PERSONAL INFORMATION

Pasqualino Loi

ORCID: 0000-0003-4631-7663

 

WORK EXPERIENCE

01/01/1990–01/08/1992 Junior Scientist, embryologist Istituto Zootecnico & Caseario, Minister of Agriculture, Sassari, Italy, Sassari (Italy) Research activity in the field of farm animal reproduction

01/09/1992–01/10/1994 Post Doc, Roslin Institute, Edinburgh (United Kingdom)
Research activity in the field on nuclear transfer, in the Group of Ian Wilmut and Keith Campbell

01/09/1994–01/10/1994 EMBO Fellow University of Newcastle Upon Tyne, Newcastle (United Kingdom) Fellowship on Gene Expression Analysis in Embryos

01/11/1994–30/10/2000 Senior Scientist, Istituto Zootecnico & Caseario, Minister of Agriculture, Sassari (Italy)

11/11/2000–30/12/2019 Associate Professor in Animal Physiology, Faculty of Veterinary Medicine University of Teramo, MIUR, Teramo (Italy) Research, Teaching and Institutional Activities

01/01/2019–Present Full Professor in Animal Physiology Research University of Teramo, MIUR, Teramo (Italy),
Teaching and Institutional Activities, Faculty of Veterinary Medicine

EDUCATION AND TRAINING

01/11/1980–06/07/1986. Bachelor in Veterinary Medicine, University of Sassari, Sassari (Italy) EQF 7

01/11/1987–05/06/1999. PhD in Developmental Biology, University of Sassari, Sassari (Italy) EQF 8

 

PERSONAL SKILLS

 

Mother tongue(s) Italian

Foreign language(s)

UNDERSTANDING

SPEAKING

WRITING

ENGLISH

C2

C2

C2

FRENCH

A2

A2

A2

POLISH

A2

A2

A2

 

Communication skills

Synthesis. Excellent communication skills gained in 20 years of continuous teaching to undergraduate, PhD students; tutoring post docs, delivering talks to international meetings, continuous engagement in outreach activities, interviews by leading press (details below).

 

I have 22 years teaching experience for undergraduate students (Course of Veterinary Medicine, 5 years; Biotechnology, Bachelor; Reproductive biotechnologies, Master), of the following courses:

• Veterinary Physiology, 8 CFU, 88 hours
• Technologies for cell culture and stem cells, 6 CFU, 60 hours
• Experimental Embryology, 7 CFU, 70 hours

2016-2017 External Teacher for the Giessen Graduate School for Life Sciences, Giessen, Germany

2017-2018 External Teacher of the PhD program of the Tartu University, Estonia,

2013 - to present External Teacher Biotechnology Master, University of Florence, Italy

2017- to present External Teacher Reproduction Biotechnology Master, University of Bologna, Italy

2017 External Examiner, PhD defences, University of Nottingham, United Kingdom

2011 External Examiner, PhD defences, Polish Academy of Sciences, Warsaw, Poland

2017 External Examiner, PhD defences, University of Bologna, Italy

2020 External Examiner, PhD defences, University of Turin, Italy

 

Invitation as a Speaker in International Conferences (National ones no listed!)

Invited speaker, ICAR, 2026, 24-26 June, in Obihiro, Japan. Workshop 15: Preservation of the genetic resources of farm animals by cryopreservation of oocytes/embryos and drying of sperm and somatic cell' at IInter ntional Congreess of Animal Reproduction, ICAR, 2026.

 

Invited Speaker, European Society for Human Reproduction, ESHRE Workshop, Essentials of Vitrification,  Futuristic cell preservation: is dehydration and rehydration of oocytes the next chapter? Zurich, Switzerland, 23-25 October 2025

 

Invited speaker, ART Fertility  8^th conference on Cutting-Edge Advances in Human Assisted Reproduction Technologies ART, 2025, 11^th April, St. Regis Saadiyat , Abu Dhabi

 

Invited Speaker  to the 26^th International Congress on Reproductive Biomedicine (3-5 September 2025)  in the following session: Advances in Reproductive Biotechnology

Friday 05 September 2025, 11:00-13:00, Allame Tabatabaee Hall, Iran

 

Invited Speaker, Program T u r k i s h  a n d  I t a l i a n  J o i n t  I n t e r n a t i o n a l  C o n g r e s s  o f  A n i m a l  R e p r o d u c t i o n,   “Innovations in Non Cryogenic, Dry Storage of Spermatozoa for Farm Animal Assisted Reproduction, Antalya, Turkey, 10^th October 2024

Invited Speaker International Embryo Technology Society 47th Annual Conference, Lima, Peru, January 2021

Invited Speaker Ovarian Club, XIV Symposium, Paris, November 7-9 2020, France. 

Invited Speaker Ovarian Club, XIV Symposium, Paris, November 7-9 2019, France. 

Invited Speaker Ovarian Club, XIV Symposium, Paris, November 7-9 2018, France. 

Invited Speaker World Congress of Reproduction Biology 2017 (WCRB2017), Okinawa, Japan, 27nd-29th September 2017.

Invited Speaker "Laudazio” Prof. Cesare Galli: recipient of the AETE pioneer award 2017". 33 Meeting of the Association European for the Embryo Transfer (AETE). Bath, 8-9 September 2017, UK

 

Invited Speaker  University of Giessen, Germany :Winter Semester 2016/2017 GGL - Section 6 (Reproduction in Men and Animals) -Alternative dry storage bio-banking of cells, gametes and embryos 25/01/2017

 

Invited Speaker VI International Symposium of Animal Biology of Reproduction. Campos so Jordao Convenction Center :Session II: Cell Reprogramming 14:00 to 14:45 Dr. Pasqualino Loi (Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy) Lecture 6: Alternative strategies for nuclear reprogramming in Somatic Cell Nuclear transfer (SCNT). 14:45 to 15:30  06/11/2016    

 

Invited Speaker North Sumatra White Ryno Working Group: Conservation by Cellular Technologies, Vienna, 3-5 December 2015 Pasqualino Loi: Limited availability of foster mothers a Major problem in ART for species salvation           03/12/2015     05/12/2015    

                       

Invited Speaker Cost Action Epiconcept, Meeting Conference 2015, Epigenetics and Periconception Environment 06-07 October 2015, Hersonissos, Crete, GreeceWorking Group 4: Public, Periconception and Epigenome 14:00 – 14:40 (40:00) Pasqualino Loi, University of Teramo, Italy Progress in cloning. How it should be: science from laboratory to people     06/10/2015    

           

Invited Speaker Cost Action Epiconcept, Meeting Conference 2015, Epigenetics and Periconception Environment, Hersonissos, Crete, Greece, Working Group 4: Public, Periconception and Epigenome Loi, University of Teramo, Italy Cloning the mammoth. The other way around: science from people to the laboratory. 06-07 October 2015.

 

Invited Speaker 41 International Embryo Transfer Society Meeting, Versailles, France, Concurrent Session:16:30–18:00 CANDES Forum (Foyer Condé, Vauban, and Pascal) Session chair: Gabriela Mastromonaco, Toronto Zoo Presentations of selected topics from the book titled "Reproductive Sciences in Animal Conservation: Progress and Prospects" will be given by the contributing authors: Pasqualino Loi -Could cloning ever be used to resurrect extinct species? A critical appraisal10/01/2015

 

Invited Speaker ISAFG (International Symposium of Functional Genomics Piacenza, 27th - 29th of July, 2015, Piacenza, Italy: Restoring a correct epigenome in ART derived pre- and post implantation embryos, including cloned ones  27/07/2015     29/07/2015    

 

Invited Speaker 3rd International Congress on Controversies in Cryopreservation of Stem Cells, Reproductive Cells, Tissue, Organs & Cryo Surgery (CRYO)21 - 23 March, 2013 Berlin, Germany: Pasqualino Loi: Late Embryogenesis Abundant (LEA) and desiccation tolerance in Sheep Oocytes

 

Invited Speaker TEDXLakeComo: Il Futuro Non Accade per Caso. Pasqualino Loi: La Nuova Arca di Noe https://www.ted.com/tedx/events/4631         10/11/2012    

 

Invited Speaker Leibniz Institute for Zoo and Wildlife Research (IZW) Alfred-Kowalke-Str. Berlin, Germany. Novembre 23, 2012. Pasqualino Loi: Cloning using Lyophilized Cells23/11/2012

 

Invited Speaker Seminar, Makerere University, Uganda, Pasqualino Loi: Biobanking   23/01/2011     27/01/2011    

                       

Invited Speaker The 2nd International Congress on Controversies in Cryopreservation of Stem Cells, Reproductive Cells, Tissue & Organs (CRYO) Palacio de Congresos, Valencia, Spain, April 7-9, 2011. Pasqualino Loi: Freeze Drying Oocytes        07/04/2011     09/04/2011

                                  

Invited Speaker The Oocyte: from Basic Research to Clinical Practice Princesa Sofia Hotel, Barcelona, Spain, November 3-6, 2011 dried oocytes and "micro-cytoplast" P. Loi, Italy http://comtecmed.com/oc/2011/timetable.aspx           03/11/2011     06/11/2011

                                  

Invited Speaker The Oocyte: from Basic Research to Clinical Practice Princesa Sofia Hotel, Barcelona, Spain, November 3-6, 2011. Pasqualino Loi: Nuclear transfer  03/11/2011

 

Invited Speaker Centre for Cellular and Molecular Biology, Hyderabad- First Lacones Foundation Lecture. P. Loi: Cloning For Biodiversity Preservation. 1st February 2008, Hyderabad, India. 01/02/2008

                                  

Invited Speaker 16th International Congress on Animal Reproduction (ICAR, the most important congress on animal reproduction) key lecture: “Use of Freeze Dried Cells for nuclear transfer. 13-17 July 2008. Budapest, Hungary.     13/07/2008     17/07/2008    

Invited Speaker Eurobiotech 2008 Central European Congress of Life Sciences. Session 2- Mammalian Cloning. 17-19 October 2008, Krakow, Poland 17/10/2008     19/10/2008

Invited Speaker 2nd Annual Lyophilisation Meeting. “Boosting stability, delivery and longevity for pharmaceuticals and biotechnology”. 15th - 17th September 2009 , BSG Conference Centre, London, UK.

Invited Speaker National Institute of Livestock and Grassland Science Seminars: “Overview of Nuclear Reprogramming Strategies and some Suggestions”, 3rd September 2007, Riken Biocenter, Tsukuba, Japan 03/09/2007.

 

Invited Speaker Institut fur Tierzucht Seminars: Pasqualino Loi: “Asymmetry in Nuclear Reprogramming in Somatic Cells Nuclear Transfer? 19 April 2007, Mariensee, Neustadt, Germany. 19/04/2007.

 

Invited Speaker Polish Academy of Sciences Meeting (organized in the framework of the EU founded Centre of Excellence Animbiogen), P. Loi : “Somatic Cloning as a tool in Animal Biotechnology”. Wierzba, 18-21 January 2004.  18/01/2004     21/01/2004.

                       

Invited Speaker European Society of Human Reproduction (ESHRE), Workshop on: “Mammalian Oogenesis and Folliculogenesis: in vivo and in vitro approaches”. Frascati, 10-12 April 2003, Italy.

 

Invited Speaker Serono Symposia International Meeting: Genesis and Fate of the Preimplantation Embryo: Intrinsic and Extrinsic Influences”. Sorrento, 29 September, 1st October 2002, Italy.

                                  

Invited Speaker Roslin Institute Seminars. P Loi: “Can We Regulate Nuclear Reprogramming?” Roslin Institute, 8 March 2002, Conference Hall, Midlothian, Edinburgh UK. 08/03/2002

 

Major Collaborations

Dr Saadi Khochbin, INSERM, U823, Institut Albert Bonniot, Université Grenoble Alpes, France

Dr. Atsuo Ogura, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan

Prof. Thomas Hildebrandt, Wildlife reproduction, The Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany

Dr. Michael Clinton, Roslin Institute, Edinburgh, UK

Prof. Jacek Modlinski, Institute of Animal Genetics, Polish Academy of Sciences, Warsaw, Poland.

Prof. Katsuhiko Hayashi, Department of Developmental Stem Cell, Faculty of Medical Sciences, Kyushu University, Japan.

Prof. Paolo Ajmone Marsan, Università Cattolica del Sacro Cuore, Piacenza, Italy

Dr. Robert Feil, Genomic Imprinting Group, CNR, Grenoble, France.

Dr. John Williams, University of Adelaide, Australia

Dr. Alessandra Stella, Consiglio Nazionale della Ricerca, Italy.

Prof. Lawrence Reynolds, North Dakota State University, USA

Dr. Josef Fulka, Institute of Animal Science, 104 00, Prague 10, Czech Republic.

Prof. Kazutsugu Matsukawa, Kochi University, Japan.

Dr. Takahiro Kikawada, Institute for Insect Biology, NIAS, Tsukuba, Ibaraki, Japan

Prof. Cesare Galli, Avantea srl (SME), Cremona, Italy

 

Prizes/ Awards/ Academy memberships: 

1994 EMBL Fellow, University of Newcastle upon Tyne, UK. 

2016 Best Scientific Contribution: International Symposium on the Future of Nuclear Transfer and Nuclear Reprogramming, Tsukuba, Riken Institute, Japan. 

2017 Japanese Society for the Promotion of Science (JSPS) Fellow, Riken Institute, Tsukuba, Japan. 

Latest Dissemination as influential Scientist in my field:

https://www.nature.com/news/endangered-species-sex-and-the-single-rhinoceros-1.10731

https://www.scientificamerican.com/article/scientists-successfully-

https://www.scientificamerican.com/article/spry-dolly-siblings-could-make-clone-skeptics-sheepish

https://www.scientificamerican.com/article/cloning-endangered-animals/

https://www.scientificamerican.com/article/rhinoceros-assisted-reproduction-rescue-sepcies 

http://www.nytimes.com/2016/07/27/science/dolly-the-sheep-clones.html?_r=0;

https://doi.org/10.1038/nature.2012.10228

 

Organisational / managerial skills

Institutional Responsibilities

2000- Group Leader of 5-12 people (Experimental Embryology Group), including permanent staff, PhD and Post Docs.

2000- to present - Scientific Coordination of large national and international research projects (detail below)

2019 – to present. Leader of the Research Steering Committee of the Faculty of Veterinary Medicine

2019 – to present. Member of the Senate of University of Teramo

2018 - to present. Responsible for undergraduate students with learning problem for the Veterinary Faculty

2010 - to present. Faculty Committee member for the European Association for Education Veterinary medicine (EAEVE)

2000 - to present. Member of the Council of the Faculty of Veterinary Medicine, University of Teramo

 

2001 - to present. Member of the Committee of the PhD programme in Veterinary sciences

 

Commissions of Trust

2009-2011 Scientific Advisory Board, of EU ANIMBIOGEN, VII FP EU - institute of Animal Genetics, Polish Academy of Sciences, Warsaw, Poland 2009-2012,

2007 - present– Review Board member for the European Science Foundation (EFS).

2010-2013 Managing Committee Deputy of the EU COST - “Gemini”, VII FP EU.

2014-2017 Managing Committee Deputy of the EU COST - “Epiconcept”, VII FP EU.

 

Prizes/ Awards/ Academy memberships;

Japanese Society for the Promotion of Science (JSPS) Fellow, Riken Institute, Tsukuba, Japan

Best Scientific Contribution: 2016.03.10 International Symposium on the Future of Nuclear Transfer and Nuclear Reprogramming, Tsukuba, Riken Institute, Japan.

