls. CLONING AND STEM CELLS, vol. 2, p. 1-7, ISSN: 1536-2302

PLOI P, FULKA J.JR., NAITANA S., CAPPAI P. (1999). Cloning by nuclear transfer: experiences in sheep. LIVESTOCK PRODUCTION SCIENCE, vol. 60, p. 281-294, ISSN: 0301-6226

PTAK G, LOI P., DATTENA M., TISCHNER M., CAPPAI P. (1999). Offspring from one-month-old lambs: studies on the developmental capability of prepubertal oocytes. BIOLOGY OF REPRODUCTION, vol. 61, p. 1568-1574, ISSN: 0006-3363

 

PTAK G., DATTENA M., LOI P, TISCHNER M., CAPPAI P (1999). Ovum pick-up in sheep: efficiency of in vitro embryo production, vitrification and offspring. THERIOGENOLOGY, vol. 52, p. 1105-1114, ISSN: 0093-691X

LOI P, PTAK G., DATTENA M., LEDDA S., NAITANA S., CAPPAI P (1998). 26.Embryo transfer and related technologies in sheep reproduction. REPRODUCTION NUTRITION DEVELOPMENT, vol. 38, p. 615-628, ISSN: 0926-5287

FULKA J. JR. FIRST N. L., LOI P, MOOR R. M (1998). Cloning by somatic cell nuclear transfer. BIOESSAYS, vol. 20, p. 847-851, ISSN: 0265-9247

LOI P, LEDDA S., FULKA J.JR., CAPPAI P., MOOR R.M. (1998). Development of parthenogenetic and cloned ovine embryos: effect of activation protocols. BIOLOGY OF REPRODUCTION, vol. 58, p. 1177-1187, ISSN: 0006-3363

FEIL R, KOSHLA S, CAPPAI P, LOI P (1998). Genomic imprinting in ruminants: allele-specific gene expression in parthenogenetic sheep. MAMMALIAN GENOME, vol. 9, p. 831-834, ISSN: 0938-8990

 

NAITANA S., LEDDA S., LEONI G., BOGLIOLO L., LOI P, CAPPAI P (1998). Membrane integrity and fertilizing potential of cryopreserved spermatozoa in European mouflon. ANIMAL REPRODUCTION SCIENCE, vol. 52, p. 105-112, ISSN: 0378-4320

 

 

LEDDA S, BOGLIOLO L, LEONI G, LOI P, CAPPAI P, NAITANA S (1998). Two culture systems showing a biphasic effect on ovine embryo development from the 1-2 cell stage to hatched blastocysts. REPRODUCTION NUTRITION DEVELOPMENT, vol. 35, p. 629-637, ISSN: 0926-5287


CAMPBELL K.H.S., LOI P, OTAEGUI P., WILMUT I (1997). Cell cycle co-ordination in embryo cloning by nuclear transfer. JOURNAL OF REPRODUCTION AND FERTILITY, vol. 1, p. 40-46, ISSN: 0022-4251

LOI P, BOYAZOGLU S., GALLUS M., BRANCA A., LEDDA S., NAITANA S., WILMUT I., CAPPAI P (1997). Embryo Cloning in Sheep: work in progress. THERIOGENOLOGY, vol. 48, p. 1-10, ISSN: 0093-691X
NAITANA S, LEDDA S, LOI P, LEONI G, BOGLIOLO L, DATTENA M, CAPPAI P (1997). Polyvinyl alcohol as a defined substitute for serum in vitrification and warming solutions to cryopreserve ovine embryos at different stages of development. ANIMAL REPRODUCTION SCIENCE, vol. 48, p. 247-256, ISSN: 0378-4320

CAMPBELL KH, LOI P, OTAEGUI PJ, WILMUT I (1996). Cell cycle co-ordination in embryo cloning by nuclear transfer. REVIEWS OF REPRODUCTION, vol. 1, p. 40-46, ISSN: 1359-6004

NAITANA S, LOI P, LEDDA S, CAPPAI P, DATTENA M, BOGLIOLO L, LEONI G (1996). Effect of biopsy and vitrification on in vitro survival of ovine embryos at different stages of development. THERIOGENOLOGY, vol. 46, p. 813-824, ISSN: 0093-691X

LEDDA S., LOI P, BOGLIOLO L., MOOR R.M. FULKA J. JR. (1996). The effect of 6- Dimethylaminopurine (6-DMAP) on DNA synthesis in activated mammalian oocytes. ZYGOTE, vol. 4, p. 7-9, ISSN: 0967-1994

CAMPBELL K.H.S., LOI P, CAPPAI P., WILMUT I (1994). Improved development to blastocyst of ovine nuclear transfer embryos reconstructed during the presumptive S-Phase of enucleated-activated oocytes. BIOLOGY OF REPRODUCTION, vol. 50, p. 1385-1393, ISSN: 0006-3363

Prodotti della ricerca

 

Funding ID

COORDINATOR, H2020, MSCA RISE 2016-2021 GA 734434. Setting an interdisciplinary/sectorial/international research network to explore dry storage as an alternative strategy for cells/germplasm biobanking.  1530000 €

COORDINATOR,H2020 MSCA IF 2017-2019. GA  749537, Drystore. Exploring dry storage as an alternative biobanking strategy inspired by Nature. 188000 €

PI H2020 Twinning Action 2016-2019. GA 698165. Epigenetic Risk Assessment of Assisted Reproductive Technologies. 295000 €

COORDINATOR, MIUR PRIN 2009: I 2009JE3CHM Induction and evaluation of multipotency in embryonic… of veterinary interest, 370,000 €

COORDINATOR,MIUR PRIN 2006 Project: 2006078015_002 Cell therapy: development of large animal models for bio-medical and pre-clinical studies, 147000 €

COORDINATOR,PMIUR PRIN 2002: cod. 2002074357 Isolation and characterization of adult and embryonic stem cells… differentiation plasticity 115,000 €

COORDINATOR,MIUR FIRB Project Negotiating Projects 2001: RBNE01HPMX Identification of mechanisms that regulate the expression of genes of … of domestic animals . 1250000 €

PI Project FP7-KBBE-2009-3: Next generation methods to preserve farm animal biodiversity by optimizing present and future breeding options. 135,000 €

PI European Science Foundation grant "Stellar" 2004-007 "Novel approaches to enhance…. research" acronym; EuroSTELLS, VI FP EU contract no. ERAS-CT-2003-980409; 154000 €

COORDINATOR, MIUR Interlink: II04C0H723 Study of the expression of single genes (DNMT1), and of the…. post implantation period. 45000 €

PI United States Department of Agriculture (USDA) "Proposal # 2007-01215

"Deregulation of Placenta vascular development in ART-derived sheep fetuses, $ 350,000

PI MIUR FIRB Establishing a Genome Resort for animal breeding and selection. GenHome cod. 0070120.  678,000 €

COORDINATOR,Tercas Foundation "Development of protocols for the freeze-drying of cell lines and embryos ", 100,000 €

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Ultimo aggiornamento: 10-06-2019
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