 

Major contributions to the early careers of excellent researchers; - Several of my former PhDs/Post Docs are permanently employed in prestigious research Institutions: Prof. G. Ptak, ERC grantee (Starting Grant) under my guidance, University Jagellonian Krakow, Poland; Dr. Lopes F., University of Edinburgh, UK; Dr. Fidanza A., Medical Research Council, Edinburgh, UK; Dr. Sampino S. Polish Academy of Sciences, Poland; Dr. Iuso D., University of Grenoble, France; Dr. Turco-Yaioy M:, University of Cambridge, UK; Prof. Matsukawa K., University of Kochi, Japan

 

Organization of International Meetings

Scientific Committee International Congress on Animal Reproduction (ICAR), Bologna June 27-2 July 2020 (postponed to 2022, same dates)

 

Scientific Committee Workshop "Micromanipulation (of oocyte/embryo)" Managed jointly with Jan Detterer (Germany). 33 Meeting AETE, Bath, 8-9 September 2017; UK         08/09/2017                

 

Scientific Committee International Conference “Epigenetic Penetrance of Reproductive Technologies”, University of Teramo, 9-10 June 2016, project EU Horizon2020 "EraofArt", Twinn action 09/06/2016, 10/06/2016.

 

Scientific Committee Workshop “Pre-implantation embryos development in large animals: oocytes maturation, fertilization, embryo culture and cryopreservation”, University of Teramo, 11-22 April 2016 EU Horizon2020 "EraofArt", Twinn action                      

                                  

Scientific Committee European Science Foundation Programme “EuroSTELLS”: Workshop “Challenges in Stem Cell Differentiation and Transplantation” http://www.esf.org , 30 September – 3 October 2007, Milan, Italy.        30/09/2007     03/10/2007    

 

Scientific Committee European Science Foundation Programme “EuroSTELLS”: Workshop “Stem Cell Niches” http://www.esf.org/media-centre/press, 10-12 Gennaio 2008, Barcellona, Spain10/01/2008 12/01/2008

 

Editorial Board member

Editorial Board Member Journal “Animal Science Papers and Reports”. ISSN 0860-4037

Editorial Board Member Journal "Cellular Reprogramming". ISSN: 2152-4971

Editorial Board Member of “Journal of Reproduction and Development”, ISSN: 1348-4400

 

Reviewer, ad hoc

Nature, Science, Biology of Reproduction, Theriogenology, Gene, Trends in Biotechnology, Cellular Reprogramming, Theriogenology, Reproduction, Fertility and Development, Gene, Genomics, Reproduction in Domestic Animals, Animal Reproduction Science, Journal of Assisted Reproduction Genetics, PLoS ONE, Frontiers series.

 

Evaluator for funding agencies

2007 –Referee for the European Research Council –remote evaluation - (ERC), VII FP EU

2010-2014 Referee for the Marie Curie Fellowship Programme, VII FP EU

2011-2019 Referee for the Polish Academy of Sciences

2013 Referee for the Royal Society, UK

2013 Referee for the USA Israel Bi-National Scientific Programme

2015-2019 Referee for the Cyprus Academy of Sciences

2017-to present Referee for the European Science Foundation

2018 Referee for the Lithuanian National Science Foundation

2018 Referee for the Medical Research Council, UK

2019 Referee - expert pool -for the Fonds Wetenschappelijk Onderzoek – Vlaanderen (FWO), Belgium

2018-to present Referee for the Dutch Science Foundation

2018-to present Referee for the Czech Academy of Sciences

2010-to present Referee for the Italian Minister of Research and University (MIUR)

2021-to present Referee for the Natural Sciences and Engineering Research Council of Canada

2022-to present Referee for the  French National Research Agency (ANR)

2022-to present Referee for the Spanish National Research Agency.

2025-to present, Referee for the Slovak National Research Agency.

 

Membership of Scientific Societies

Italian Society of Veterinary Sciences,

Italian Association of Veterinary Physiologists

International Embryo Transfer Association (IETS)

Society for Reproduction and Fertility (SRF)

 

Job-related skills

Short Biographical Sketch

I have started to work on embryos 30 years ago as a self-made man in a small Veterinary Faculty in Sardinia, Italy. I have dedicated a great deal of efforts on embryo multiplication by splitting, blastomere separation and embryonic cells nuclear transfer during my PhD. Then I joined Keith Campbell and Ian Wilmut at the Roslin Institute, Edinburgh, Scotland, UK, to work on a sheep nuclear transfer project aiming at cell cycle coordination between enucleated oocytes and donor embryonic nuclei. The publications jointly delivered (Campbell, KHS Loi et al., Reviews of Reproduction, 1996; Campbell KHS, Loi P., Biol Reprod. 1994) are milestones in nuclear transfer research and prepared the ground for the production of the first sheep cloned from embryonic (Campbell et al., Nature 1996;380: 64-6) and next adult cell lines (Wilmut et al., Nature 1997, 385: 810-3). After my work at Roslin, I continued to work on Somatic Cell Nuclear Transfer (SCNT) producing the first ever wild animal by cloning (Loi et al. Nature Biotechnology, 2001). SCNT still remains my primary research interest where I have given significant contributions. I have in fact demonstrated for the first time that non-living cells can generate offspring upon SCNT (Loi et al., Biol Reprod. 2002), a breakthrough that has prepared the ground for cloning lyophilized cells (Loi et al., PloS One 2018), till the most recent attempts to clone the mammoth (Yamagata et al., 2019, Sci Rep. 2019; 9:4050). My commitment to elucidate the mechanisms for nuclear reprogramming after SCNT continues, and the latest outcomes of my research group represent a valid option to improve nuclear reprogramming by conferring somatic cells the nuclear structure of a spermatid cells, by the heterologous expression of protamine1 (Iuso et al., Cell Reports 2015; Czernik et al., Nature Protocols 2016; Palazzese et al., Plos One, 2018).

Metrics - My H Index on Google Scholar is 39, with 5113 citations. Please, note the Veterinary Sciences publications have a lower IF comparing to other branch of science; therefore, my H Index for veterinary research is high.

 

Granted patents:

 

Pasqualino Loi et al., Improved method for reconstructing a non-human animal embryo EP 3134532 A1 (WO2015162170A1), PCT/EP2015/058701

 

Pasqualino Loi & Pietro Cappai. Process for reconstructing a non-human mammalian embryo by nuclear transfer using a heat treated donor nucleus and preparing a non-human mammal US 7064245 B1;PCT/IT1999/000160

 

 

 

Research Support

Ongoing Research Support

 

Project Title

 

Funding source

 

Amount

(Euros)

 

Period

 

Role

 

Aims

Drynet

Horizon 2020, No 734434 MSCA Action “RISE”,

1250000

2017-2021

Coordinator

Exploring dry storage of cells, gametes and embryos

Completed Research Support

Coordinator, EU, Horizon2020, MSCA SE, WhyNotDry, 670000 euros, 2023-2027

Coordinator, EU Horizon2020, MSCA-IF-2’16, “Drystore” GA 749537 188000 euro    2017-2019, Development of drying protocols for gametes and cells

PI EU Horizon 2020, Twinning action,  “EraofArt”, GA 698165   295000 euro   2016-2019      Beneficiary Partner    Contemplates major embryo Biotechnologies

PI European Science Foundation grant "Stellar" EUROCORES Programme EuroSTELLS, supported by FP VI EU contract no. ERAS-CT-2003-980409; 154000 euro 2007-2011

PI United States Department of Agriculture (USDA) "Proposal#2007-01215 "" 350.000 $, 01/01/2005/01/2008

PI FP7 EU KBBE-2009-3: Next generation methods to preserve farm animal biodiversity by optimizing present and future breeding options. 130000 euro.

 

Plus I have coordinated other National Grants (projects  Prin [n° 4, excellent research) and Firb [n° 2, basic research] from the Italian Minister for University and Education - MIUR), N° 6, with a total budget of about 5.000.000 euros, and grant supported by private (Bank Foundations N° 2, 150000 euro total budget)

 

 

Digital skills

Self-assessment grid

 

Information processing

communication

Content creation

Safety

Problem solving

Proficient user

Independent user

Basic user

Basic user

Basic user

 

Teramo, 27.08.2025

Yours Faithfully

 

 

 

 

 

PERSONAL INFORMATION

Pasqualino Loi

ORCID: 0000-0003-4631-7663

 

WORK EXPERIENCE

01/01/1990–01/08/1992 Junior Scientist, embryologist Istituto Zootecnico & Caseario, Minister of Agriculture, Sassari, Italy, Sassari (Italy) Research activity in the field of farm animal reproduction

01/09/1992–01/10/1994 Post Doc, Roslin Institute, Edinburgh (United Kingdom)
Research activity in the field on nuclear transfer, in the Group of Ian Wilmut and Keith Campbell

01/09/1994–01/10/1994 EMBO Fellow University of Newcastle Upon Tyne, Newcastle (United Kingdom) Fellowship on Gene Expression Analysis in Embryos

01/11/1994–30/10/2000 Senior Scientist, Istituto Zootecnico & Caseario, Minister of Agriculture, Sassari (Italy)

11/11/2000–30/12/2019 Associate Professor in Animal Physiology, Faculty of Veterinary Medicine University of Teramo, MIUR, Teramo (Italy) Research, Teaching and Institutional Activities

01/01/2019–Present Full Professor in Animal Physiology Research University of Teramo, MIUR, Teramo (Italy),
Teaching and Institutional Activities, Faculty of Veterinary Medicine

EDUCATION AND TRAINING

01/11/1980–06/07/1986. Bachelor in Veterinary Medicine, University of Sassari, Sassari (Italy) EQF 7

01/11/1987–05/06/1999. PhD in Developmental Biology, University of Sassari, Sassari (Italy) EQF 8

 

PERSONAL SKILLS

 

Mother tongue(s) Italian

Foreign language(s)

UNDERSTANDING

SPEAKING

WRITING

ENGLISH

C2

C2

C2

FRENCH

A2

A2

A2

POLISH

A2

A2

A2

 

Communication skills

Synthesis. Excellent communication skills gained in 20 years of continuous teaching to undergraduate, PhD students; tutoring post docs, delivering talks to international meetings, continuous engagement in outreach activities, interviews by leading press (details below).

 

I have 22 years teaching experience for undergraduate students (Course of Veterinary Medicine, 5 years; Biotechnology, Bachelor; Reproductive biotechnologies, Master), of the following courses:

• Veterinary Physiology, 8 CFU, 88 hours
• Technologies for cell culture and stem cells, 6 CFU, 60 hours
• Experimental Embryology, 7 CFU, 70 hours

2016-2017 External Teacher for the Giessen Graduate School for Life Sciences, Giessen, Germany

2017-2018 External Teacher of the PhD program of the Tartu University, Estonia,

2013 - to present External Teacher Biotechnology Master, University of Florence, Italy

2017- to present External Teacher Reproduction Biotechnology Master, University of Bologna, Italy

2017 External Examiner, PhD defences, University of Nottingham, United Kingdom

2011 External Examiner, PhD defences, Polish Academy of Sciences, Warsaw, Poland

2017 External Examiner, PhD defences, University of Bologna, Italy

2020 External Examiner, PhD defences, University of Turin, Italy

 

Invitation as a Speaker in International Conferences (National ones no listed!)

Invited speaker, ICAR, 2026, 24-26 June, in Obihiro, Japan. Workshop 15: Preservation of the genetic resources of farm animals by cryopreservation of oocytes/embryos and drying of sperm and somatic cell' at IInter ntional Congreess of Animal Reproduction, ICAR, 2026.

 

Invited Speaker, European Society for Human Reproduction, ESHRE Workshop, Essentials of Vitrification,  Futuristic cell preservation: is dehydration and rehydration of oocytes the next chapter? Zurich, Switzerland, 23-25 October 2025

 

Invited speaker, ART Fertility  8^th conference on Cutting-Edge Advances in Human Assisted Reproduction Technologies ART, 2025, 11^th April, St. Regis Saadiyat , Abu Dhabi

 

Invited Speaker  to the 26^th International Congress on Reproductive Biomedicine (3-5 September 2025)  in the following session: Advances in Reproductive Biotechnology

Friday 05 September 2025, 11:00-13:00, Allame Tabatabaee Hall, Iran

 

Invited Speaker, Program T u r k i s h  a n d  I t a l i a n  J o i n t  I n t e r n a t i o n a l  C o n g r e s s  o f  A n i m a l  R e p r o d u c t i o n,   “Innovations in Non Cryogenic, Dry Storage of Spermatozoa for Farm Animal Assisted Reproduction, Antalya, Turkey, 10^th October 2024

Invited Speaker International Embryo Technology Society 47th Annual Conference, Lima, Peru, January 2021

Invited Speaker Ovarian Club, XIV Symposium, Paris, November 7-9 2020, France. 

Invited Speaker Ovarian Club, XIV Symposium, Paris, November 7-9 2019, France. 

Invited Speaker Ovarian Club, XIV Symposium, Paris, November 7-9 2018, France. 

Invited Speaker World Congress of Reproduction Biology 2017 (WCRB2017), Okinawa, Japan, 27nd-29th September 2017.

Invited Speaker "Laudazio” Prof. Cesare Galli: recipient of the AETE pioneer award 2017". 33 Meeting of the Association European for the Embryo Transfer (AETE). Bath, 8-9 September 2017, UK

 

Invited Speaker  University of Giessen, Germany :Winter Semester 2016/2017 GGL - Section 6 (Reproduction in Men and Animals) -Alternative dry storage bio-banking of cells, gametes and embryos 25/01/2017

 

Invited Speaker VI International Symposium of Animal Biology of Reproduction. Campos so Jordao Convenction Center :Session II: Cell Reprogramming 14:00 to 14:45 Dr. Pasqualino Loi (Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy) Lecture 6: Alternative strategies for nuclear reprogramming in Somatic Cell Nuclear transfer (SCNT). 14:45 to 15:30  06/11/2016    

 

Invited Speaker North Sumatra White Ryno Working Group: Conservation by Cellular Technologies, Vienna, 3-5 December 2015 Pasqualino Loi: Limited availability of foster mothers a Major problem in ART for species salvation           03/12/2015     05/12/2015    

                       

Invited Speaker Cost Action Epiconcept, Meeting Conference 2015, Epigenetics and Periconception Environment 06-07 October 2015, Hersonissos, Crete, GreeceWorking Group 4: Public, Periconception and Epigenome 14:00 – 14:40 (40:00) Pasqualino Loi, University of Teramo, Italy Progress in cloning. How it should be: science from laboratory to people     06/10/2015    

           

Invited Speaker Cost Action Epiconcept, Meeting Conference 2015, Epigenetics and Periconception Environment, Hersonissos, Crete, Greece, Working Group 4: Public, Periconception and Epigenome Loi, University of Teramo, Italy Cloning the mammoth. The other way around: science from people to the laboratory. 06-07 October 2015.

 

Invited Speaker 41 International Embryo Transfer Society Meeting, Versailles, France, Concurrent Session:16:30–18:00 CANDES Forum (Foyer Condé, Vauban, and Pascal) Session chair: Gabriela Mastromonaco, Toronto Zoo Presentations of selected topics from the book titled "Reproductive Sciences in Animal Conservation: Progress and Prospects" will be given by the contributing authors: Pasqualino Loi -Could cloning ever be used to resurrect extinct species? A critical appraisal10/01/2015

 

Invited Speaker ISAFG (International Symposium of Functional Genomics Piacenza, 27th - 29th of July, 2015, Piacenza, Italy: Restoring a correct epigenome in ART derived pre- and post implantation embryos, including cloned ones  27/07/2015     29/07/2015    

 

Invited Speaker 3rd International Congress on Controversies in Cryopreservation of Stem Cells, Reproductive Cells, Tissue, Organs & Cryo Surgery (CRYO)21 - 23 March, 2013 Berlin, Germany: Pasqualino Loi: Late Embryogenesis Abundant (LEA) and desiccation tolerance in Sheep Oocytes

 

Invited Speaker TEDXLakeComo: Il Futuro Non Accade per Caso. Pasqualino Loi: La Nuova Arca di Noe https://www.ted.com/tedx/events/4631         10/11/2012    

 

Invited Speaker Leibniz Institute for Zoo and Wildlife Research (IZW) Alfred-Kowalke-Str. Berlin, Germany. Novembre 23, 2012. Pasqualino Loi: Cloning using Lyophilized Cells23/11/2012

 

Invited Speaker Seminar, Makerere University, Uganda, Pasqualino Loi: Biobanking   23/01/2011     27/01/2011    

                       

Invited Speaker The 2nd International Congress on Controversies in Cryopreservation of Stem Cells, Reproductive Cells, Tissue & Organs (CRYO) Palacio de Congresos, Valencia, Spain, April 7-9, 2011. Pasqualino Loi: Freeze Drying Oocytes        07/04/2011     09/04/2011

                                  

Invited Speaker The Oocyte: from Basic Research to Clinical Practice Princesa Sofia Hotel, Barcelona, Spain, November 3-6, 2011 dried oocytes and "micro-cytoplast" P. Loi, Italy http://comtecmed.com/oc/2011/timetable.aspx           03/11/2011     06/11/2011

                                  

Invited Speaker The Oocyte: from Basic Research to Clinical Practice Princesa Sofia Hotel, Barcelona, Spain, November 3-6, 2011. Pasqualino Loi: Nuclear transfer  03/11/2011

 

Invited Speaker Centre for Cellular and Molecular Biology, Hyderabad- First Lacones Foundation Lecture. P. Loi: Cloning For Biodiversity Preservation. 1st February 2008, Hyderabad, India. 01/02/2008

                                  

Invited Speaker 16th International Congress on Animal Reproduction (ICAR, the most important congress on animal reproduction) key lecture: “Use of Freeze Dried Cells for nuclear transfer. 13-17 July 2008. Budapest, Hungary.     13/07/2008     17/07/2008    

Invited Speaker Eurobiotech 2008 Central European Congress of Life Sciences. Session 2- Mammalian Cloning. 17-19 October 2008, Krakow, Poland 17/10/2008     19/10/2008

Invited Speaker 2nd Annual Lyophilisation Meeting. “Boosting stability, delivery and longevity for pharmaceuticals and biotechnology”. 15th - 17th September 2009 , BSG Conference Centre, London, UK.

Invited Speaker National Institute of Livestock and Grassland Science Seminars: “Overview of Nuclear Reprogramming Strategies and some Suggestions”, 3rd September 2007, Riken Biocenter, Tsukuba, Japan 03/09/2007.

 

Invited Speaker Institut fur Tierzucht Seminars: Pasqualino Loi: “Asymmetry in Nuclear Reprogramming in Somatic Cells Nuclear Transfer? 19 April 2007, Mariensee, Neustadt, Germany. 19/04/2007.

 

Invited Speaker Polish Academy of Sciences Meeting (organized in the framework of the EU founded Centre of Excellence Animbiogen), P. Loi : “Somatic Cloning as a tool in Animal Biotechnology”. Wierzba, 18-21 January 2004.  18/01/2004     21/01/2004.

                       

Invited Speaker European Society of Human Reproduction (ESHRE), Workshop on: “Mammalian Oogenesis and Folliculogenesis: in vivo and in vitro approaches”. Frascati, 10-12 April 2003, Italy.

 

Invited Speaker Serono Symposia International Meeting: Genesis and Fate of the Preimplantation Embryo: Intrinsic and Extrinsic Influences”. Sorrento, 29 September, 1st October 2002, Italy.

                                  

Invited Speaker Roslin Institute Seminars. P Loi: “Can We Regulate Nuclear Reprogramming?” Roslin Institute, 8 March 2002, Conference Hall, Midlothian, Edinburgh UK. 08/03/2002

 

Major Collaborations

Dr Saadi Khochbin, INSERM, U823, Institut Albert Bonniot, Université Grenoble Alpes, France

Dr. Atsuo Ogura, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan

Prof. Thomas Hildebrandt, Wildlife reproduction, The Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany

Dr. Michael Clinton, Roslin Institute, Edinburgh, UK

Prof. Jacek Modlinski, Institute of Animal Genetics, Polish Academy of Sciences, Warsaw, Poland.

Prof. Katsuhiko Hayashi, Department of Developmental Stem Cell, Faculty of Medical Sciences, Kyushu University, Japan.

Prof. Paolo Ajmone Marsan, Università Cattolica del Sacro Cuore, Piacenza, Italy

Dr. Robert Feil, Genomic Imprinting Group, CNR, Grenoble, France.

Dr. John Williams, University of Adelaide, Australia

Dr. Alessandra Stella, Consiglio Nazionale della Ricerca, Italy.

Prof. Lawrence Reynolds, North Dakota State University, USA

Dr. Josef Fulka, Institute of Animal Science, 104 00, Prague 10, Czech Republic.

Prof. Kazutsugu Matsukawa, Kochi University, Japan.

Dr. Takahiro Kikawada, Institute for Insect Biology, NIAS, Tsukuba, Ibaraki, Japan

Prof. Cesare Galli, Avantea srl (SME), Cremona, Italy

 

Prizes/ Awards/ Academy memberships: 

1994 EMBL Fellow, University of Newcastle upon Tyne, UK. 

2016 Best Scientific Contribution: International Symposium on the Future of Nuclear Transfer and Nuclear Reprogramming, Tsukuba, Riken Institute, Japan. 

2017 Japanese Society for the Promotion of Science (JSPS) Fellow, Riken Institute, Tsukuba, Japan. 

Latest Dissemination as influential Scientist in my field:

https://www.nature.com/news/endangered-species-sex-and-the-single-rhinoceros-1.10731

https://www.scientificamerican.com/article/scientists-successfully-

https://www.scientificamerican.com/article/spry-dolly-siblings-could-make-clone-skeptics-sheepish

https://www.scientificamerican.com/article/cloning-endangered-animals/

https://www.scientificamerican.com/article/rhinoceros-assisted-reproduction-rescue-sepcies 

http://www.nytimes.com/2016/07/27/science/dolly-the-sheep-clones.html?_r=0;

https://doi.org/10.1038/nature.2012.10228

 

Organisational / managerial skills

Institutional Responsibilities

2000- Group Leader of 5-12 people (Experimental Embryology Group), including permanent staff, PhD and Post Docs.

2000- to present - Scientific Coordination of large national and international research projects (detail below)

2019 – to present. Leader of the Research Steering Committee of the Faculty of Veterinary Medicine

2019 – to present. Member of the Senate of University of Teramo

2018 - to present. Responsible for undergraduate students with learning problem for the Veterinary Faculty

2010 - to present. Faculty Committee member for the European Association for Education Veterinary medicine (EAEVE)

2000 - to present. Member of the Council of the Faculty of Veterinary Medicine, University of Teramo

 

2001 - to present. Member of the Committee of the PhD programme in Veterinary sciences

 

Commissions of Trust

2009-2011 Scientific Advisory Board, of EU ANIMBIOGEN, VII FP EU - institute of Animal Genetics, Polish Academy of Sciences, Warsaw, Poland 2009-2012,

2007 - present– Review Board member for the European Science Foundation (EFS).

2010-2013 Managing Committee Deputy of the EU COST - “Gemini”, VII FP EU.

2014-2017 Managing Committee Deputy of the EU COST - “Epiconcept”, VII FP EU.

 

Prizes/ Awards/ Academy memberships;

Japanese Society for the Promotion of Science (JSPS) Fellow, Riken Institute, Tsukuba, Japan

Best Scientific Contribution: 2016.03.10 International Symposium on the Future of Nuclear Transfer and Nuclear Reprogramming, Tsukuba, Riken Institute, Japan.

 

Major contributions to the early careers of excellent researchers; - Several of my former PhDs/Post Docs are permanently employed in prestigious research Institutions: Prof. G. Ptak, ERC grantee (Starting Grant) under my guidance, University Jagellonian Krakow, Poland; Dr. Lopes F., University of Edinburgh, UK; Dr. Fidanza A., Medical Research Council, Edinburgh, UK; Dr. Sampino S. Polish Academy of Sciences, Poland; Dr. Iuso D., University of Grenoble, France; Dr. Turco-Yaioy M:, University of Cambridge, UK; Prof. Matsukawa K., University of Kochi, Japan

 

Organization of International Meetings

Scientific Committee International Congress on Animal Reproduction (ICAR), Bologna June 27-2 July 2020 (postponed to 2022, same dates)

 

Scientific Committee Workshop "Micromanipulation (of oocyte/embryo)" Managed jointly with Jan Detterer (Germany). 33 Meeting AETE, Bath, 8-9 September 2017; UK         08/09/2017                

 

Scientific Committee International Conference “Epigenetic Penetrance of Reproductive Technologies”, University of Teramo, 9-10 June 2016, project EU Horizon2020 "EraofArt", Twinn action 09/06/2016, 10/06/2016.

 

Scientific Committee Workshop “Pre-implantation embryos development in large animals: oocytes maturation, fertilization, embryo culture and cryopreservation”, University of Teramo, 11-22 April 2016 EU Horizon2020 "EraofArt", Twinn action                      

                                  

Scientific Committee European Science Foundation Programme “EuroSTELLS”: Workshop “Challenges in Stem Cell Differentiation and Transplantation” http://www.esf.org , 30 September – 3 October 2007, Milan, Italy.        30/09/2007     03/10/2007    

 

Scientific Committee European Science Foundation Programme “EuroSTELLS”: Workshop “Stem Cell Niches” http://www.esf.org/media-centre/press, 10-12 Gennaio 2008, Barcellona, Spain10/01/2008 12/01/2008

 

Editorial Board member

Editorial Board Member Journal “Animal Science Papers and Reports”. ISSN 0860-4037

Editorial Board Member Journal "Cellular Reprogramming". ISSN: 2152-4971

Editorial Board Member of “Journal of Reproduction and Development”, ISSN: 1348-4400

 

Reviewer, ad hoc

Nature, Science, Biology of Reproduction, Theriogenology, Gene, Trends in Biotechnology, Cellular Reprogramming, Theriogenology, Reproduction, Fertility and Development, Gene, Genomics, Reproduction in Domestic Animals, Animal Reproduction Science, Journal of Assisted Reproduction Genetics, PLoS ONE, Frontiers series.

 

Evaluator for funding agencies

2007 –Referee for the European Research Council –remote evaluation - (ERC), VII FP EU

2010-2014 Referee for the Marie Curie Fellowship Programme, VII FP EU

2011-2019 Referee for the Polish Academy of Sciences

2013 Referee for the Royal Society, UK

2013 Referee for the USA Israel Bi-National Scientific Programme

2015-2019 Referee for the Cyprus Academy of Sciences

2017-to present Referee for the European Science Foundation

2018 Referee for the Lithuanian National Science Foundation

2018 Referee for the Medical Research Council, UK

2019 Referee - expert pool -for the Fonds Wetenschappelijk Onderzoek – Vlaanderen (FWO), Belgium

2018-to present Referee for the Dutch Science Foundation

2018-to present Referee for the Czech Academy of Sciences

2010-to present Referee for the Italian Minister of Research and University (MIUR)

2021-to present Referee for the Natural Sciences and Engineering Research Council of Canada

2022-to present Referee for the  French National Research Agency (ANR)

2022-to present Referee for the Spanish National Research Agency.

2025-to present, Referee for the Slovak National Research Agency.

 

Membership of Scientific Societies

Italian Society of Veterinary Sciences,

Italian Association of Veterinary Physiologists

International Embryo Transfer Association (IETS)

Society for Reproduction and Fertility (SRF)

 

Job-related skills

Short Biographical Sketch

I have started to work on embryos 30 years ago as a self-made man in a small Veterinary Faculty in Sardinia, Italy. I have dedicated a great deal of efforts on embryo multiplication by splitting, blastomere separation and embryonic cells nuclear transfer during my PhD. Then I joined Keith Campbell and Ian Wilmut at the Roslin Institute, Edinburgh, Scotland, UK, to work on a sheep nuclear transfer project aiming at cell cycle coordination between enucleated oocytes and donor embryonic nuclei. The publications jointly delivered (Campbell, KHS Loi et al., Reviews of Reproduction, 1996; Campbell KHS, Loi P., Biol Reprod. 1994) are milestones in nuclear transfer research and prepared the ground for the production of the first sheep cloned from embryonic (Campbell et al., Nature 1996;380: 64-6) and next adult cell lines (Wilmut et al., Nature 1997, 385: 810-3). After my work at Roslin, I continued to work on Somatic Cell Nuclear Transfer (SCNT) producing the first ever wild animal by cloning (Loi et al. Nature Biotechnology, 2001). SCNT still remains my primary research interest where I have given significant contributions. I have in fact demonstrated for the first time that non-living cells can generate offspring upon SCNT (Loi et al., Biol Reprod. 2002), a breakthrough that has prepared the ground for cloning lyophilized cells (Loi et al., PloS One 2018), till the most recent attempts to clone the mammoth (Yamagata et al., 2019, Sci Rep. 2019; 9:4050). My commitment to elucidate the mechanisms for nuclear reprogramming after SCNT continues, and the latest outcomes of my research group represent a valid option to improve nuclear reprogramming by conferring somatic cells the nuclear structure of a spermatid cells, by the heterologous expression of protamine1 (Iuso et al., Cell Reports 2015; Czernik et al., Nature Protocols 2016; Palazzese et al., Plos One, 2018).

Metrics - My H Index on Google Scholar is 39, with 5113 citations. Please, note the Veterinary Sciences publications have a lower IF comparing to other branch of science; therefore, my H Index for veterinary research is high.

 

Granted patents:

 

Pasqualino Loi et al., Improved method for reconstructing a non-human animal embryo EP 3134532 A1 (WO2015162170A1), PCT/EP2015/058701

 

Pasqualino Loi & Pietro Cappai. Process for reconstructing a non-human mammalian embryo by nuclear transfer using a heat treated donor nucleus and preparing a non-human mammal US 7064245 B1;PCT/IT1999/000160

 

 

List of Publications

             

1. Czernik M, Palazzese L, Winiarczyk D, Iuso D, Khochbin S, Fulka J, Fulka H, Villafranca R, Rielo JA, Sanchez-Rodriguez A, Latorre N, Agudo-Rios C, Roldan ERS, Teves ME, Loi P Protamine sequence determines species-specific nuclear shape and histone retention.

Science. 2025 Jul 12;28(8):113102.

 

1. Palazzese L, Iuso D, Scudieri A, Moncada M, Lo Sterzo M, Boffa F, Loi P, Czernik M, Gioia L.Impaired calcium oscillations and PLCζ dysfunction as key contributors to the low efficiency of ICSI and artificial activation in sheep. 2025 Oct 15;246:117539.

 

Moncada M, Lo Sterzo M, Palazzese L, Boffa F, Anzalone DA, Iuso D, Czernik M, Gioia L, Turri F, Mislei B, Bucci D, Bonnet J, Colotte M, Tuffet S, Bochenek M, Loi P.Fertility preservation of vacuum-dried ram spermatozoa stored for four years at room temperature.  Theriogenology. 2025 Jun;239:117390.

1. Loi P, Palazzese L, Moncada M, Sterzo ML, Iuso D, Czernik M, Wakayama T, Kikawada T Advances in induced anhydrobiosis for cell and gamete storage. Trends Biotechnol. 2025 Jul;43(7):1599-1608

 

1. Lo Sterzo M, Iuso D, Palazzese L, Moncada M, Boffa F, Scudieri A, Gioia L, Czernik M, Loi P Exogenous LEA proteins expression enhances cold tolerance in mammalian cells by reducing oxidative stress. Sci Rep. 2025 Jan 27;15(1):3351

 

1. Scudieri A, Valbonetti L, Peric T, Cotticelli A, Ramal-Sánchez M, Loi P, Gioia L Autophagy is involved in granulosa cell death and follicular atresia in ewe ovaries.T heriogenology. 2024 Sep 15;226:236-242

 

1. Gioia L, Palazzese L, Czernik M, Iuso D, Fulka H, Fulka J Jr, Loi P. Oocyte activation is a cytoplasm-confined event so far: what about the nucleus? 2024 Feb 2;167(3):e230360

 

1. Palazzese L, Turri F, Anzalone DA, Saragusty J, Bonnet J, Colotte M, Tuffet S, Pizzi F, Luciani A, Matsukawa K, Czernik M, Loi P Reviving vacuum-dried encapsulated ram spermatozoa via ICSI after 2 years of storage. Front Vet Sci. 2023 Nov 30;10:1270266.

 

 

1. Palazzese L, Czernik M, Matsukawa K,Loi PSomatic Cell Nuclear Transfer Using Freeze-Dried Protaminized Donor Nuclei.  Methods Mol Biol. 2023;2647:211-224

 

1. Fulka H,Loi P, Czernik M, Surani A, Fulka JOmne vivum ex ovo: the oocyte reprogramming and remodeling activities. 2023 Jan 12;165(3):R75-R89

 

 

1. Thiangthientham P, Kallayanathum W, Anakkul N, Suwimonteerabutr J, Santiviparat S, Techakumphu M,Loi P, Tharasanit Effects of freeze-drying on the quality and fertilising ability of goat sperm recovered from different parts of the epididymis. 2023 Jan 1;195:31-39.

 

1. Czernik M, Winiarczyk D, Sampino S, Gręda P, Parillo S, Modliński JA,Loi PMitochondrial function and intracellular distribution is severely affected in in vitro cultured mouse embryos. Sci Rep. 2022 Sep 27;12(1):16152. doi: 10.1038/s41598-022-20374-6

 

1. Capra E, Toschi P, Del Corvo M, Lazzari B, Stella A, Williams JL,Loi P, Ajmone Marsan P.Maternal undernutrition during periconceptional period affects whole-genome ovine muscle methylation in adult offspring. J Anim Sci. 2022 Aug 1;100(8):skac180

 

 

1. Fulka H,Loi P, Palazzese L, Benc M, Fulka Jr J JrNucleus reprogramming/remodeling through selective enucleation (SE) of immature oocytes and zygotes: a nucleolus point of view.J Reprod Dev. 2022 Jun 1;68(3):165-172.

 

1. Comizzoli P, Loi P, Patrizio P, Hubel A. Long-term storage of gametes and gonadal tissues at room temperatures: the end of the ice age? J Assist Reprod Genet. 2022 Feb;39(2):321-325

 

1. Fulka H, Loi P, Palazzese L, Benc M, Fulka Jr J Jr Nucleus reprogramming/remodeling through selective enucleation (SE) of immature oocytes and zygotes: a nucleolus point of view. J Reprod Dev. 2022 Jun 1;68(3):165-172

 

1. Anzalone DA, Palazzese L, Czernik M, Sabatucci A, Valbonetti L, Capra E, Loi P Controlled spermatozoa-oocyte interaction improves embryo quality in sheep. Sci Rep. 2021 Nov 19;11(1):22629

 

1. Hildebrandt TB, Hermes R, Goeritz F, Appeltant R, Colleoni S, de Mori B, Diecke S, Drukker M, Galli C, Hayashi K, Lazzari G, Loi P, Payne J, Renfree M, Seet S, Stejskal J, Swegen A, Williams SA, Zainuddin ZZ, Holtze S. The ART of bringing extinction to a freeze - History and future of species conservation, exemplified by rhinos. 2021 Jul 15;169:76-88.

 

1. Capra E, Toschi P, Del Corvo M, Lazzari B, Stella A, Williams JL, Loi P, Ajmone Marsan P Short Communication: Maternal undernutrition during peri-conceptional period affects whole genome ovine muscle methylation in adult offspring. J Anim Sci. 2022 May 17:skac180. doi: 10.1093/jas/skac180

 

1. Dahlen CR, Borowicz PP, Ward AK, Caton JS, Czernik M, Palazzese L, Loi P, Reynolds LP Programming of Embryonic Development. Int J Mol Sci. 2021 Oct 28;22(21):11668

 

1. Rychtarova J, Langerova A, Fulka H, Loi P, Benc M, Fulka J Jr Interspecific ICSI for the Assessment of Sperm DNA Damage: Technology Report. Animals (Basel). 2021 Apr 26;11(5):1250

 

1. Loi P, Palazzese L, Scapolo PA, Fulka J, Fulka H, Czernik M 25th ANNIVERSARY OF CLONING BY SOMATIC-CELL NUCLEAR TRANSFER: Scientific and technological approaches to improve SCNT efficiency in farm animals and pets. 2021 Jun 11;162(1):F33-F43

 

1. Ressaissi Y, Anzalone DA, Palazzese L, Czernik M, Loi P The impaired development of sheep ICSI derived embryos is not related to centriole dysfunction. 2021 Jan 1;159:7-12

 

1. Palazzese L, Anzalone DA, Turri F, Faieta M, Donnadio A, Pizzi F, Pittia P, Matsukawa K, Loi P Whole genome integrity and enhanced developmental potential in ram freeze-dried spermatozoa at mild sub-zero temperature. Sci Rep. 2020 Nov 2;10(1):18873

 

1. Helena Fulka, Jana Rychtarova & Loi P. The nucleolus-like and precursor bodies of mammalian oocytes and 1 embryos and their possible role in post-fertilisation centromere remodelling.  Biochemical Society Transactions, 2020, pii: BST20190847. doi: 10.1042/BST20190847

 

1. Czernik M, Fidanza A, Luongo FP, Valbonetti L, Scapolo PA, Patrizio P,Loi P. Late Embryogenesis Abundant (LEA) proteins confer water stress tolerance to mammalian somatic cells. Cryobiology. 2020 Jan 14. pii: S0011-2240(19)30232-9

 

1. Saragusty J, Anzalone DA, Palazzese L, Arav A, Patrizio P, Gosálvez J,Loi P.  Dry biobanking as a conservation tool in the Anthropocene. Theriogenology. 2020 Jan 13. pii: S0093-691X(20)30028-5.

 

1. Toschi P, Capra E, Anzalone DA, Lazzari B, Turri F, Pizzi F, Scapolo PA, Stella A, Williams JL, Ajmone Marsan P,Loi P. Maternal peri-conceptional undernourishment perturbs offspring sperm methylome. Reproduction. 2020 May;159(5):513-523

 

1. Marta Czernik, Debora A. Anzalone, Luca Palazzese, Mami Oikawa, Pasqualino Loi (2019). Somatic cell nuclear transfer: failures, successes and the challenges ahead. THE INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, ISSN: 0214-6282, doi: 10.1387/ijdb.180324mc

 

1. Fulka H, Ogura A,Loi P, Fulka Jr J Jr.  Dissecting the role of the germinal vesicle nuclear envelope and soluble content in the process of somatic cell remodelling and reprogramming. J Reprod Dev. 2019 Oct 23;65(5):433-441

 

1. Saragusty J,Loi P.  Exploring dry storage as an alternative biobanking strategy inspired by Nature. Theriogenology. 2019 Mar 1;126:17-27

 

1. Fulka JJ, Benc M,Loi P, Langerova A, Fulka H. Function of atypical mammalian oocyte/zygote nucleoli and its implications for reproductive biology and medicine. Int J Dev Biol. 2019;63(3-4-5):105-112

 

1. Luca Palazzese, Jaime Gosálvez, Debora A. Anzalone, Pasqualino Loi, Joseph Saragusty (2018). DNA fragmentation in epididymal freeze-dried ram spermatozoa impairs embryo development. JOURNAL OF REPRODUCTION AND DEVELOPMENT, ISSN: 0916-8818, doi: 10.1262/jrd.2018-033

 

1. Debora Agata Anzalone, Luca Palazzese, Domenico Iuso, Giuseppe Martino, Pasqualino Loi (2018). Freeze-dried spermatozoa: An alternative biobanking option for endangered species. ANIMAL REPRODUCTION SCIENCE, vol. 190, p. 85-93, ISSN: 0378-4320, doi: 10.1016/j.anireprosci.2018.01.010
2. Loi P, Galli C, Lazzari G, Matsukawa K, Fulka J Jr, Goeritz F, Hildebrandt TB. Development to term of sheep embryos reconstructed after inner cell mass/trophoblast exchange. J Reprod Dev. 2018 Apr 13;64(2):187-191

 

1. Hildebrandt TB, Hermes R, Colleoni S, Diecke S, Holtze S, Renfree MB, Stejskal J, Hayashi K, Drukker M,Loi P, Göritz F, Lazzari G, Galli C. Embryos and embryonic stem cells from the white rhinoceros. Nat Commun. 2018 Jul 4;9(1):2589.

 

1. Luca Palazzese, Marta Czernik, Domenico Iuso, Paola Toschi, Pasqualino Loi (2018). Nuclear quiescence and histone hyper-acetylation jointly improve protamine-mediated nuclear remodeling in sheep fibroblasts. PLOS ONE, vol. 13, ISSN: 1932-6203, doi: 10.1371/journal.pone.0193954

 

1. LOI, Pasqualino, IUSO, DOMENICO, TOSCHI, PAOLA, PALAZZESE, LUCA, CZERNIK, Marta Teresa (2017). Alternative strategies for nuclear reprogramming in somatic cell nuclear transfer (SCNT). ANIMAL REPRODUCTION, vol. 14, p. 377-382, ISSN: 1806-9614, doi: 10.21451/1984-3143-AR915

 

1. Capra E., Toschi P., Del Corvo M. D., Lazzari B., Scapolo P. A., Loi P., Williams J. L., Stella A., Ajmone-Marsan P. (2017). Genome-wide epigenetic characterization of tissues from three germ layers isolated from sheep fetuses. FRONTIERS IN GENETICS, vol. 8, 115, ISSN: 1664-8021, doi: 10.3389/fgene.2017.00115

 

1. Loi Pasqualino, Iuso Domenico, Czernik Marta, Ogura Atsuo (2016). A New, Dynamic Era for Somatic Cell Nuclear Transfer?. TRENDS IN BIOTECHNOLOGY, vol. 34, p. 791-797, ISSN: 0167-7799, doi: 10.1016/j.tibtech.2016.03.008

 

1. Toschi Paola, Czernik Marta, Zacchini Federica, Fidanza Antonella, Loi Pasqualino, Ptak Grazyna Ewa (2016). Evidence of placental autophagy during early pregnancy after transfer of in vitro produced (IVP) sheep embryos. PLOS ONE, vol. 11, e0157594, ISSN: 1932-6203, doi: 10.1371/journal.pone.0157594

 

 

1. ANZALONE, DEBORA AGATA, IUSO, DOMENICO, CZERNIK, Marta Teresa, PTAK, Grazyna, LOI, Pasqualino (2016). Plasma membrane and acrosome loss before ICSI is required for sheep embryonic development. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, vol. 33, p. 757-763, ISSN: 1573-7330, doi: 10.1007/s10815-016-0709-1

 

1. Czernik Marta, Iuso Domenico, Toschi Paola, Khochbin Saadi, Loi Pasqualino (2016). Remodeling somatic nuclei via exogenous expression of protamine 1 to create spermatid-like structures for somatic nuclear transfer. NATURE PROTOCOLS, vol. 11, p. 2170-2188, ISSN: 1754-2189, doi: 10.1038/nprot.2016.130

 

 

1. Saragusty Joseph, Diecke Sebastian, Drukker Micha, Durrant Barbara, Friedrich Ben-Nun Inbar, Galli Cesare, Göritz Frank, Hayashi Katsuhiko, Hermes Robert, Holtze Susanne, Johnson Stacey, Lazzari Giovanna, Loi Pasqualino, Loring Jeanne F., Okita Keisuke, Renfree Marilyn B., Seet Steven, Voracek Thomas, Stejskal Jan, Ryder Oliver A., Hildebrandt Thomas B (2016). Rewinding the process of mammalian extinction. ZOO BIOLOGY, vol. 35, p. 280-292, ISSN: 0733-3188, doi: 10.1002/zoo.21284

 

1. Loi Pasqualino, Toschi Paola, Zacchini Federica, Ptak Grazyna, Scapolo Pier A., Capra Emanuele, Stella Alessandra, Marsan Paolo Ajmone, Williams John L. (2016). Synergies between assisted reproduction technologies and functional genomics. GENETICS SELECTION EVOLUTION, vol. 48, 48, ISSN: 0999-193X, doi: 10.1186/s12711-016-0231-z

 

1. Iuso Domenico, Czernik Marta, Toschi Paola, Fidanza Antonella, Zacchini Federica, Feil Robert, Curtet Sandrine, Buchou Thierry, Shiota Hitoshi, Khochbin Saadi, Ptak Grazyna Ewa, Loi Pasqualino (2015). Exogenous Expression of Human Protamine 1 (hPrm1) Remodels Fibroblast Nuclei into Spermatid-like Structures. CELL REPORTS, vol. 13, p. 1765-71-1771, ISSN: 2211-1247, doi: 10.1016/j.celrep.2015.10.066

 

1. Cree L, Loi P (2015). Mitochondrial replacement: from basic research to assisted reproductive technology portfolio tool-technicalities and possible risks. MOLECULAR HUMAN REPRODUCTION, vol. 21, ISSN: 1360-9947, doi: 10.1093/molehr/gau082

 

1. Ptak Grazyna E, Toschi Paola, Fidanza Antonella, Czernik Marta, Zacchini Federica, Modlinski Jacek A, Loi Pasqualino (2014). Autophagy and apoptosis: parent-of-origin genome-dependent mechanisms of cellular self-destruction. OPEN BIOLOGY, vol. 4, ISSN: 2046-2441, doi: 10.1098/rsob.140027

 

1. S Sampino, G R Juszczak, F Zacchini, A H Swiergiel, J A Modlinski, P Loi, G E Ptak (2014). Grand-paternal age and the development of autism-like symptoms in mice progeny. TRANSLATIONAL PSYCHIATRY, vol. 4, ISSN: 2158-3188, doi: 10.1038/tp.2014.27

 

1. Fidanza A, Toschi P, Zacchini F, Czernik M, Palmieri C, Scapolo Pa, Modlinski Ja, Loi P, Ptak G (2014). Impaired placental vasculogenesis compromises the growth of sheep embryos developed in vitro. BIOLOGY OF REPRODUCTION, vol. 91, ISSN: 0006-3363, doi: 10.1095/biolreprod.113.113902

 

1. Iuso D, Czernik M, Zacchini F, Ptak G, Loi P (2013). A simplified approach for oocyte enucleation in mammalian cloning.. CELLULAR REPROGRAMMING, ISSN: 2152-4971, doi: 10.1089/cell.2013.0051

 

1. Ptak GE, Modlinski JA, Loi P (2013). Embryonic diapause in humans: time to consider?. REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, ISSN: 1477-7827, doi: 10.1186/1477-7827-11-92

 

1. Iuso D, Czernik M, Di Egidio F, Sampino S, Zacchini F, Bochenek M, Smorag Z, Modlinski JA, Ptak G, Loi P (2013). Genomic stability of lyophilized sheep somatic cells before and after nuclear transfer.. PLOS ONE, ISSN: 1932-6203, doi: 10.1371/journal.pone.0051317

 

1. Loi P, Czernik M, Zacchini F, Iuso D, Scapolo PA, Ptak G (2013). Sheep: the first large animal model in nuclear transfer research.. CELLULAR REPROGRAMMING, ISSN: 2152-4971, doi: 10.1089/cell.2013.0032

 

1. Fulka J Jr, Langerova A, Loi P, Ptak G, Albertini D, Fulka H (2013). The ups and downs of somatic cell nucleus transfer (SCNT) in humans.. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, ISSN: 1058-0468, doi: 10.1007/s10815-013-0053-7

 

1. Loi, D. Iuso, M. Czernik, F. Zacchini, G. Ptak (2013). Towards storage of cells and gametes in dry form. TRENDS IN BIOTECHNOLOGY, vol. 31, p. 688-695, ISSN: 0167-7799, doi: 10.1016/j.tibtech.2013.09.004

 

1. Czernik M, Fidanza A, Sardi M, Galli C, Brunetti D, Malatesta D, Della Salda L, Matsukawa K, Ptak G, Loi P (2013). Differentiation potential and GFP labeling of sheep bone marrow derived mesenchymal stem cells.. JOURNAL OF CELLULAR BIOCHEMISTRY, ISSN: 0730-2312, doi: 10.1002/jcb.24310.

 

1. Iuso D, Czernik M, Di Egidio F, Sampino S, Zacchini F, Bochenek M, Smorag Z, Modlinski JA, Ptak G, Loi P (2013). Genomic stability of lyophilized sheep somatic cells before and after nuclear transfer. PLOS ONE, vol. Epub 2013 Jan 8., p. 1-10, ISSN: 1932-6203, doi: 10.1371/journal.pone.0051317

 

1. Ptak GE, D'Agostino A, Toschi P, Fidanza A, Zacchini F, Czernik M, Monaco F, Loi P (2013). Post-implantation mortality of in vitro produced embryos is associated with DNA methyltransferase 1 dysfunction in sheep placenta. HUMAN REPRODUCTION, vol. 28, p. 298-305, ISSN: 0268-1161, doi: doi: 10.1093/humrep/des397

 

1. Ptak G, Zacchini F, Czernik M, Fidanza A, Palmieri C, Della Salda L, Scapolo PA, Loi P (2012). A short exposure to polychlorinated biphenyls deregulates cellular autophagy in mammalian blastocyst in vitro. . HUMAN REPRODUCTION, vol. 27, p. 1034-1042, ISSN: 0268-1161, doi: doi: 10.1093/humrep/der454

 

1. Ptak GE, Tacconi E, Czernik M, Toschi P, Modlinski JA, LOI P (2012). Embryonic diapause is conserved across mammals.. PLOS ONE, vol. 7, p. 1-7, ISSN: 1932-6203, doi: http://dx.crossref.org/10.1371%2Fjournal.pone.0033027

 

1. Loi P, Teruhiko Wakayama, Josef Fulka Jr and Grazyna Ptak. (2011). Biological time machines: a realistic approach for cloning an extinct mammal.. ENDANGERED SPECIES RESEARCH, vol. Vol. 14: , p. 227-233, ISSN: 1613-4796

 

1. Zacchini F, Czernik M, Iuso D, Toschi P, di Egidio F, Scapolo PA, Loi P, Ptak G. (2011). Efficient production and cellular characterization of sheep androgenetic embryos. . CELLULAR REPROGRAMMING, vol. Dec;13(6), p. 495-502, ISSN: 2152-4971

 

1. Loi P, Fulka J Jr, Hildebrand T, Ptak G. (2011). Genome of non-living cells: trash or recycle? . REPRODUCTION, vol. Oct;142(4), p. 497-503, ISSN: 1470-1626

 

 

1. Loi P, Modlinski JA, Ptak G. (2011). Interspecies somatic cell nuclear transfer: a salvage tool seeking first aid. . THERIOGENOLOGY, vol. Jul 15;76(2), p. 217-228, ISSN: 0093-691

 

1. Fulka J Jr, Langerova A, Loi P, Martinkova S, Fulka H. (2011). Transplantation of nucleoli into human zygotes: not as simple as expected? . JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, vol. 28(5), p. 385, ISSN: 1058-0468

 

1. FULKA JR J, LOI P, PTAK G, FULKA H, JOHN JS (2009). Hope for the Mammoth?. CLONING AND STEM CELLS, ISSN: 1536-2302

 

1. LOI P, BEAUJEAN N, KHOCHBIN S, FULKA J JR, PTAK G (2008). Asymmetric nuclear reprogramming in somatic cell nuclear transfer?. BIOESSAYS, vol. 30, p. 66-74, ISSN: 0265-9247

 

1. FULKA J JR, FULKA H, ST JOHN J, GALLI C, LAZZARI G, LAGUTINA I, FULKA J, LOI P (2008). Cybrid human embryos--warranting opportunities to augment embryonic stem cell research. TRENDS IN BIOTECHNOLOGY, vol. 26, p. 469-474, ISSN: 0167-779

 

1. LOI P, MATSUKAWA K, PTAK G, CLINTON M, FULKA J JR, NATHAN Y, ARAV A (2008). Freeze-dried somatic cells direct embryonic development after nuclear transfer. PLOS ONE, vol. e2978., ISSN: 1932-6203

 

 

1. TURCO MY, MATSUKAWA K, CZERNIK M, GASPERI V, BATTISTA N, DELLA SALDA L, SCAPOLO PA, LOI P, MACCARRONE M, PTAK G (2008). High levels of anandamide, an endogenous cannabinoid, block the growth of sheep preimplantation embryos by inducing apoptosis and reversible arrest of cell proliferation. HUMAN REPRODUCTION, vol. 23, p.

 

1. LOI P, KAZUTSUGU MATSUKAWA SEIYA TAKAHASHI, SATOSHI AKAGI GRAZYNA PTAK (2008). Nuclear reprogramming: what has been done and potential avenues for improvements. CENTRAL EUROPEAN JOURNAL OF BIOLOGY, vol. 3, p. 211-223, ISSN: 1895-104X

 

 

1. LOI P, MATZUKAWA K, PTAK G, NATAN Y, FULKA J JR, ARAV A (2008). Nuclear transfer of freeze-dried somatic cells into enucleated sheep oocytes. REPRODUCTION IN DOMESTIC ANIMALS, vol. 43, p. 417-422, ISSN: 0936-6768

 

 

1. PALMIERI C, LOI P, PTAK G, DELLA SALDA L (2008). Review paper: a review of the pathology of abnormal placentae of somatic cell nuclear transfer clone pregnancies in cattle, sheep, and mice. VETERINARY PATHOLOGY, vol. 45, p. 865-880, ISSN: 0300-9858

 

 

1. LOI P, GALLI C, PTAK G (2007). Cloning of endangered mammalian species: any progress?. TRENDS IN BIOTECHNOLOGY, vol. 25, p. 195-200, ISSN: 0167-7799

 

1. Matsukawa K, Turco MY, Scapolo PA, Reynolds L, Ptak G, Loi P (2007). Development of sheep androgenetic embryos is boosted following transfer of male pronuclei into androgenetic hemizygotes. . CLONING AND STEM CELLS, vol. 9, p. 374-381, ISSN: 1536-2302

 

 

1. PALMIERI C, LOI P, REYNOLDS LP, PTAK G, DELLA SALDA L (2007). Placental abnormalities in ovine somatic cell clones at term: a light and electron microscopic investigation. PLACENTA, vol. 28, p. 577-584, ISSN: 0143-4004

 

 

1. PTAK G, MATSUKAWA K, PALMIERI C, DELLA SALDA L, SCAPOLO PA, LOI P (2006). Developmental and functional evidence of nuclear immaturity in prepubertal oocytes. HUMAN REPRODUCTION, vol. 21, p. 2228-2237, ISSN: 0268-1161

 

 

1. GRAZYNA PTAK, FEDERICA LOPES, KAZUTSUGU MATSUKAWA, MARIAN TISCHNER, PASQUALINO LOI (2006). Leukemia Inhibitory Factor enhances sheep ferilization in vitro via an influence on the oocyte. THERIOGENOLOGY, p. 1-10, ISSN: 0093-691X

 

 

1. LOI P, CLINTON, M., VACKOVA, I., FULKA JR., J., FEIL, R., PALMIERI, DELLA SALDA, L., AND PTAK G (2006). Placental abnormalities associated with post-natal mortality in sheep somatic cell clones. THERIOGENOLOGY, vol. 65 , p. 1110-1121, ISSN: 0093-691X, doi: doi:10.1016/j.theriogenology.2005.07.016

 

1. IRENA VACKOVA, RADOMIR KREN, PASQUALINO LOI, VLADIMR KRYLOV AND JOSEF FULKA, LOI P (2006). The absence of DNA replication checkpoint in porcine zygotes. ZYGOTE, vol. 14, p. 33-37, ISSN: 0967-1994

 

1. FULKA J JR, MRAZEK M, FULKA H, LOI P (2005). Mammalian oocyte therapies. CLONING AND STEM CELLS, vol. 7, p. 183-188, ISSN: 1536-2302

 

 

1. LOI P (2004). Are there realistic opportunities to apply cloning and the other reproductive biotechnologies for safeguarding endangered species?. ANIMAL SCIENCE PAPERS AND REPORTS, vol. 22, p. 81-89, ISSN: 0860-4037

 

1. FULKA J JR, LOI P, FULKA H, PTAK G, NAGAI T. (2004). Nucleus transfer in mammals: noninvasive approaches for the preparation of cytoplasts. TRENDS IN BIOTECHNOLOGY, vol. 22, p. 27-283, ISSN: 0167-7799

 

1. MATTIOLI M, GIOIA L, TURRIANI M, CAPACCHIETTI G, LOI P, PTAK G. (2004). Oocyte maturation is required for correct sperm chromatin rearrangement. VETERINARY RESEARCH COMMUNICATIONS, vol. 28, p. 205-208, ISSN: 0165-7380

 

1. LOI P, DELLA SALDA L., PTAK G. (2004). Peri and post natal mortality of somatic cell clones in sheep. ANIMAL SCIENCE PAPERS AND REPORTS, vol. 22, suppl. 1, p. 59-70, ISSN: 0860-4037

 

1. LOI P, DELLA SALDA, L., PTAK G. (2004). Peri- and post-natal mortality of somatic cell clones in sheep. ANIMAL SCIENCE PAPERS AND REPORTS, vol. 22, p. 59-63, ISSN: 0860-4037

 

1. BEAUJEAN N, TAYLOR JE, MCGARRY M, GARDNER JO, WILMUT I, LOI P, PTAK G, GALLI C, LAZZARI G, BIRD A, YOUNG LE, MEEHAN RR (2004). The effect of interspecific oocytes on demethylation of sperm DNA. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 20, p. 7636-7640, ISSN: 1091-6490

 

1. LOI P, FULKA J JR, PTAK G (2003). Amphibian and mammal somatic-cell cloning: different species, common results? (Cover page). TRENDS IN BIOTECHNOLOGY, vol. 21, p. 471-473, ISSN: 0167-7799

 

1. MATTIOLI M, BARBONI B, GIOIA L, LOI P, LUISA G (2003). Cold-induced calcium elevation triggers DNA fragmentation in immature pig oocytes. MOLECULAR REPRODUCTION AND DEVELOPMENT, vol. 65, p. 289-297, ISSN: 1040-452X

 

1. YOUNG LE, SCHNIEKE AE, MCCREATH KJ, WIECKOWSKI S, KONFORTOVA G, FERNANDES K, PTAK G, KIND AJ, WILMUT I, LOI P, FEIL R. (2003). Conservation of IGF2-H19 and IGF2R imprinting in sheep: effects of somatic cell nuclear transfer. MECHANISMS OF DEVELOPMENT, vol. 120, p. 1433-1442, ISSN: 0925-4773

 

1. PTAK GRAZYNA, TISCHENR MARIAN, BERNABÒ NICOLA, LOI P (2003). Donor-Dependent Developmental Competence of Oocytes from Lambs Subjected to Repeated Hormonal Stimulation. BIOLOGY OF REPRODUCTION, vol. 2003 Feb 5, ISSN: 0006-3363

 

 

1. FULKA J, MOOR RM, LOI P, FULKA J (2003). Enucleolation of porcine oocytes. THERIOGENOLOGY, vol. 59, p. 1879-1885, ISSN: 0093-691X

 

 

1. FULKA J JR, LOI P, FULKA H, KREN R, DEAN W, MRAZEK M, REIK W (2003). Nucleus replacement in Mammalian oocytes. CLONING AND STEM CELLS, vol. 4, p. 181-187, ISSN: 1536-2302

 

1. LOI P, BARBONI B, PTAK G (2002). Cloning advances and challenges for conservation. TRENDS IN BIOTECHNOLOGY, vol. 20, p. 233, ISSN: 0167-7799

 

1. PTAK GRAZYNA, CLINTON MICHAEL, TISHNER MARIAN, MATTIOLI MAURO, BARBONI BARBARA, LOI P (2002). Improving delivery and offspring viability of in vitro-produced and cloned sheep embryos. BIOLOGY OF REPRODUCTION, vol. 67, p. 1719-1725, ISSN: 0006-3363

 

1. LOI P, CLINTON M., BARBONI B., FULKA J. JR., CAPPAI P., FEIL R., MOOR R.H.M., PTAK G. (2002). Nuclei of nonviable ovine somatic cells develop into lambs after nuclear transplantation. BIOLOGY OF REPRODUCTION, vol. 67, p. 442-450, ISSN: 0006-3363

 

1. PTAK G., CLINTON M., BARBONI B., MUZZEDDU M., CAPPAI P., TISCHNER M., LOI P (2002). Preservation of the wild European mouflon: the first example of genetic management using a complete program of reproductive biotechnologies. BIOLOGY OF REPRODUCTION, vol. 66, p. 796-801, ISSN: 0006-3363

 

 

1. LOI P, PTAK GRAZYNA, BARBONI BARBARA, FULKA JOSEF JR, CAPPAI PIETRO, MICHAEL CLINTON (2001). Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells. NATURE BIOTECHNOLOGY, vol. 19, p. 962-964, ISSN: 1087-0156

 

 

1. FULKA J. JR, LOI P, LEDDA S, MOOR RHM, FULKA J (2001). Nucleus transfer in mammals: how the oocyte cytoplasm modifies the transferred nucleus. THERIOGENOLOGY, vol. 55, p. 1373-1380, ISSN: 0093-691X

 

1. FULKA JR, J. TESARIK, LOI P, R.M. MOOR (2000). Manipulating the Human embryo: Cell Cycle Checkpoint Controls. CLONING AND STEM CELLS, vol. 2, p. 1-7, ISSN: 1536-2302

 

 

1. LOI P, FULKA J.JR., NAITANA S., CAPPAI P. (1999). Cloning by nuclear transfer: experiences in sheep. LIVESTOCK PRODUCTION SCIENCE, vol. 60, p. 281-294, ISSN: 0301-6226

 

1. PTAK G, LOI P, DATTENA M, TISCHNER M, CAPPAI P (1999). Offspring from one-month-old lambs: studies on the developmental capability of prepubertal oocytes. BIOLOGY OF REPRODUCTION, vol. 61, p. 1568-1574, ISSN: 0006-336

 

 

1. PTAK G., DATTENA M., LOI P, TISCHNER M., CAPPAI P (1999). Ovum pick-up in sheep: efficiency of in vitro embryo production, vitrification and offspring. THERIOGENOLOGY, vol. 52, p. 1105-1114, ISSN: 0093-691X

 

1. LOI P, PTAK G., DATTENA M., LEDDA S., NAITANA S., CAPPAI P (1998). Embryo transfer and related technologies in sheep reproduction. REPRODUCTION NUTRITION DEVELOPMENT, vol. 38, p. 615-628, ISSN: 0926-5287

 

1. FULKA J JR, FIRST NL, LOI P, MOOR RHM (1998). Cloning by somatic cell nuclear transfer. BIOESSAYS, vol. 20, p. 847-851, ISSN: 0265-9247

 

1. LOI P, LEDDA S., FULKA J.JR., CAPPAI P., MOOR R.M. (1998). Development of parthenogenetic and cloned ovine embryos: effect of activation protocols. BIOLOGY OF REPRODUCTION, vol. 58, p. 1177-1187, ISSN: 0006-3363

 

1. FEIL R, KOSHLA S, CAPPAI P, LOI P (1998). Genomic imprinting in ruminants: allele-specific gene expression in parthenogenetic sheep. MAMMALIAN GENOME, vol. 9, p. 831-834, ISSN: 0938-8990

 

1. NAITANA S., LEDDA S., LEONI G., BOGLIOLO L., LOI P, CAPPAI P (1998). Membrane integrity and fertilizing potential of cryopreserved spermatozoa in European mouflon. ANIMAL REPRODUCTION SCIENCE, vol. 52, p. 105-112, ISSN: 0378-4320

 

 

1. LEDDA S, BOGLIOLO L, LEONI G, LOI P, CAPPAI P, NAITANA S (1998). Two culture systems showing a biphasic effect on ovine embryo development from the 1-2 cell stage to hatched blastocysts. REPRODUCTION NUTRITION DEVELOPMENT, vol. 35, p. 629-637, ISSN: 0926-5287

 

1. CAMPBELL K.H.S., LOI P, OTAEGUI P., WILMUT I (1997). Cell cycle co-ordination in embryo cloning by nuclear transfer. JOURNAL OF REPRODUCTION AND FERTILITY, vol. 1, p. 40-46, ISSN: 0022-4251

 

1. LOI P, BOYAZOGLU S., GALLUS M., BRANCA A., LEDDA S., NAITANA S., WILMUT I., CAPPAI P (1997). Embryo Cloning in Sheep: work in progress. THERIOGENOLOGY, vol. 48, p. 1-10, ISSN: 0093-691X

 

1. NAITANA S, LEDDA S, LOI P, LEONI G, BOGLIOLO L, DATTENA M, CAPPAI P (1997). Polyvinyl alcohol as a defined substitute for serum in vitrification and warming solutions to cryopreserve ovine embryos at different stages of development. ANIMAL REPRODUCTION SCIENCE, vol. 48, p. 247-256, ISSN: 0378-4320

 

1. NAITANA S, LEDDA S, LOI P, LEONI G, BOGLIOLO L, DATTENA M, CAPPAI P (1997). Polyvinyl alcohol as a definite substitute for serum in vitrification and warming solutions to cryopreserve ovine embryos at different stages of development. ANIMAL REPRODUCTION SCIENCE, vol. 48, p. 247-256, ISSN: 0378-4320

 

1. CAMPBELL KH, LOI P, OTAEGUI PJ, WILMUT I (1996). Cell cycle co-ordination in embryo cloning by nuclear transfer. REVIEWS OF REPRODUCTION, vol. 1, p. 40-46, ISSN: 1359-6004

 

1. NAITANA S, LOI P, LEDDA S, CAPPAI P, DATTENA M, BOGLIOLO L, LEONI G (1996). Effect of biopsy and vitrification on in vitro survival of ovine embryos at different stages of development. THERIOGENOLOGY, vol. 46, p. 813-824, ISSN: 0093-691X

 

1. LEDDA S., LOI P, BOGLIOLO L., MOOR R.M. FULKA J. JR. (1996). The effect of 6- Dimethylaminopurine (6-DMAP) on DNA synthesis in activated mammalian oocytes. ZYGOTE, vol. 4, p. 7-9, ISSN: 0967-1994

 

 

1. CAMPBELL K.H.S., LOI P, CAPPAI P., WILMUT I (1994). Improved development to blastocyst of ovine nuclear transfer embryos reconstructed during the presumptive S-Phase of enucleated-activated oocytes. BIOLOGY OF REPRODUCTION, vol. 50, p. 1385-1393, ISSN: 0006-3363

 

 

 

 

Research Support

Ongoing Research Support

 

Project Title

 

Funding source

 

Amount

(Euros)

 

Period

 

Role

 

Aims

Drynet

Horizon 2020, No 734434 MSCA Action “RISE”,

1250000

2017-2021

Coordinator

Exploring dry storage of cells, gametes and embryos

Completed Research Support

Coordinator, EU, Horizon2020, MSCA SE, WhyNotDry, 670000 euros, 2023-2027

Coordinator, EU Horizon2020, MSCA-IF-2’16, “Drystore” GA 749537 188000 euro    2017-2019, Development of drying protocols for gametes and cells

PI EU Horizon 2020, Twinning action,  “EraofArt”, GA 698165   295000 euro   2016-2019      Beneficiary Partner    Contemplates major embryo Biotechnologies

PI European Science Foundation grant "Stellar" EUROCORES Programme EuroSTELLS, supported by FP VI EU contract no. ERAS-CT-2003-980409; 154000 euro 2007-2011

PI United States Department of Agriculture (USDA) "Proposal#2007-01215 "" 350.000 $, 01/01/2005/01/2008

PI FP7 EU KBBE-2009-3: Next generation methods to preserve farm animal biodiversity by optimizing present and future breeding options. 130000 euro.

 

Plus I have coordinated other National Grants (projects  Prin [n° 4, excellent research) and Firb [n° 2, basic research] from the Italian Minister for University and Education - MIUR), N° 6, with a total budget of about 5.000.000 euros, and grant supported by private (Bank Foundations N° 2, 150000 euro total budget)

 

 

Digital skills

Self-assessment grid

 

Information processing

communication

Content creation

Safety

Problem solving

Proficient user

Independent user

Basic user

Basic user

Basic user

 

Teramo, 27.08.2025

Yours Faithfully

 

 

 

 

1. List of Publications

                

   1. Czernik M, Palazzese L, Winiarczyk D, Iuso D, Khochbin S, Fulka J, Fulka H, Villafranca R, Rielo JA, Sanchez-Rodriguez A, Latorre N, Agudo-Rios C, Roldan ERS, Teves ME, Loi P Protamine sequence determines species-specific nuclear shape and histone retention. iScience. 2025 Jul 12;28(8):113102.

    

   1. Palazzese L, Iuso D, Scudieri A, Moncada M, Lo Sterzo M, Boffa F, Loi P, Czernik M, Gioia L.Impaired calcium oscillations and PLCζ dysfunction as key contributors to the low efficiency of ICSI and artificial activation in sheep. 2025 Oct 15;246:117539.

    

   1. Moncada M, Lo Sterzo M, Palazzese L, Boffa F, Anzalone DA, Iuso D, Czernik M, Gioia L, Turri F, Mislei B, Bucci D, Bonnet J, Colotte M, Tuffet S, Bochenek M, Loi P.Fertility preservation of vacuum-dried ram spermatozoa stored for four years at room temperature.  Theriogenology. 2025 Jun;239:117390.
   1. Loi P, Palazzese L, Moncada M, Sterzo ML, Iuso D, Czernik M, Wakayama T, Kikawada T Advances in induced anhydrobiosis for cell and gamete storage. Trends Biotechnol. 2025 Jul;43(7):1599-1608

    

   1. Lo Sterzo M, Iuso D, Palazzese L, Moncada M, Boffa F, Scudieri A, Gioia L, Czernik M, Loi P Exogenous LEA proteins expression enhances cold tolerance in mammalian cells by reducing oxidative stress. Sci Rep. 2025 Jan 27;15(1):3351

    

   1. Scudieri A, Valbonetti L, Peric T, Cotticelli A, Ramal-Sánchez M, Loi P, Gioia L Autophagy is involved in granulosa cell death and follicular atresia in ewe ovaries.T heriogenology. 2024 Sep 15;226:236-242

    

   1. Gioia L, Palazzese L, Czernik M, Iuso D, Fulka H, Fulka J Jr, Loi P. Oocyte activation is a cytoplasm-confined event so far: what about the nucleus? 2024 Feb 2;167(3):e230360

    

   1. Palazzese L, Turri F, Anzalone DA, Saragusty J, Bonnet J, Colotte M, Tuffet S, Pizzi F, Luciani A, Matsukawa K, Czernik M, Loi P Reviving vacuum-dried encapsulated ram spermatozoa via ICSI after 2 years of storage. Front Vet Sci. 2023 Nov 30;10:1270266.

    

    

   1. Palazzese L, Czernik M, Matsukawa K,Loi PSomatic Cell Nuclear Transfer Using Freeze-Dried Protaminized Donor Nuclei.  Methods Mol Biol. 2023;2647:211-224

    

   1. Fulka H,Loi P, Czernik M, Surani A, Fulka JOmne vivum ex ovo: the oocyte reprogramming and remodeling activities. 2023 Jan 12;165(3):R75-R89

    

    

   1. Thiangthientham P, Kallayanathum W, Anakkul N, Suwimonteerabutr J, Santiviparat S, Techakumphu M,Loi P, Tharasanit Effects of freeze-drying on the quality and fertilising ability of goat sperm recovered from different parts of the epididymis. 2023 Jan 1;195:31-39.

    

   1. Czernik M, Winiarczyk D, Sampino S, Gręda P, Parillo S, Modliński JA,Loi PMitochondrial function and intracellular distribution is severely affected in in vitro cultured mouse embryos. Sci Rep. 2022 Sep 27;12(1):16152. doi: 10.1038/s41598-022-20374-6

    

   1. Capra E, Toschi P, Del Corvo M, Lazzari B, Stella A, Williams JL,Loi P, Ajmone Marsan P.Maternal undernutrition during periconceptional period affects whole-genome ovine muscle methylation in adult offspring. J Anim Sci. 2022 Aug 1;100(8):skac180

    

    

   1. Fulka H,Loi P, Palazzese L, Benc M, Fulka Jr J JrNucleus reprogramming/remodeling through selective enucleation (SE) of immature oocytes and zygotes: a nucleolus point of view.J Reprod Dev. 2022 Jun 1;68(3):165-172.

    

   1. Comizzoli P, Loi P, Patrizio P, Hubel A. Long-term storage of gametes and gonadal tissues at room temperatures: the end of the ice age? J Assist Reprod Genet. 2022 Feb;39(2):321-325

    

   1. Fulka H, Loi P, Palazzese L, Benc M, Fulka Jr J Jr Nucleus reprogramming/remodeling through selective enucleation (SE) of immature oocytes and zygotes: a nucleolus point of view. J Reprod Dev. 2022 Jun 1;68(3):165-172

    

   1. Anzalone DA, Palazzese L, Czernik M, Sabatucci A, Valbonetti L, Capra E, Loi P Controlled spermatozoa-oocyte interaction improves embryo quality in sheep. Sci Rep. 2021 Nov 19;11(1):22629

    

   1. Hildebrandt TB, Hermes R, Goeritz F, Appeltant R, Colleoni S, de Mori B, Diecke S, Drukker M, Galli C, Hayashi K, Lazzari G, Loi P, Payne J, Renfree M, Seet S, Stejskal J, Swegen A, Williams SA, Zainuddin ZZ, Holtze S. The ART of bringing extinction to a freeze - History and future of species conservation, exemplified by rhinos. 2021 Jul 15;169:76-88.

    

   1. Capra E, Toschi P, Del Corvo M, Lazzari B, Stella A, Williams JL, Loi P, Ajmone Marsan P Short Communication: Maternal undernutrition during peri-conceptional period affects whole genome ovine muscle methylation in adult offspring. J Anim Sci. 2022 May 17:skac180. doi: 10.1093/jas/skac180

    

   1. Dahlen CR, Borowicz PP, Ward AK, Caton JS, Czernik M, Palazzese L, Loi P, Reynolds LP Programming of Embryonic Development. Int J Mol Sci. 2021 Oct 28;22(21):11668

    

   1. Rychtarova J, Langerova A, Fulka H, Loi P, Benc M, Fulka J Jr Interspecific ICSI for the Assessment of Sperm DNA Damage: Technology Report. Animals (Basel). 2021 Apr 26;11(5):1250

    

   1. Loi P, Palazzese L, Scapolo PA, Fulka J, Fulka H, Czernik M 25th ANNIVERSARY OF CLONING BY SOMATIC-CELL NUCLEAR TRANSFER: Scientific and technological approaches to improve SCNT efficiency in farm animals and pets. 2021 Jun 11;162(1):F33-F43

    

   1. Ressaissi Y, Anzalone DA, Palazzese L, Czernik M, Loi P The impaired development of sheep ICSI derived embryos is not related to centriole dysfunction. 2021 Jan 1;159:7-12

    

   1. Palazzese L, Anzalone DA, Turri F, Faieta M, Donnadio A, Pizzi F, Pittia P, Matsukawa K, Loi P Whole genome integrity and enhanced developmental potential in ram freeze-dried spermatozoa at mild sub-zero temperature. Sci Rep. 2020 Nov 2;10(1):18873

    

   1. Helena Fulka, Jana Rychtarova & Loi P. The nucleolus-like and precursor bodies of mammalian oocytes and 1 embryos and their possible role in post-fertilisation centromere remodelling.  Biochemical Society Transactions, 2020, pii: BST20190847. doi: 10.1042/BST20190847

    

   1. Czernik M, Fidanza A, Luongo FP, Valbonetti L, Scapolo PA, Patrizio P,Loi P. Late Embryogenesis Abundant (LEA) proteins confer water stress tolerance to mammalian somatic cells. Cryobiology. 2020 Jan 14. pii: S0011-2240(19)30232-9

    

   1. Saragusty J, Anzalone DA, Palazzese L, Arav A, Patrizio P, Gosálvez J,Loi P.  Dry biobanking as a conservation tool in the Anthropocene. Theriogenology. 2020 Jan 13. pii: S0093-691X(20)30028-5.

    

   1. Toschi P, Capra E, Anzalone DA, Lazzari B, Turri F, Pizzi F, Scapolo PA, Stella A, Williams JL, Ajmone Marsan P,Loi P. Maternal peri-conceptional undernourishment perturbs offspring sperm methylome. Reproduction. 2020 May;159(5):513-523

    

   1. Marta Czernik, Debora A. Anzalone, Luca Palazzese, Mami Oikawa, Pasqualino Loi (2019). Somatic cell nuclear transfer: failures, successes and the challenges ahead. THE INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, ISSN: 0214-6282, doi: 10.1387/ijdb.180324mc

    

   1. Fulka H, Ogura A,Loi P, Fulka Jr J Jr.  Dissecting the role of the germinal vesicle nuclear envelope and soluble content in the process of somatic cell remodelling and reprogramming. J Reprod Dev. 2019 Oct 23;65(5):433-441

    

   1. Saragusty J,Loi P.  Exploring dry storage as an alternative biobanking strategy inspired by Nature. Theriogenology. 2019 Mar 1;126:17-27

    

   1. Fulka JJ, Benc M,Loi P, Langerova A, Fulka H. Function of atypical mammalian oocyte/zygote nucleoli and its implications for reproductive biology and medicine. Int J Dev Biol. 2019;63(3-4-5):105-112

    

   1. Luca Palazzese, Jaime Gosálvez, Debora A. Anzalone, Pasqualino Loi, Joseph Saragusty (2018). DNA fragmentation in epididymal freeze-dried ram spermatozoa impairs embryo development. JOURNAL OF REPRODUCTION AND DEVELOPMENT, ISSN: 0916-8818, doi: 10.1262/jrd.2018-033

    

   1. Debora Agata Anzalone, Luca Palazzese, Domenico Iuso, Giuseppe Martino, Pasqualino Loi (2018). Freeze-dried spermatozoa: An alternative biobanking option for endangered species. ANIMAL REPRODUCTION SCIENCE, vol. 190, p. 85-93, ISSN: 0378-4320, doi: 10.1016/j.anireprosci.2018.01.010
   2. Loi P, Galli C, Lazzari G, Matsukawa K, Fulka J Jr, Goeritz F, Hildebrandt TB. Development to term of sheep embryos reconstructed after inner cell mass/trophoblast exchange. J Reprod Dev. 2018 Apr 13;64(2):187-191

    

   1. Hildebrandt TB, Hermes R, Colleoni S, Diecke S, Holtze S, Renfree MB, Stejskal J, Hayashi K, Drukker M,Loi P, Göritz F, Lazzari G, Galli C. Embryos and embryonic stem cells from the white rhinoceros. Nat Commun. 2018 Jul 4;9(1):2589.

    

   1. Luca Palazzese, Marta Czernik, Domenico Iuso, Paola Toschi, Pasqualino Loi (2018). Nuclear quiescence and histone hyper-acetylation jointly improve protamine-mediated nuclear remodeling in sheep fibroblasts. PLOS ONE, vol. 13, ISSN: 1932-6203, doi: 10.1371/journal.pone.0193954

    

   1. LOI, Pasqualino, IUSO, DOMENICO, TOSCHI, PAOLA, PALAZZESE, LUCA, CZERNIK, Marta Teresa (2017). Alternative strategies for nuclear reprogramming in somatic cell nuclear transfer (SCNT). ANIMAL REPRODUCTION, vol. 14, p. 377-382, ISSN: 1806-9614, doi: 10.21451/1984-3143-AR915

    

   1. Capra E., Toschi P., Del Corvo M. D., Lazzari B., Scapolo P. A., Loi P., Williams J. L., Stella A., Ajmone-Marsan P. (2017). Genome-wide epigenetic characterization of tissues from three germ layers isolated from sheep fetuses. FRONTIERS IN GENETICS, vol. 8, 115, ISSN: 1664-8021, doi: 10.3389/fgene.2017.00115

    

   1. Loi Pasqualino, Iuso Domenico, Czernik Marta, Ogura Atsuo (2016). A New, Dynamic Era for Somatic Cell Nuclear Transfer?. TRENDS IN BIOTECHNOLOGY, vol. 34, p. 791-797, ISSN: 0167-7799, doi: 10.1016/j.tibtech.2016.03.008

    

   1. Toschi Paola, Czernik Marta, Zacchini Federica, Fidanza Antonella, Loi Pasqualino, Ptak Grazyna Ewa (2016). Evidence of placental autophagy during early pregnancy after transfer of in vitro produced (IVP) sheep embryos. PLOS ONE, vol. 11, e0157594, ISSN: 1932-6203, doi: 10.1371/journal.pone.0157594

    

    

   1. ANZALONE, DEBORA AGATA, IUSO, DOMENICO, CZERNIK, Marta Teresa, PTAK, Grazyna, LOI, Pasqualino (2016). Plasma membrane and acrosome loss before ICSI is required for sheep embryonic development. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, vol. 33, p. 757-763, ISSN: 1573-7330, doi: 10.1007/s10815-016-0709-1

    

   1. Czernik Marta, Iuso Domenico, Toschi Paola, Khochbin Saadi, Loi Pasqualino (2016). Remodeling somatic nuclei via exogenous expression of protamine 1 to create spermatid-like structures for somatic nuclear transfer. NATURE PROTOCOLS, vol. 11, p. 2170-2188, ISSN: 1754-2189, doi: 10.1038/nprot.2016.130

    

    

   1. Saragusty Joseph, Diecke Sebastian, Drukker Micha, Durrant Barbara, Friedrich Ben-Nun Inbar, Galli Cesare, Göritz Frank, Hayashi Katsuhiko, Hermes Robert, Holtze Susanne, Johnson Stacey, Lazzari Giovanna, Loi Pasqualino, Loring Jeanne F., Okita Keisuke, Renfree Marilyn B., Seet Steven, Voracek Thomas, Stejskal Jan, Ryder Oliver A., Hildebrandt Thomas B (2016). Rewinding the process of mammalian extinction. ZOO BIOLOGY, vol. 35, p. 280-292, ISSN: 0733-3188, doi: 10.1002/zoo.21284

    

   1. Loi Pasqualino, Toschi Paola, Zacchini Federica, Ptak Grazyna, Scapolo Pier A., Capra Emanuele, Stella Alessandra, Marsan Paolo Ajmone, Williams John L. (2016). Synergies between assisted reproduction technologies and functional genomics. GENETICS SELECTION EVOLUTION, vol. 48, 48, ISSN: 0999-193X, doi: 10.1186/s12711-016-0231-z

    

   1. Iuso Domenico, Czernik Marta, Toschi Paola, Fidanza Antonella, Zacchini Federica, Feil Robert, Curtet Sandrine, Buchou Thierry, Shiota Hitoshi, Khochbin Saadi, Ptak Grazyna Ewa, Loi Pasqualino (2015). Exogenous Expression of Human Protamine 1 (hPrm1) Remodels Fibroblast Nuclei into Spermatid-like Structures. CELL REPORTS, vol. 13, p. 1765-71-1771, ISSN: 2211-1247, doi: 10.1016/j.celrep.2015.10.066

    

   1. Cree L, Loi P (2015). Mitochondrial replacement: from basic research to assisted reproductive technology portfolio tool-technicalities and possible risks. MOLECULAR HUMAN REPRODUCTION, vol. 21, ISSN: 1360-9947, doi: 10.1093/molehr/gau082

    

   1. Ptak Grazyna E, Toschi Paola, Fidanza Antonella, Czernik Marta, Zacchini Federica, Modlinski Jacek A, Loi Pasqualino (2014). Autophagy and apoptosis: parent-of-origin genome-dependent mechanisms of cellular self-destruction. OPEN BIOLOGY, vol. 4, ISSN: 2046-2441, doi: 10.1098/rsob.140027

    

   1. S Sampino, G R Juszczak, F Zacchini, A H Swiergiel, J A Modlinski, P Loi, G E Ptak (2014). Grand-paternal age and the development of autism-like symptoms in mice progeny. TRANSLATIONAL PSYCHIATRY, vol. 4, ISSN: 2158-3188, doi: 10.1038/tp.2014.27

    

   1. Fidanza A, Toschi P, Zacchini F, Czernik M, Palmieri C, Scapolo Pa, Modlinski Ja, Loi P, Ptak G (2014). Impaired placental vasculogenesis compromises the growth of sheep embryos developed in vitro. BIOLOGY OF REPRODUCTION, vol. 91, ISSN: 0006-3363, doi: 10.1095/biolreprod.113.113902

    

   1. Iuso D, Czernik M, Zacchini F, Ptak G, Loi P (2013). A simplified approach for oocyte enucleation in mammalian cloning.. CELLULAR REPROGRAMMING, ISSN: 2152-4971, doi: 10.1089/cell.2013.0051

    

   1. Ptak GE, Modlinski JA, Loi P (2013). Embryonic diapause in humans: time to consider?. REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, ISSN: 1477-7827, doi: 10.1186/1477-7827-11-92

    

   1. Iuso D, Czernik M, Di Egidio F, Sampino S, Zacchini F, Bochenek M, Smorag Z, Modlinski JA, Ptak G, Loi P (2013). Genomic stability of lyophilized sheep somatic cells before and after nuclear transfer.. PLOS ONE, ISSN: 1932-6203, doi: 10.1371/journal.pone.0051317

    

   1. Loi P, Czernik M, Zacchini F, Iuso D, Scapolo PA, Ptak G (2013). Sheep: the first large animal model in nuclear transfer research.. CELLULAR REPROGRAMMING, ISSN: 2152-4971, doi: 10.1089/cell.2013.0032

    

   1. Fulka J Jr, Langerova A, Loi P, Ptak G, Albertini D, Fulka H (2013). The ups and downs of somatic cell nucleus transfer (SCNT) in humans.. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, ISSN: 1058-0468, doi: 10.1007/s10815-013-0053-7

    

   1. Loi, D. Iuso, M. Czernik, F. Zacchini, G. Ptak (2013). Towards storage of cells and gametes in dry form. TRENDS IN BIOTECHNOLOGY, vol. 31, p. 688-695, ISSN: 0167-7799, doi: 10.1016/j.tibtech.2013.09.004

    

   1. Czernik M, Fidanza A, Sardi M, Galli C, Brunetti D, Malatesta D, Della Salda L, Matsukawa K, Ptak G, Loi P (2013). Differentiation potential and GFP labeling of sheep bone marrow derived mesenchymal stem cells.. JOURNAL OF CELLULAR BIOCHEMISTRY, ISSN: 0730-2312, doi: 10.1002/jcb.24310.

    

   1. Iuso D, Czernik M, Di Egidio F, Sampino S, Zacchini F, Bochenek M, Smorag Z, Modlinski JA, Ptak G, Loi P (2013). Genomic stability of lyophilized sheep somatic cells before and after nuclear transfer. PLOS ONE, vol. Epub 2013 Jan 8., p. 1-10, ISSN: 1932-6203, doi: 10.1371/journal.pone.0051317

    

   1. Ptak GE, D'Agostino A, Toschi P, Fidanza A, Zacchini F, Czernik M, Monaco F, Loi P (2013). Post-implantation mortality of in vitro produced embryos is associated with DNA methyltransferase 1 dysfunction in sheep placenta. HUMAN REPRODUCTION, vol. 28, p. 298-305, ISSN: 0268-1161, doi: doi: 10.1093/humrep/des397

    

   1. Ptak G, Zacchini F, Czernik M, Fidanza A, Palmieri C, Della Salda L, Scapolo PA, Loi P (2012). A short exposure to polychlorinated biphenyls deregulates cellular autophagy in mammalian blastocyst in vitro. . HUMAN REPRODUCTION, vol. 27, p. 1034-1042, ISSN: 0268-1161, doi: doi: 10.1093/humrep/der454

    

   1. Ptak GE, Tacconi E, Czernik M, Toschi P, Modlinski JA, LOI P (2012). Embryonic diapause is conserved across mammals.. PLOS ONE, vol. 7, p. 1-7, ISSN: 1932-6203, doi: http://dx.crossref.org/10.1371%2Fjournal.pone.0033027

    

   1. Loi P, Teruhiko Wakayama, Josef Fulka Jr and Grazyna Ptak. (2011). Biological time machines: a realistic approach for cloning an extinct mammal.. ENDANGERED SPECIES RESEARCH, vol. Vol. 14: , p. 227-233, ISSN: 1613-4796

    

   1. Zacchini F, Czernik M, Iuso D, Toschi P, di Egidio F, Scapolo PA, Loi P, Ptak G. (2011). Efficient production and cellular characterization of sheep androgenetic embryos. . CELLULAR REPROGRAMMING, vol. Dec;13(6), p. 495-502, ISSN: 2152-4971

    

   1. Loi P, Fulka J Jr, Hildebrand T, Ptak G. (2011). Genome of non-living cells: trash or recycle? . REPRODUCTION, vol. Oct;142(4), p. 497-503, ISSN: 1470-1626

    

    

   1. Loi P, Modlinski JA, Ptak G. (2011). Interspecies somatic cell nuclear transfer: a salvage tool seeking first aid. . THERIOGENOLOGY, vol. Jul 15;76(2), p. 217-228, ISSN: 0093-691

    

   1. Fulka J Jr, Langerova A, Loi P, Martinkova S, Fulka H. (2011). Transplantation of nucleoli into human zygotes: not as simple as expected? . JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, vol. 28(5), p. 385, ISSN: 1058-0468

    

   1. FULKA JR J, LOI P, PTAK G, FULKA H, JOHN JS (2009). Hope for the Mammoth?. CLONING AND STEM CELLS, ISSN: 1536-2302

    

   1. LOI P, BEAUJEAN N, KHOCHBIN S, FULKA J JR, PTAK G (2008). Asymmetric nuclear reprogramming in somatic cell nuclear transfer?. BIOESSAYS, vol. 30, p. 66-74, ISSN: 0265-9247

    

   1. FULKA J JR, FULKA H, ST JOHN J, GALLI C, LAZZARI G, LAGUTINA I, FULKA J, LOI P (2008). Cybrid human embryos--warranting opportunities to augment embryonic stem cell research. TRENDS IN BIOTECHNOLOGY, vol. 26, p. 469-474, ISSN: 0167-779

    

   1. LOI P, MATSUKAWA K, PTAK G, CLINTON M, FULKA J JR, NATHAN Y, ARAV A (2008). Freeze-dried somatic cells direct embryonic development after nuclear transfer. PLOS ONE, vol. e2978., ISSN: 1932-6203

    

    

   1. TURCO MY, MATSUKAWA K, CZERNIK M, GASPERI V, BATTISTA N, DELLA SALDA L, SCAPOLO PA, LOI P, MACCARRONE M, PTAK G (2008). High levels of anandamide, an endogenous cannabinoid, block the growth of sheep preimplantation embryos by inducing apoptosis and reversible arrest of cell proliferation. HUMAN REPRODUCTION, vol. 23, p.

    

   1. LOI P, KAZUTSUGU MATSUKAWA SEIYA TAKAHASHI, SATOSHI AKAGI GRAZYNA PTAK (2008). Nuclear reprogramming: what has been done and potential avenues for improvements. CENTRAL EUROPEAN JOURNAL OF BIOLOGY, vol. 3, p. 211-223, ISSN: 1895-104X

    

    

   1. LOI P, MATZUKAWA K, PTAK G, NATAN Y, FULKA J JR, ARAV A (2008). Nuclear transfer of freeze-dried somatic cells into enucleated sheep oocytes. REPRODUCTION IN DOMESTIC ANIMALS, vol. 43, p. 417-422, ISSN: 0936-6768

    

    

   1. PALMIERI C, LOI P, PTAK G, DELLA SALDA L (2008). Review paper: a review of the pathology of abnormal placentae of somatic cell nuclear transfer clone pregnancies in cattle, sheep, and mice. VETERINARY PATHOLOGY, vol. 45, p. 865-880, ISSN: 0300-9858

    

    

   1. LOI P, GALLI C, PTAK G (2007). Cloning of endangered mammalian species: any progress?. TRENDS IN BIOTECHNOLOGY, vol. 25, p. 195-200, ISSN: 0167-7799

    

   1. Matsukawa K, Turco MY, Scapolo PA, Reynolds L, Ptak G, Loi P (2007). Development of sheep androgenetic embryos is boosted following transfer of male pronuclei into androgenetic hemizygotes. . CLONING AND STEM CELLS, vol. 9, p. 374-381, ISSN: 1536-2302

    

    

   1. PALMIERI C, LOI P, REYNOLDS LP, PTAK G, DELLA SALDA L (2007). Placental abnormalities in ovine somatic cell clones at term: a light and electron microscopic investigation. PLACENTA, vol. 28, p. 577-584, ISSN: 0143-4004

    

    

   1. PTAK G, MATSUKAWA K, PALMIERI C, DELLA SALDA L, SCAPOLO PA, LOI P (2006). Developmental and functional evidence of nuclear immaturity in prepubertal oocytes. HUMAN REPRODUCTION, vol. 21, p. 2228-2237, ISSN: 0268-1161

    

    

   1. GRAZYNA PTAK, FEDERICA LOPES, KAZUTSUGU MATSUKAWA, MARIAN TISCHNER, PASQUALINO LOI (2006). Leukemia Inhibitory Factor enhances sheep ferilization in vitro via an influence on the oocyte. THERIOGENOLOGY, p. 1-10, ISSN: 0093-691X

    

    

   1. LOI P, CLINTON, M., VACKOVA, I., FULKA JR., J., FEIL, R., PALMIERI, DELLA SALDA, L., AND PTAK G (2006). Placental abnormalities associated with post-natal mortality in sheep somatic cell clones. THERIOGENOLOGY, vol. 65 , p. 1110-1121, ISSN: 0093-691X, doi: doi:10.1016/j.theriogenology.2005.07.016

    

   1. IRENA VACKOVA, RADOMIR KREN, PASQUALINO LOI, VLADIMR KRYLOV AND JOSEF FULKA, LOI P (2006). The absence of DNA replication checkpoint in porcine zygotes. ZYGOTE, vol. 14, p. 33-37, ISSN: 0967-1994

    

   1. FULKA J JR, MRAZEK M, FULKA H, LOI P (2005). Mammalian oocyte therapies. CLONING AND STEM CELLS, vol. 7, p. 183-188, ISSN: 1536-2302

    

    

   1. LOI P (2004). Are there realistic opportunities to apply cloning and the other reproductive biotechnologies for safeguarding endangered species?. ANIMAL SCIENCE PAPERS AND REPORTS, vol. 22, p. 81-89, ISSN: 0860-4037

    

   1. FULKA J JR, LOI P, FULKA H, PTAK G, NAGAI T. (2004). Nucleus transfer in mammals: noninvasive approaches for the preparation of cytoplasts. TRENDS IN BIOTECHNOLOGY, vol. 22, p. 27-283, ISSN: 0167-7799

    

   1. MATTIOLI M, GIOIA L, TURRIANI M, CAPACCHIETTI G, LOI P, PTAK G. (2004). Oocyte maturation is required for correct sperm chromatin rearrangement. VETERINARY RESEARCH COMMUNICATIONS, vol. 28, p. 205-208, ISSN: 0165-7380

    

   1. LOI P, DELLA SALDA L., PTAK G. (2004). Peri and post natal mortality of somatic cell clones in sheep. ANIMAL SCIENCE PAPERS AND REPORTS, vol. 22, suppl. 1, p. 59-70, ISSN: 0860-4037

    

   1. LOI P, DELLA SALDA, L., PTAK G. (2004). Peri- and post-natal mortality of somatic cell clones in sheep. ANIMAL SCIENCE PAPERS AND REPORTS, vol. 22, p. 59-63, ISSN: 0860-4037

    

   1. BEAUJEAN N, TAYLOR JE, MCGARRY M, GARDNER JO, WILMUT I, LOI P, PTAK G, GALLI C, LAZZARI G, BIRD A, YOUNG LE, MEEHAN RR (2004). The effect of interspecific oocytes on demethylation of sperm DNA. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 20, p. 7636-7640, ISSN: 1091-6490

    

   1. LOI P, FULKA J JR, PTAK G (2003). Amphibian and mammal somatic-cell cloning: different species, common results? (Cover page). TRENDS IN BIOTECHNOLOGY, vol. 21, p. 471-473, ISSN: 0167-7799

    

   1. MATTIOLI M, BARBONI B, GIOIA L, LOI P, LUISA G (2003). Cold-induced calcium elevation triggers DNA fragmentation in immature pig oocytes. MOLECULAR REPRODUCTION AND DEVELOPMENT, vol. 65, p. 289-297, ISSN: 1040-452X

    

   1. YOUNG LE, SCHNIEKE AE, MCCREATH KJ, WIECKOWSKI S, KONFORTOVA G, FERNANDES K, PTAK G, KIND AJ, WILMUT I, LOI P, FEIL R. (2003). Conservation of IGF2-H19 and IGF2R imprinting in sheep: effects of somatic cell nuclear transfer. MECHANISMS OF DEVELOPMENT, vol. 120, p. 1433-1442, ISSN: 0925-4773

    

   1. PTAK GRAZYNA, TISCHENR MARIAN, BERNABÒ NICOLA, LOI P (2003). Donor-Dependent Developmental Competence of Oocytes from Lambs Subjected to Repeated Hormonal Stimulation. BIOLOGY OF REPRODUCTION, vol. 2003 Feb 5, ISSN: 0006-3363

    

    

   1. FULKA J, MOOR RM, LOI P, FULKA J (2003). Enucleolation of porcine oocytes. THERIOGENOLOGY, vol. 59, p. 1879-1885, ISSN: 0093-691X

    

    

   1. FULKA J JR, LOI P, FULKA H, KREN R, DEAN W, MRAZEK M, REIK W (2003). Nucleus replacement in Mammalian oocytes. CLONING AND STEM CELLS, vol. 4, p. 181-187, ISSN: 1536-2302

    

   1. LOI P, BARBONI B, PTAK G (2002). Cloning advances and challenges for conservation. TRENDS IN BIOTECHNOLOGY, vol. 20, p. 233, ISSN: 0167-7799

    

   1. PTAK GRAZYNA, CLINTON MICHAEL, TISHNER MARIAN, MATTIOLI MAURO, BARBONI BARBARA, LOI P (2002). Improving delivery and offspring viability of in vitro-produced and cloned sheep embryos. BIOLOGY OF REPRODUCTION, vol. 67, p. 1719-1725, ISSN: 0006-3363

    

   1. LOI P, CLINTON M., BARBONI B., FULKA J. JR., CAPPAI P., FEIL R., MOOR R.H.M., PTAK G. (2002). Nuclei of nonviable ovine somatic cells develop into lambs after nuclear transplantation. BIOLOGY OF REPRODUCTION, vol. 67, p. 442-450, ISSN: 0006-3363

    

   1. PTAK G., CLINTON M., BARBONI B., MUZZEDDU M., CAPPAI P., TISCHNER M., LOI P (2002). Preservation of the wild European mouflon: the first example of genetic management using a complete program of reproductive biotechnologies. BIOLOGY OF REPRODUCTION, vol. 66, p. 796-801, ISSN: 0006-3363

    

    

   1. LOI P, PTAK GRAZYNA, BARBONI BARBARA, FULKA JOSEF JR, CAPPAI PIETRO, MICHAEL CLINTON (2001). Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells. NATURE BIOTECHNOLOGY, vol. 19, p. 962-964, ISSN: 1087-0156

    

    

   1. FULKA J. JR, LOI P, LEDDA S, MOOR RHM, FULKA J (2001). Nucleus transfer in mammals: how the oocyte cytoplasm modifies the transferred nucleus. THERIOGENOLOGY, vol. 55, p. 1373-1380, ISSN: 0093-691X

    

   1. FULKA JR, J. TESARIK, LOI P, R.M. MOOR (2000). Manipulating the Human embryo: Cell Cycle Checkpoint Controls. CLONING AND STEM CELLS, vol. 2, p. 1-7, ISSN: 1536-2302

    

    

   1. LOI P, FULKA J.JR., NAITANA S., CAPPAI P. (1999). Cloning by nuclear transfer: experiences in sheep. LIVESTOCK PRODUCTION SCIENCE, vol. 60, p. 281-294, ISSN: 0301-6226

    

   1. PTAK G, LOI P, DATTENA M, TISCHNER M, CAPPAI P (1999). Offspring from one-month-old lambs: studies on the developmental capability of prepubertal oocytes. BIOLOGY OF REPRODUCTION, vol. 61, p. 1568-1574, ISSN: 0006-336

    

    

   1. PTAK G., DATTENA M., LOI P, TISCHNER M., CAPPAI P (1999). Ovum pick-up in sheep: efficiency of in vitro embryo production, vitrification and offspring. THERIOGENOLOGY, vol. 52, p. 1105-1114, ISSN: 0093-691X

    

   1. LOI P, PTAK G., DATTENA M., LEDDA S., NAITANA S., CAPPAI P (1998). Embryo transfer and related technologies in sheep reproduction. REPRODUCTION NUTRITION DEVELOPMENT, vol. 38, p. 615-628, ISSN: 0926-5287

    

   1. FULKA J JR, FIRST NL, LOI P, MOOR RHM (1998). Cloning by somatic cell nuclear transfer. BIOESSAYS, vol. 20, p. 847-851, ISSN: 0265-9247

    

   1. LOI P, LEDDA S., FULKA J.JR., CAPPAI P., MOOR R.M. (1998). Development of parthenogenetic and cloned ovine embryos: effect of activation protocols. BIOLOGY OF REPRODUCTION, vol. 58, p. 1177-1187, ISSN: 0006-3363

    

   1. FEIL R, KOSHLA S, CAPPAI P, LOI P (1998). Genomic imprinting in ruminants: allele-specific gene expression in parthenogenetic sheep. MAMMALIAN GENOME, vol. 9, p. 831-834, ISSN: 0938-8990

    

   1. NAITANA S., LEDDA S., LEONI G., BOGLIOLO L., LOI P, CAPPAI P (1998). Membrane integrity and fertilizing potential of cryopreserved spermatozoa in European mouflon. ANIMAL REPRODUCTION SCIENCE, vol. 52, p. 105-112, ISSN: 0378-4320

    

    

   1. LEDDA S, BOGLIOLO L, LEONI G, LOI P, CAPPAI P, NAITANA S (1998). Two culture systems showing a biphasic effect on ovine embryo development from the 1-2 cell stage to hatched blastocysts. REPRODUCTION NUTRITION DEVELOPMENT, vol. 35, p. 629-637, ISSN: 0926-5287

    

   1. CAMPBELL K.H.S., LOI P, OTAEGUI P., WILMUT I (1997). Cell cycle co-ordination in embryo cloning by nuclear transfer. JOURNAL OF REPRODUCTION AND FERTILITY, vol. 1, p. 40-46, ISSN: 0022-4251

    

   1. LOI P, BOYAZOGLU S., GALLUS M., BRANCA A., LEDDA S., NAITANA S., WILMUT I., CAPPAI P (1997). Embryo Cloning in Sheep: work in progress. THERIOGENOLOGY, vol. 48, p. 1-10, ISSN: 0093-691X

    

   1. NAITANA S, LEDDA S, LOI P, LEONI G, BOGLIOLO L, DATTENA M, CAPPAI P (1997). Polyvinyl alcohol as a defined substitute for serum in vitrification and warming solutions to cryopreserve ovine embryos at different stages of development. ANIMAL REPRODUCTION SCIENCE, vol. 48, p. 247-256, ISSN: 0378-4320

    

   1. NAITANA S, LEDDA S, LOI P, LEONI G, BOGLIOLO L, DATTENA M, CAPPAI P (1997). Polyvinyl alcohol as a definite substitute for serum in vitrification and warming solutions to cryopreserve ovine embryos at different stages of development. ANIMAL REPRODUCTION SCIENCE, vol. 48, p. 247-256, ISSN: 0378-4320

    

   1. CAMPBELL KH, LOI P, OTAEGUI PJ, WILMUT I (1996). Cell cycle co-ordination in embryo cloning by nuclear transfer. REVIEWS OF REPRODUCTION, vol. 1, p. 40-46, ISSN: 1359-6004

    

   1. NAITANA S, LOI P, LEDDA S, CAPPAI P, DATTENA M, BOGLIOLO L, LEONI G (1996). Effect of biopsy and vitrification on in vitro survival of ovine embryos at different stages of development. THERIOGENOLOGY, vol. 46, p. 813-824, ISSN: 0093-691X

    

   1. LEDDA S., LOI P, BOGLIOLO L., MOOR R.M. FULKA J. JR. (1996). The effect of 6- Dimethylaminopurine (6-DMAP) on DNA synthesis in activated mammalian oocytes. ZYGOTE, vol. 4, p. 7-9, ISSN: 0967-1994

    

    

   1. CAMPBELL K.H.S., LOI P, CAPPAI P., WILMUT I (1994). Improved development to blastocyst of ovine nuclear transfer embryos reconstructed during the presumptive S-Phase of enucleated-activated oocytes. BIOLOGY OF REPRODUCTION, vol. 50, p. 1385-1393, ISSN: 0006-3363

    

    

    

    

Funding ID

CORDINATOR, Horizon Europe, WhyNotDry, MSC SE,2023-2027, 780000 euro

COORDINATOR, H2020, MSCA RISE 2016-2021 GA 734434. Setting an interdisciplinary/sectorial/international research network to explore dry storage as an alternative strategy for cells/germplasm biobanking.  1530000 €

COORDINATOR,H2020 MSCA IF 2017-2019. GA  749537, Drystore. Exploring dry storage as an alternative biobanking strategy inspired by Nature. 188000 €

PI H2020 Twinning Action 2016-2019. GA 698165. Epigenetic Risk Assessment of Assisted Reproductive Technologies. 295000 €

COORDINATOR, MIUR PRIN 2009: I 2009JE3CHM Induction and evaluation of multipotency in embryonic… of veterinary interest, 370,000 €

COORDINATOR,MIUR PRIN 2006 Project: 2006078015_002 Cell therapy: development of large animal models for bio-medical and pre-clinical studies, 147000 €

COORDINATOR,PMIUR PRIN 2002: cod. 2002074357 Isolation and characterization of adult and embryonic stem cells… differentiation plasticity 115,000 €

COORDINATOR,MIUR FIRB Project Negotiating Projects 2001: RBNE01HPMX Identification of mechanisms that regulate the expression of genes of … of domestic animals . 1250000 €

PI Project FP7-KBBE-2009-3: Next generation methods to preserve farm animal biodiversity by optimizing present and future breeding options. 135,000 €

PI European Science Foundation grant "Stellar" 2004-007 "Novel approaches to enhance…. research" acronym; EuroSTELLS, VI FP EU contract no. ERAS-CT-2003-980409; 154000 €

COORDINATOR, MIUR Interlink: II04C0H723 Study of the expression of single genes (DNMT1), and of the…. post implantation period. 45000 €

PI United States Department of Agriculture (USDA) "Proposal # 2007-01215

"Deregulation of Placenta vascular development in ART-derived sheep fetuses, $ 350,000

PI MIUR FIRB Establishing a Genome Resort for animal breeding and selection. GenHome cod. 0070120.  678,000 €

COORDINATOR,Tercas Foundation "Development of protocols for the freeze-drying of cell lines and embryos ", 100,000 €